公开(公告)号：US20180273995A1

公开(公告)日：2018-09-27

申请号：US15537763

申请日：2015-12-18

申请人： Danisco US Inc.

发明人： Vivek Sharma ,  Paula Johanna Maria Teunissen

IPC分类号： C12P19/02 ,  C12P19/14 ,  C12P7/08 ,  C12C11/00

CPC分类号： C12P19/02 ,  C12C11/003 ,  C12G3/02 ,  C12P7/08 ,  C12P7/10 ,  C12P19/14 ,  C12Y302/01003 ,  Y02E50/16

摘要： Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of Fusarium verticillioides glucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.

公开(公告)号：US20160333332A1

公开(公告)日：2016-11-17

申请号：US15112664

申请日：2015-01-15

申请人： DANISCO US INC.

发明人： Paula Johanna Maria Teunissen ,  Michael J. Pepsin ,  Vivek Sharma

IPC分类号： C12N9/24 ,  C12P19/14 ,  C12P19/02 ,  C12P7/64

CPC分类号： C12N9/2482 ,  C11B1/025 ,  C11B13/00 ,  C12P7/06 ,  C12P7/64 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01008 ,  Y02E50/17 ,  Y02W30/74

摘要： Provided are GH10 xylanases or a fragment thereof including modified xlyanase enzymes, all having xylanase activity, wherein the enzyme or fragment thereof provides increased oil recovery from a grain-based material compared with a control or parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at at least one or more of the following positions 7, 25, 33, 64, 79, 89, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). Methods of using the GH10 xylanases, for example, for improved oil recovery are also provided.

摘要翻译： 提供的GH10木聚糖酶或其片段包括全部具有木聚糖酶活性的改性的xlyanase酶，其中与对照或亲本GH10木聚糖酶相比，其酶或片段提供了与谷物基材料相比提高的油回收率，母本GH10木聚糖酶已被 在以下至少一个或多个位置7,25,33,64,79,89,217和298处修饰，其中编号基于FveXyn4（SEQ ID No.1）的氨基酸编号。 还提供了使用GH10木聚糖酶的方法，例如用于改进的油回收。

公开(公告)号：US20130115659A1

公开(公告)日：2013-05-09

申请号：US13737820

申请日：2013-01-09

申请人： DANISCO US INC.

发明人： Suzanne Breneman ,  Thomas S. Green ,  Michael Jay Pepsin ,  Vivek Sharma ,  Jayarama K. Shetty

IPC分类号： C12P19/02 ,  C12P19/14

CPC分类号： C12N9/16 ,  C12P19/14 ,  Y02E50/17

摘要： Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment.

摘要翻译： 描述了与没有植酸酶预处理步骤并且不调整浆料pH调节的淀粉加工相关的组合物和方法。