-
公开(公告)号：US11613761B1
公开(公告)日：2023-03-28
申请号：US15526550
申请日：2015-11-13
Inventor： Toralf Senger , Laurent Marty , Irene Kunze , Heiko Haertel , Steven Craig Bremmer , Steven D. Breazeale , Joerg Bauer , Patricia Vrinten , Sten Stymne , Jenny Lindberg Yilmaz , John A. McElver , Dietrich Rein , Carl Andre
Abstract: T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.
-
公开(公告)号：US11484560B2
公开(公告)日：2022-11-01
申请号：US15525463
申请日：2015-11-13
Applicant: BASF PLANT SCIENCE COMPANY GMBH
Inventor： Toralf Senger , Heiko A. Haertel
IPC: C12N15/82 , A61K36/31 , C12Q1/6895 , C12N9/02 , C12N9/00 , A61K31/202
Abstract: Assay method, comprising providing a plant capable of expressing a delta-12 desaturase, wherein said delta-12 desaturase has at least 50% total amino acid sequence identity to at least one of the sequences SEQ ID NO. 328 to 336, and/or at least 59% total amino acid sequence similarity to at least one of the sequences SEQ ID NO. 328 to 336, and wherein the plant is also capable of expressing at least one or more enzymes of unsaturated fatty acid metabolism, of which enzymes at least one is capable of using linoleic acid as a substrate, and of which enzymes at least one is supposedly connected to a plant metabolic property, growing the plant, and measuring said plant metabolic property for said plant.
-
公开(公告)号：US20210308202A1
公开(公告)日：2021-10-07
申请号：US17346522
申请日：2021-06-14
Applicant: BASF PLANT SCIENCE COMPANY GMBH
Inventor： Toralf Senger , Laurent Marty , Irene Kunze , Dietrich Rein , Jörg Bauer , Carl Andre
IPC: A61K36/31 , C12Q1/6895
Abstract: The present invention provides transgenic Brassica events LBFLFK and LBFDAU and progeny thereof, and cells, seeds, plants comprising DNA diagnostic for these events. The invention also provides artificial oligonucleotide primers and probes that are diagnostic for the LBFLFK and LBFDAU events and their progeny in a sample, and methods for detecting the presence of the LBFLFK and LBFDAU events and their progeny in a sample. The invention further provides oil and commodity products derived from the LBFLFK and LBFDAU events.
-
公开(公告)号：US10731169B2
公开(公告)日：2020-08-04
申请号：US14902715
申请日：2014-07-03
Applicant: BASF PLANT SCIENCE COMPANY GMBH
Inventor： Toralf Senger , Joerg Bauer
IPC: C12N15/82 , C12N15/113
Abstract: The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field.For the production of desired molecules in plant cells, e.g. PUFAs, it is frequently required to express a target gene heterologous to the plant cell, or to overexpress a target gene naturally found in said plant cell.
-
公开(公告)号：US10208270B2
公开(公告)日：2019-02-19
申请号：US15159931
申请日：2016-05-20
Applicant: BASF Plant Science Company GmbH
Inventor： Toralf Senger , Laurent Marty , Sten Stymne , Jenny Lindberg Yilmaz , Johnathan A. Napier , Olga Sayanova , Richard Haslam , Ruiz Lopez Noemi
IPC: C12N15/82 , C11C1/04 , A23D9/02 , C12P7/64 , A23K20/158 , A23L33/105 , A23L33/115 , A23L33/12
Abstract: The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.
-
Patent Agency Ranking
16.发明申请DESATURASES AND PROCESS FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 有权在转基因生物中生产聚氨酯脂肪酸的苦味和过程公开(公告)号：US20140335579A1
公开(公告)日：2014-11-13
申请号：US14338673
申请日：2014-07-23
Applicant: BASF Plant Science Company GmbH
Inventor： Toralf Senger , Jörg Bauer , Johnathan A. Napier
CPC classification number: C12P7/6409 , C12N9/0071 , C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472 , C12P7/6481 , C12Y114/19001
Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerin and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.
Abstract translation: 本发明涉及来自嗜球菌（Cocchioiobolus heterostrophus）C5，蓝藻（Cyanothece sp。）的多核苷酸 CCY0110，真菌孢子菌和寄生藻类，代表去饱和酶，可用于重组生产多不饱和脂肪酸。 本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。 本发明还涉及针对根据本发明的多肽的抗体。 最后，本发明还涉及多不饱和脂肪酸和油，脂和脂肪酸组合物的生产方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品添加剂的用途。
-
公开(公告)号：US20190270972A1
公开(公告)日：2019-09-05
申请号：US16414150
申请日：2019-05-16
Inventor： Toralf Senger , Patricia Vrinten , Ze Long Lim
Abstract: The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.
-
公开(公告)号：US10329541B2
公开(公告)日：2019-06-25
申请号：US15105080
申请日：2014-12-17
Applicant: BASF PLANT SCIENCE COMPANY GMBH
Inventor： Toralf Senger , Patricia Vrinten , Ze Long Lim
Abstract: The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.
-
公开(公告)号：US20180291389A1
公开(公告)日：2018-10-11
申请号：US15525463
申请日：2015-11-13
Applicant: BASF PLANT SCIENCE COMPANY GMBH
Inventor： Toralf Senger , Heiko A. Haertel
Abstract: Assay method, comprising providing a plant capable of expressing a delta-12 desaturase, wherein said delta-12 desaturase has at least 50% total amino acid sequence identity to at least one of the sequences SEQ ID NO. 328 to 336, and/or at least 59% total amino acid sequence similarity to at least one of the sequences SEQ ID NO. 328 to 336, and wherein the plant is also capable of expressing at least one or more enzymes of unsaturated fatty acid metabolism, of which enzymes at least one is capable of using linoleic acid as a substrate, and of which enzymes at least one is supposedly connected to a plant metabolic property, growing the plant, and measuring said plant metabolic property for said plant.
-
公开(公告)号：US09879234B2
公开(公告)日：2018-01-30
申请号：US14416981
申请日：2013-07-30
Applicant: BASF PLANT SCIENCE COMPANY GMBH
Inventor： Laurent Marty , Toralf Senger
CPC classification number: C12N9/0071 , C12N9/001 , C12P7/6427 , C12Y114/19
Abstract: The invention provides nucleic acid molecules which encodes a novel fatty acid desaturase, KCS, KCR and/or LACS from Thraustochytrium aureum and Sphaeroforma arctica. The invention also provides recombinant expression vectors containing the nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA and for screening for delta-4 desaturases.
-
-
-
-
-
-
-
-
-
Search Results
28
records
(took 0.03 seconds)
