公开(公告)号：US20200087671A1

公开(公告)日：2020-03-19

申请号：US16698077

申请日：2019-11-27

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/79 ,  C12N15/82 ,  C12N15/63 ,  C12N9/22

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US10344322B2

公开(公告)日：2019-07-09

申请号：US15249684

申请日：2016-08-29

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Matthew A. Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6895 ,  C12N15/10 ,  C12N15/66 ,  C12N15/70 ,  C40B40/08 ,  A01G24/00 ,  C12N5/00

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

公开(公告)号：US20180371478A1

公开(公告)日：2018-12-27

申请号：US16123326

申请日：2018-09-06

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  W. Michael Ainley

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10160975B2

公开(公告)日：2018-12-25

申请号：US14458086

申请日：2014-08-12

Applicant: Dow AgroSciences LLC

Inventor： W. Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC: C12N15/82

Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

公开(公告)号：US20170044559A1

公开(公告)日：2017-02-16

申请号：US15292774

申请日：2016-10-13

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： W. Michael Ainley ,  Steven R. Webb ,  Jayakumar P. Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC: C12N15/82 ,  C07K14/47 ,  C12N9/22

CPC classification number: C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

Abstract: Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

Abstract translation: FAD2基因座中的基因破坏，基因编辑或基因堆叠的方法和组合，通过以位点方式切割大豆细胞中FAD2基因的位置，在FAD2基因中产生断裂，然后任选地整合入 揭示目的核酸分子。

公开(公告)号：US20200071712A1

公开(公告)日：2020-03-05

申请号：US16674831

申请日：2019-11-05

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： W. Michael Ainley ,  Dmitry Y. Guschin ,  Matthew Hayden ,  Daniel Isenegger ,  John Mason ,  Jeffrey C. Miller ,  Joseph F. Petolino ,  Yidong Ran ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb

IPC: C12N15/82 ,  C12N15/90

Abstract: Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

公开(公告)号：US10577616B2

公开(公告)日：2020-03-03

申请号：US15943106

申请日：2018-04-02

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N15/90

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US10273493B2

公开(公告)日：2019-04-30

申请号：US14531705

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  Navin Elango

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US20190085344A1

公开(公告)日：2019-03-21

申请号：US16209028

申请日：2018-12-04

Applicant: Dow AgroSciences LLC

Inventor： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC: C12N15/82

Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

公开(公告)号：US09493844B2

公开(公告)日：2016-11-15

申请号：US14105223

申请日：2013-12-13

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Matthew Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  A01G24/00 ,  C12N5/00 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/70 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C40B40/08

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。