公开(公告)号：US20210198701A1

公开(公告)日：2021-07-01

申请号：US17199823

申请日：2021-03-12

Applicant: DSM IP ASSETS B.V.

Inventor： Paul KLAASSEN ,  Gijsberdina Pieternella VAN SUYLEKOM ,  Bianca Elisabeth Maria GIELESEN ,  Nicolette Jasmijn BROERS ,  Beate WIEDEMANN ,  Wilhelmus Theodorus Antonius Maria DE LAAT

IPC: C12P7/10 ,  C12P7/46

Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.

公开(公告)号：US20210071205A1

公开(公告)日：2021-03-11

申请号：US17096751

申请日：2020-11-12

Applicant: DSM IP ASSETS B.V.

Inventor： Hans Marinus Charles Johannes DE BRUIJN ,  Wilhelmus Theodorus Antonius Maria DE LAAT ,  Paul KLAASSEN

IPC: C12P7/10 ,  C12P7/06 ,  C07C31/08

Abstract: The invention relates to a process for producing a fermentation product that comprises fermentation of a carbon source in a reactor with a cell, capable of converting sugar, glycerol and acetic acid, wherein the carbon source comprises sugar and acetic acid, comprising the following steps: a) Inoculating a optionally diluted carbon source with the cell; b) optionally fermenting the reactor in batch mode; c) adding carbon source comprising glycerol and optionally sugar gradually to the reactor; d) after sufficient fermentation time, isolation of fermentation product from the reactor, e) optionally keeping the remaining fraction after isolation of step d) as spent broth; and f) optionally using the spent broth in step a) to dilute the carbon source.

公开(公告)号：US20190330664A1

公开(公告)日：2019-10-31

申请号：US16509700

申请日：2019-07-12

Applicant: DSM IP ASSETS B.V.

Inventor： Paul KLAASSEN ,  Wouter Willem Antonius HARTMAN

IPC: C12P7/10 ,  C12N1/16 ,  C12N9/02 ,  C12N9/12 ,  C12P7/06 ,  C12N9/04 ,  C12N9/00

Abstract: Cell that is genetically modified comprising: a) one or more nucleotide sequence encoding a NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); b) one or more nucleotide sequence encoding a acetyl-CoA synthetase (E.C. 6.2.1.1); c) one or more nucleotide sequence encoding a glycerol dehydrogenase (E.C. 1.1.1.6); and d) one or more nucleotide sequence encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 or E.C. 2.7.1.29).

公开(公告)号：US20160208291A1

公开(公告)日：2016-07-21

申请号：US14914612

申请日：2014-08-29

Applicant: DSM IP ASSETS B.V.

Inventor： Paul KLAASSEN ,  Antonius Willem Wouter HARTMAN

IPC: C12P7/10 ,  C12N9/12 ,  C12N9/00 ,  C12N9/04 ,  C12P7/06 ,  C12N9/02

Abstract: Cell that is genetically modified comprising: a) one or more nucleotide sequence encoding a NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); b) one or more nucleotide sequence encoding a acetyl-CoA synthetase (E.C. 6.2.1.1); c) one or more nucleotide sequence encoding a glycerol dehydrogenase (E.C. 1.1.1.6); and d) one or more nucleotide sequence encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 or E.C. 2.7.1.29).

Abstract translation: 经遗传修饰的细胞包含：a）编码NAD +依赖性乙酰化乙醛脱氢酶（E.C.1.2.1.10）的一个或多个核苷酸序列; b）一个或多个编码乙酰辅酶A合成酶的核苷酸序列（E.C.6.2.1.1）; c）编码甘油脱氢酶（E.C.1.1.1.6）的一个或多个核苷酸序列; 和d）编码二羟基丙酮激酶（E.C.2.7.1.28或E.C.2.7.1.29）的一个或多个核苷酸序列。

公开(公告)号：US20160122400A1

公开(公告)日：2016-05-05

申请号：US14895742

申请日：2014-06-04

Applicant: DSM IP ASSETS B.V.

Inventor： Paul KLAASSEN ,  Paulus Petrus DE WAAL ,  Rene Marcel DE JONG ,  Arnold Jacob Mattieu DRIESSEN ,  Jeroen Gerben NIJLAND ,  Hyun Yong SHIN

IPC: C07K14/395 ,  C12P19/02

CPC classification number: C07K14/395 ,  C12P19/02

Abstract: The invention relates to a polypeptide having one or more substitution at a position corresponding to position 339 or 376 of SEQ ID NO: 59, wherein the polypeptide is a member of the Major Facilitator Superfamily (MFS). In an embodiment, the substitution is at position corresponding to 376 and wherein the amino acid at that position is replaced by an amino acid that has a van der Waals volume of 80 to 138 Å3 and a side chain hydrophobicity of 10 to 100 ΔtR.

Abstract translation: 本发明涉及在对应于SEQ ID NO：59的位置339或376的位置具有一个或多个取代的多肽，其中多肽是主要促进剂超家族（MFS）的成员。 在一个实施方案中，取代位于对应于376的位置，并且其中该位置处的氨基酸被具有范德华体积为80至138埃的氨基酸和10至100重量％的侧链疏水性 。