公开(公告)号：US12049617B2

公开(公告)日：2024-07-30

申请号：US17050626

申请日：2019-05-21

Applicant: DSM IP Assets B.V.

Inventor： Christopher Mark Farrell ,  Lisa Ann Laprade ,  Otto Martin Lehmann ,  Joshua Trueheart ,  Bastien Jean Wolfgang Chevreux

IPC: C12N1/16 ,  C12N9/90 ,  C12P33/00

CPC classification number: C12N1/16 ,  C12N9/90 ,  C12P33/00

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

公开(公告)号：US10385308B2

公开(公告)日：2019-08-20

申请号：US14772137

申请日：2014-03-13

Applicant: DSM IP Assets B.V.

Inventor： Joshua Trueheart ,  Jessica McGrath

IPC: C12P7/22 ,  C12N9/22 ,  C12Q1/68 ,  C12N1/08 ,  C12P23/00

Abstract: The present invention relates to the use of a thermophilic nuclease for degrading nucleic acids in vivo and/or in situ, wherein the thermophilic nuclease is heterologous to the host cell and is produced by the host rather than being added exogenously. The present invention further relates to a genetically modified cell which was produced according to the above method. The present invention is particularly beneficial in inactivating the biological activity of recombinant DNA in biomass or biomass-derived products.

公开(公告)号：US09297031B2

公开(公告)日：2016-03-29

申请号：US14183268

申请日：2014-02-18

Applicant: DSM IP ASSETS B.V.

Inventor： Richard B. Bailey ,  Kevin T. Madden ,  Joshua Trueheart ,  Reed Doten ,  Maria Mayorga ,  Joshua Griffin Dunn ,  Dan Dueppen

IPC: C12N1/19 ,  C12P23/00 ,  C12N15/52

CPC classification number: C12P23/00 ,  C12N15/52

Abstract: The present invention provides systems for producing engineered oleaginous yeast or fungi that express retinolic compounds.

Abstract translation: 本发明提供用于生产工程化含油酵母或表达视黄醛化合物的真菌的系统。