公开(公告)号：US20160108443A1

公开(公告)日：2016-04-21

申请号：US14975670

申请日：2015-12-18

Applicant: DSM IP Assets B.V.

Inventor： Paul W. BEHRENS ,  John M. THOMPSON ,  Kirk APT ,  Joseph W. PFEIFER ,  James P. WYNN ,  James Casey LIPPMEIER ,  Jaouad FICHTALI ,  Jon HANSEN

IPC: C12P7/64 ,  C11B1/10

CPC classification number: C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract translation: 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

公开(公告)号：US20150132803A1

公开(公告)日：2015-05-14

申请号：US14548054

申请日：2014-11-19

Applicant: DSM IP ASSETS B.V.

Inventor： Kirk E. APT ,  James Casey LIPPMEIER ,  David SIMPSON ,  Jun WANG ,  James P. WYNN ,  Ross ZIRKLE

IPC: C07K14/705 ,  C12N15/79

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract translation: 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。