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公开(公告)号：US11920170B2
公开(公告)日：2024-03-05
申请号：US16060506
申请日：2016-12-09
申请人： Danisco US Inc.
发明人： William Cuevas , Vivek Sharma , David E. Wildes , Sang-Kyu Lee , Dina Finan
CPC分类号： C12N9/2417 , C11D3/386 , C12N9/2414 , C12P19/14 , C12Y302/01001
摘要： Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
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公开(公告)号：US11130975B2
公开(公告)日：2021-09-28
申请号：US16745760
申请日：2020-01-17
申请人： DANISCO US INC
摘要： Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of Fusarium verticillioides glucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.
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3.发明授权Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids 有权直接淀粉可发酵糖作为生产异戊二烯，类异戊二烯前体分子和/或类异戊二烯的原料公开(公告)号：US09315831B2
公开(公告)日：2016-04-19
申请号：US13802420
申请日：2013-03-13
发明人： Collette M. Blake , Gopal K. Chotani , Gang Duan , Douglas Ko , Floor Kooy , Sung Ho Lee , Michael J. Pepsin , Ying Qian , Matt Reboli , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Pauline Johanna Maria Teunissen , Hongxian Xu
CPC分类号： C12P5/007 , C12N9/88 , C12P7/42 , C12P7/46 , C12P7/56 , C12P19/02 , C12P19/14 , C12Y301/03 , Y02P20/52
摘要： Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism, such as isoprene, isoprenoid precursor molecules, and/or isoprenoids. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.
摘要翻译： 本文提供的组合物和方法与将研磨的或分级的颗粒中的淀粉直接转化成能够通过发酵生物体如异戊二烯工业生产一种或多种产物的碳源的可发酵糖原料有关， 类异戊二烯前体分子和/或类异戊二烯。 这样的转化可以在等于或低于晶粒中存在的淀粉的初始糊化温度的温度下进行，并且可以利用存在于某些未精制谷物中的一种或多种可分离的内源性酶。
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公开(公告)号：US20150079660A1
公开(公告)日：2015-03-19
申请号：US14494436
申请日：2014-09-23
申请人： DANISCO US INC.
发明人： Vivek Sharma , Jayarama Shetty , Bruce Strohm
CPC分类号： C07H1/08 , C12N9/2417 , C12P19/14
摘要： The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variant thereof. AmyE or its variants thereof may be used to eliminate or reduce the iodine-positive starch presented in saccharide liquor. Also disclosed are a composition comprising an AmyE or variant thereof and a method utilizing an AmyE or variant thereof to eliminate or reduce the iodine-positive starch
摘要翻译： 本公开涉及枯草芽孢杆菌α-淀粉酶（AmyE）或其变体。 可以使用AmyE或其变体来消除或减少糖液中存在的碘阳性淀粉。 还公开了包含AmyE或其变体的组合物和利用其AmyE或其变体来消除或减少碘阳性淀粉的方法
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5.发明申请ALPHA-AMYLASE BLEND FOR STARCH PROCESSING AND METHOD OF USE THEREOF 有权用于淀粉加工的ALPHA-AMYLASE共混物及其使用方法公开(公告)号：US20140087429A1
公开(公告)日：2014-03-27
申请号：US14014046
申请日：2013-08-29
申请人： Danisco US Inc.
摘要： The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.
摘要翻译： 本公开涉及包含低pH，热稳定性α-淀粉酶和地衣芽孢杆菌α-淀粉酶的酶共混物。 混合物可以包含低pH，热稳定性α-淀粉酶的每5.0个改性Wohlgemuth单位（MWU）的地衣芽孢杆菌α-淀粉酶的至少约1.0Liquefon Unit（LU）。 描述的酶混合物适用于淀粉液化和糖化，乙醇生产和/或甜味剂生产等。 本文还提供了通过用低pH，热稳定性α-淀粉酶和地衣芽孢杆菌α-淀粉酶同时或依次液化淀粉来处理淀粉的方法。
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公开(公告)号：US20180163191A1
公开(公告)日：2018-06-14
申请号：US15423457
申请日：2017-02-02
申请人： Danisco US Inc.
发明人： William Cuevas , Vivek Sharma , David E. Wildes , Sang-kyu Lee , Dina Finan
CPC分类号： C12N9/2417 , C11D3/386 , C12N9/2414 , C12P19/14 , C12Y302/01001
摘要： Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
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公开(公告)号：US20150152457A1
公开(公告)日：2015-06-04
申请号：US14479683
申请日：2013-03-13
申请人： Danisco US Inc.
发明人： Collette Michelle Blake , Gang Duan , Douglas Ko , Floor K. Kooy , Sung Ho Lee , Michael Jay Pepsin , Ying Qian , Matthew T. Reboli , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
CPC分类号： C12P19/14 , C12P19/02 , C12P19/12 , C12P19/20 , C12Y301/03 , C12Y302/01001 , C12Y302/01003
摘要： Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.
摘要翻译： 本文提供的是与将研磨或分馏的谷粒中的淀粉直接转化成可发酵糖原料相关的组合物和方法，其可以作为发酵生物体工业生产一种或多种产品的碳源。 这样的转化可以在等于或低于晶粒中存在的淀粉的初始糊化温度的温度下进行，并且可以利用存在于某些未精制谷物中的一种或多种可分离的内源性酶。
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公开(公告)号：US08883457B2
公开(公告)日：2014-11-11
申请号：US13737820
申请日：2013-01-09
申请人： Danisco US Inc
摘要： Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment.
摘要翻译： 描述了与没有植酸酶预处理步骤并且不调整浆料pH调节的淀粉加工相关的组合物和方法。
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9.发明授权公开(公告)号：US08815560B2
公开(公告)日：2014-08-26
申请号：US14014046
申请日：2013-08-29
申请人： Danisco US Inc.
摘要： The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.
摘要翻译： 本公开涉及包含低pH，热稳定性α-淀粉酶和地衣芽孢杆菌α-淀粉酶的酶共混物。 混合物可以包含低pH，热稳定性α-淀粉酶的每5.0个改性Wohlgemuth单位（MWU）的地衣芽孢杆菌α-淀粉酶的至少约1.0Liquefon Unit（LU）。 描述的酶混合物适用于淀粉液化和糖化，乙醇生产和/或甜味剂生产等。 本文还提供了通过用低pH，热稳定性α-淀粉酶和地衣芽孢杆菌α-淀粉酶同时或依次液化淀粉来处理淀粉的方法。
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公开(公告)号：US20190144842A1
公开(公告)日：2019-05-16
申请号：US16060506
申请日：2016-12-09
申请人： Danisco US Inc.
发明人： William Cuevas , Vivek Sharma , David E. Wildes , Sang-Kyu Lee , Dina Finan
摘要： Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
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