公开(公告)号：US20150366757A1

公开(公告)日：2015-12-24

申请号：US14311289

申请日：2014-06-21

Applicant: David Clements

Inventor： David Clements

IPC: A61J1/18 ,  A61B19/00

CPC classification number: A61J1/18 ,  A61B90/90 ,  A61B90/92 ,  A61B90/94 ,  A61B90/96 ,  A61J1/00 ,  A61J1/035 ,  A61J1/10 ,  A61J2205/20 ,  A61J2205/50 ,  A61M5/31525 ,  A61M2005/3126

Abstract: An apparatus comprises a container comprising a volume. The volume is configured to retain a single dose of a medication appropriate for a specific classification of a weight of a patient. A first indicia is disposed on an exterior surface of the container. The first indicia indicates a type of the medication. A second indicia is disposed on the exterior surface. The second indicia indicates the specific classification, wherein the container, pre-packaged with the single dose, is appropriate for a single use dosing of the patient having the specific classification.

Abstract translation: 一种装置包括容器，其包括容积。 体积被配置为保留适合于患者重量的特定分类的单剂量的药物。 第一标记设置在容器的外表面上。 第一个标记表示一种药物。 第二标记设置在外表面上。 第二标记表示具体分类，其中用单次剂量预先包装的容器适合于具有特定分类的患者的单次使用剂量。

公开(公告)号：US20070042002A1

公开(公告)日：2007-02-22

申请号：US11506991

申请日：2006-08-16

Applicant: Carolyn Weeks-Levy ,  David Clements ,  Steven Ogata

Inventor： Carolyn Weeks-Levy ,  David Clements ,  Steven Ogata

IPC: A61K39/145 ,  C07H21/04 ,  C12N7/00 ,  C12N5/06 ,  C12N15/86 ,  C07K14/11

CPC classification number: A61K39/145 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/55505 ,  A61K2039/55577 ,  A61K2039/70 ,  C07K14/005 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2800/105

Abstract: The invention provides influenza proteins, including subunit proteins and immunogenic compositions that can be utilized, with or without adjuvants, as vaccines to protect against influenza infection in animal models and humans. The recombinant proteins are expressed from transformed insect cells that contain integrated copies of the appropriate expression cassettes in their genome. The invention uses a Drosophila melanogaster expression system to provide high yields of recombinant subunit proteins with native-like conformation.

公开(公告)号：US6136561A

公开(公告)日：2000-10-24

申请号：US937195

申请日：1997-09-25

Applicant: John Ivy ,  Eilen Nakano ,  David Clements

Inventor： John Ivy ,  Eilen Nakano ,  David Clements

IPC: A61K39/00 ,  C07K14/18 ,  C07K16/10 ,  C12N15/09

CPC classification number: C07K14/005 ,  C07K16/1081 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/02 ,  C12N2770/24122 ,  G01N2333/18

Abstract: The Flaviviridae comprise a number of medically important pathogens that cause significant morbidity in humans including the dengue (DEN) virus, Japanese encephalitis (JE) virus, tick-borne encephalitis virus (TBE), and yellow fever virus (YF). Flaviviruses are generally transmitted to vertebrates by chronically infected mosquito or tick vectors. The viral particle which is enveloped by host cell membranes, comprises a single positive strand genomic RNA and the structural capsid (CA), membrane (M), and envelope (E) proteins. The E and M proteins are found on the surface of the virion where they are anchored in the membrane. Mature E is glycosylated and contains functional domains responsible for cell surface attachment and intraendosomal fusion activities. Problems have arisen in the art with respect to producing recombinant forms of the E glycoprotein that retain their native configuration and attendant properties associated therewith (i.e., ability to induce neutralizing antibody responses). To date, recombinantly produced E glycoproteins have suffered from a number of limitations including improper glycosylation, folding, and disulfide bond formation. The claimed invention has addressed these concerns by providing secreted recombinant forms of the E glycoprotein that are highly immunogenic and appear to retain their native configuration. Carboxy-terminally truncated forms of E containing the amino terminal 395 amino acids and a suitable secretion signal sequence were generated in Drosophila melanogaster Schneider cell lines. The recombinant proteins produced by this expression system should prove useful, inter alia, as immunogens and diagnostic reagents.

Abstract translation: 黄病毒科包括许多在医学上重要的病原体，其引起人类重大的发病率，包括登革热（DEN）病毒，日本脑炎（JE）病毒，蜱传脑炎病毒（TBE）和黄热病病毒（YF）。 黄病毒一般通过慢性感染的蚊子或蜱载体传播给脊椎动物。 由宿主细胞膜包围的病毒颗粒包含单个正链基因组RNA和结构衣壳（CA），膜（M）和包膜（E）蛋白。 E和M蛋白存在于病毒粒子的表面，它们被锚定在膜中。 成熟的E被糖基化并且包含负责细胞表面附着和体内融合活性的功能结构域。 关于生产重组形式的E糖蛋白，其保留其天然构型和与其相关的伴随性质（即，诱导中和抗体应答的能力）方面出现了问题。 迄今为止，重组产生的E糖蛋白已经受到许多限制，包括不正确的糖基化，折叠和二硫键形成。 所要求保护的发明通过提供高度免疫原性并且看起来保持其天然构型的E糖蛋白的分泌重组形式来解决这些问题。 在黑腹果蝇施耐德细胞系中产生含有氨基末端395个氨基酸和合适的分泌信号序列的E的羧基末端截短形式。 由该表达系统产生的重组蛋白尤其应用于免疫原和诊断试剂。