公开(公告)号：US20050059014A1

公开(公告)日：2005-03-17

申请号：US10653386

申请日：2003-09-03

申请人： Michael Pawlak ,  Eginhard Schick ,  Peter Oroszlan

发明人： Michael Pawlak ,  Eginhard Schick ,  Peter Oroszlan

IPC分类号： G01N33/566 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N21/76 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553

CPC分类号： G01N33/54353 ,  G01N33/54373

摘要： The present invention is related to an analytical platform and a method performed therewith for the analysis of a multitude of “nature-identical” samples for analytes which are contained therein and are of biological relevance as binding partners in specific binding reactions, wherein said samples or dilutions of said samples of the same relative molecular composition as the original samples, with the analytes to be determined contained therein, as a first plurality of specific binding partners, are deposited in discrete measurement areas in at least one one- or two-dimensional array of measurement areas on an evanescent field sensor platform as a solid support without a change in the relative molecular composition, compared with the original relative molecular composition of the sample, one or more tracer compounds as a second plurality of specific binding partners, for the specific determination of analytes out of the first plurality of specific binding partners contained in the samples, are brought into contact with the samples deposited in said discrete measurement areas in a single step or multiple steps of a specific binding reaction, changes in opto-electronic signals, resulting from the binding of tracer compounds to analytes contained in the samples in discrete measurement areas or to specific binding partners bound to the analytes contained in the measurement areas in the evanescent field of the evanescent field sensor platform are measured laterally resolved, and the presence of the analytes to be specifically detected is determined qualitatively and/or quantitatively from the relative amount of the changes in said opto-electronic signals from the corresponding measurement areas.

摘要翻译： 本发明涉及一种分析平台及其分析方法，用于分析大量与分析物相似的“自然相同”的样品，其中所含的分析物样品在其中包含并具有生物相关性，作为特异性结合反应中的结合配偶体，其中所述样品或 与原始样品相同的相对分子组成的所述样品与其中包含的待测定的分析物作为第一多个特异性结合配偶体的稀释沉积在至少一个一维或二维阵列的离散测量区域中 相对于样品的原始相对分子组成，作为第二多个特异性结合配偶体的一个或多个示踪剂化合物，作为固体支持物的ev逝场传感器平台上的测量区域作​​为固体支持物而没有相对分子组成的变化 确定包含在第一个多个具体约束伙伴中的分析物 在与单独的步骤或特定结合反应的多个步骤中，沉积在所述离散测量区域中的样品与示踪剂化合物与样品中包含的分析物的离散性的光电子信号的变化相接触 测量区域或与消逝场传感器平台的消逝场中测量区域中包含的分析物结合的特异性结合配体进行横向分辨测定，并且特异性检测的分析物的存在是从定性和/或定量的 相应测量区域的所述光电信号的相对变化量。

