公开(公告)号：US07470532B2

公开(公告)日：2008-12-30

申请号：US11253882

申请日：2005-10-19

Applicant: Daniel Joseph Macool ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Daniel Joseph Macool ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: C12N1/20 ,  C12N9/10 ,  C12N1/21 ,  C07H21/04 ,  C12P7/64

CPC classification number: C12N9/1029 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: The present invention relates to a fungal C16/18 fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of a Mortierella alpina C16/18 fatty acid elongase is provided (designated as “ELO3”). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C16/18 fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such as Yarrowia lipolytica.

Abstract translation: 本发明涉及能够催化棕榈酸酯（16:0）转化成硬脂酸（18:0）的真菌C16 / 18脂肪酸延伸酶。 具体地，提供了被孢霉烯C16 / 18脂肪酸延伸酶的核苷酸序列（称为“ELO3”）。 本文描述了增加微生物油生产，增加碳通量进入多不饱和脂肪酸生物合成途径并通过过表达C16 / 18脂肪酸延伸酶增加多不饱和脂肪酸的含量的方法。 最希望的是，即时ELO3的底物特异性对于能够在含油酵母，如解脂耶氏酵母（Yarrowia lipolytica）中积累长链多不饱和脂肪酸将是特别有用的。