公开(公告)号：US11198882B2

公开(公告)日：2021-12-14

申请号：US16112200

申请日：2018-08-24

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US11149287B2

公开(公告)日：2021-10-19

申请号：US16246864

申请日：2019-01-14

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US20200224269A1

公开(公告)日：2020-07-16

申请号：US16819972

申请日：2020-03-16

Applicant: Dow AgroSciences LLC

Inventor： PoHao Wang ,  Shreedharan Sriram ,  Lakshmi Sastry-Dent

IPC: C12Q1/6876 ,  G16B25/00 ,  C12Q1/6895 ,  C12N15/82 ,  G16B20/00

Abstract: The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold value for predicting gene silencing. The methods and/or systems provided herein can be used in high-throughput setting for screening large number of transformed event in a relatively short period of time as compared to existing technologies.

公开(公告)号：US10233465B2

公开(公告)日：2019-03-19

申请号：US14531732

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10106804B2

公开(公告)日：2018-10-23

申请号：US15867770

申请日：2018-01-11

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  W. Michael Ainley

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US20130211729A1

公开(公告)日：2013-08-15

申请号：US13761711

申请日：2013-02-07

Applicant: Dow Agrosciences LLC

Inventor： Lakshmi Sastry-Dent ,  Shreedharan Sriram ,  Navin Elango ,  Zehui Cao ,  Karthik Narayan Muthuraman

IPC: G06F19/28

CPC classification number: G16B50/00 ,  G16B30/00

Abstract: Systems and methods for data analysis are provided. In one embodiment, a method for analysis is provided, including electronically receiving sequence data; electronically receiving one or more reference data sequences related to at least an expression vector; associating the sequence data with at least one of the reference data sequences to identify a transgene flanking sequence; searching a genome for one or more insertion sites of the transgene flanking sequence; and annotating the genome and the one or more insertion sites within the genome when one or more insertion sites are found in said searching step.

Abstract translation: 提供了数据分析的系统和方法。 在一个实施例中，提供了一种用于分析的方法，包括电子地接收序列数据; 电子地接收与至少一个表达载体相关的一个或多个参考数据序列; 将序列数据与至少一个参考数据序列相关联以鉴定转基因侧翼序列; 在基因组中搜索转基因侧翼序列的一个或多个插入位点; 并且当在所述搜索步骤中发现一个或多个插入位点时，注释基因组和基因组内的一个或多个插入位点。