公开(公告)号：US20220380759A1

公开(公告)日：2022-12-01

申请号：US17817899

申请日：2022-08-05

Applicant: DSM IP ASSETS B.V.

Inventor： James Casey LIPPMEIER ,  Yelena BETZ ,  Johannes Andries ROUBOS ,  Rene VERWAAL

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/86 ,  C12P21/00

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.

公开(公告)号：US20140142331A1

公开(公告)日：2014-05-22

申请号：US14165459

申请日：2014-01-27

Applicant: DSM IP ASSETS B.V.

Inventor： Paul W. BEHRENS ,  John M. THOMPSON ,  Kirk APT ,  Joseph W. PFEIFER ,  James P. WYNN ,  James Casey LIPPMEIER ,  Jaouad FICHTALI ,  Jon HANSEN

IPC: C12P7/64

CPC classification number: C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract translation: 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

公开(公告)号：US20190177754A1

公开(公告)日：2019-06-13

申请号：US16273273

申请日：2019-02-12

Applicant: DSM IP Assets B.V.

Inventor： James Casey LIPPMEIER ,  Joseph W. PFEIFER, III ,  Jon Milton HANSEN ,  Kirk E. APT ,  William Robert BARCLAY ,  Paul Warren BEHRENS ,  David Christian MARTIN

IPC: C12P7/64 ,  C10L1/02

Abstract: The present invention provides biological oils and methods and uses thereof. The biological oils are preferably produced by heterotrophic fermentation of one or more microorganisms using cellulose-containing feedstock as a main source of carbon. The present invention also provides methods of producing lipid-based biofuels and food, nutritional, and pharmaceutical products using the biological oils.

公开(公告)号：US20180171310A1

公开(公告)日：2018-06-21

申请号：US15579751

申请日：2016-06-06

Applicant: DSM IP Assets B.V.

Inventor： James G. METZ ,  Jun WANG ,  Bastien CHEVREUX ,  Ross E. ZIRKLE ,  Anne-Cecile BAYNE ,  James Casey LIPPMEIER

IPC: C12N9/10 ,  C12P7/64 ,  C12N15/82 ,  A23L33/115 ,  A23K20/158

Abstract: This disclosure concerns a novel modular docosahexaenoic acid (DHA) synthase and recombinant host organisms genetically modified with such synthase and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US20170145461A1

公开(公告)日：2017-05-25

申请号：US15342623

申请日：2016-11-03

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. APT ,  James Casey LIPPMEIER ,  David SIMPSON ,  Jun WANG ,  James P. WYNN ,  Ross ZIRKLE

IPC: C12P21/00 ,  C12R1/90 ,  C12N9/26 ,  C12N15/80

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

公开(公告)号：US20140106402A1

公开(公告)日：2014-04-17

申请号：US14108794

申请日：2013-12-17

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. APT ,  James Casey LIPPMEIER ,  David SIMPSON ,  Jun WANG ,  James P. WYNN ,  Ross ZIRKLE

IPC: C12P21/00 ,  C12R1/90

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract translation: 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

公开(公告)号：US20170183669A1

公开(公告)日：2017-06-29

申请号：US15400318

申请日：2017-01-06

Applicant: DSM IP ASSETS B.V.

Inventor： James Casey LIPPMEIER ,  Kirk E. Apt ,  Jon Milton Hansen

IPC: C12N15/79 ,  A23L2/52 ,  A23K20/10 ,  A23L33/10 ,  C12N1/10 ,  A23K50/80

CPC classification number: C12N15/79 ,  A23K20/10 ,  A23K50/80 ,  A23L2/52 ,  A23L33/10 ,  A23V2002/00 ,  C12N1/10 ,  C12N1/12 ,  C12N9/2405 ,  C12P7/6463 ,  C12P7/649 ,  C12Y302/01026 ,  C12Y302/01048 ,  Y02E50/13

Abstract: The present invention is directed to recombinant thraustochytrids that grow on sucrose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.

公开(公告)号：US20160355851A1

公开(公告)日：2016-12-08

申请号：US15237988

申请日：2016-08-16

Applicant: DSM IP Assets B.V.

Inventor： James Casey LIPPMEIER ,  Joseph W. PFEIFER, III ,  Jon Milton HANSEN, III ,  Kirk E. APT ,  William Robert BARCLAY ,  Paul Warren BEHRENS ,  David Christian MARTIN

IPC: C12P7/64 ,  C10L1/02

CPC classification number: C12P7/6472 ,  C10L1/02 ,  C10L1/026 ,  C10L2200/0469 ,  C10L2270/026 ,  C10L2270/04 ,  C10L2290/26 ,  C12P7/649 ,  Y02E50/13 ,  Y02P30/20

Abstract: The present invention provides biological oils and methods and uses thereof. The biological oils are preferably produced by heterotrophic fermentation of one or more microorganisms using cellulose-containing feedstock as a main source of carbon. The present invention also provides methods of producing lipid-based biofuels and food, nutritional, and pharmaceutical products using the biological oils.

Abstract translation: 本发明提供生物油及其方法和用途。 生物油优选通过使用含纤维素原料作为主要碳源的一种或多种微生物的异养发酵产生。 本发明还提供了使用生物油制备基于脂质的生物燃料和食品，营养和药物产品的方法。

公开(公告)号：US20190211369A1

公开(公告)日：2019-07-11

申请号：US16249253

申请日：2019-01-16

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. APT ,  James Casey LIPPMEIER ,  David SIMPSON ,  Jun WANG ,  James P. WYNN ,  Ross ZIRKLE

IPC: C12P21/00 ,  C12N15/79 ,  C12R1/90 ,  C12N15/80 ,  C07K14/705 ,  C12N9/26 ,  C07K14/37

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

公开(公告)号：US20170191023A1

公开(公告)日：2017-07-06

申请号：US15400224

申请日：2017-01-06

Applicant: DSM IP ASSETS B.V.

Inventor： James Casey LIPPMEIER ,  Kirk E. Apt

IPC: C12N1/10 ,  C12N15/52 ,  A23K50/00 ,  A23L33/10 ,  A23K50/80 ,  C12N1/22 ,  A23L2/52

CPC classification number: C12N1/10 ,  A23K50/00 ,  A23K50/80 ,  A23L2/52 ,  A23L33/10 ,  A23V2002/00 ,  C12N1/22 ,  C12N15/52 ,  C12N15/79 ,  C12P7/649 ,  Y02E50/13

Abstract: The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.