公开(公告)号：US20080020409A1

公开(公告)日：2008-01-24

申请号：US10598418

申请日：2004-03-03

申请人： Michael Pawlak ,  Eginhard Schick ,  Peter Oroszlan

发明人： Michael Pawlak ,  Eginhard Schick ,  Peter Oroszlan

IPC分类号： G01N33/574 ,  C12M1/34 ,  C40B30/04 ,  G01N33/567 ,  G01N33/569

CPC分类号： G01N33/6845 ,  G01N33/567 ,  G01N33/6842

摘要： The present invention is related to analytical platforms and methods performed therewith for generating qualitative and/or quantitative protein expression profiles, in particular differential protein expression profiles, of cell populations comprising: generating lysates of one or more populations of cells, the lysates comprising a plurality of proteins expressed by the respective cell populations, providing an essentially planar solid support, depositing at discrete sites small quantities of the cell lysates, in diluted or undiluted form directly on said solid support or on an adhesion-promoting layer applied on said solid support, thereby creating one or more one- or two-dimensional arrays of discrete measurement areas on said solid support, applying a number of binding reagents as specific binding partners for the proteins contained in cell lysates in discrete measurement areas and to be detected and, if adequate, one or more detection reagents on said one or more arrays of measurement areas, the binding reagents and the detection reagents being applied sequentially or in a single addition-step, after binding of the detection reagents to the binding reagents, to the one or more arrays of discrete measurement areas for e.g. global analysis of signaling pathways or screening antibody sets/libraries against protein targets for best specificity, selectivity and affinity, and measuring and recording optical signals emanating from said one or more arrays of discrete measurement areas in a locally resolved manner, wherein said essentially planar solid support is non-porous and an optionally applied adhesion-promoting layer has a thickness of less than 1 μm.

摘要翻译： 本发明涉及用于产生细胞群体的定性和/或定量蛋白质表达谱，特别是差异蛋白质表达谱的分析平台和方法，其包括：产生一个或多个细胞群的裂解物，裂解物包含多个 由相应的细胞群体表达的蛋白质，提供基本上平面的固体支持物，在稀释或未稀释的形式的离散位置沉积在所述固体支持物上或施加在所述固体支持物上的粘附促进层上， 从而在所述固体支持物上产生离散测量区域的一个或多个一维或二维阵列，将多个结合试剂作为离散测量区域中包含在细胞裂解物中的蛋白质的特异性结合配偶体，并且如果足够 ，所述一个或多个测量阵列上的一种或多种检测试剂 在将检测试剂与结合试剂结合后，顺序地或在单个添加步骤中将结合试剂和检测试剂施用到例如离子测量区域的一个或多个阵列。 全面分析信号通路或筛选针对蛋白质靶标的抗体集/文库，以获得最佳特异性，选择性和亲和力，以及以局部分辨方式测量和记录从所述一个或多个离散测量区域阵列发出的光信号，其中所述基本上平面的固体 支撑体是无孔的，并且任选地施加的粘附促进层的厚度小于1μm。

公开(公告)号：US20090311773A1

公开(公告)日：2009-12-17

申请号：US11919054

申请日：2006-04-22

申请人： Eginhard Schick ,  Dominic Utinger ,  Claudio Calonder

发明人： Eginhard Schick ,  Dominic Utinger ,  Claudio Calonder

IPC分类号： C12M1/34 ,  B05B1/00 ,  B05D1/02

CPC分类号： B05B17/0615 ,  B01L3/00

摘要： The invention relates to several embodiments of equipment for coating substrates for detecting one or more analytes by way of an affinity assay method, comprising: a receptacle receiving a liquid to be atomized (“liquid receptacle”) with substances (compounds) to be deposited onto at least one surface of said substrates and an atomized volume produced by the liquid in the operating state; an actuator for triggering the atomization process and; a fixture for receiving and storing the substrates during the coating process. The invention is characterized in that the substrates are not in contact with the surface of the liquid to be atomized. The invention also relates to several embodiments of methods for coating substrates with coupler and/or passivation layers for use in the detection of one or more analytes by way of an affinity assay method.

摘要翻译： 本发明涉及用于通过亲和测定方法检测一种或多种分析物的基质用于涂覆基底的设备的几个实施方案，包括：容纳要被雾化的液体（“液体容器”）与要沉积的物质（化合物）的容器 所述衬底的至少一个表面和在操作状态下由液体产生的雾化体积; 用于触发雾化过程的致动器; 用于在涂覆过程中接收和存储基底的夹具。 本发明的特征在于，基板不与待雾化的液体的表面接触。 本发明还涉及用于用用于通过亲和测定方法检测一种或多种分析物的成色剂和/或钝化层涂覆基质的方法的几个实施方案。

公开(公告)号：US09050615B2

公开(公告)日：2015-06-09

申请号：US11919054

申请日：2006-04-22

申请人： Eginhard Schick ,  Dominic Utinger ,  Claudio Calonder

发明人： Eginhard Schick ,  Dominic Utinger ,  Claudio Calonder

IPC分类号： B01L3/00 ,  B05B17/06

CPC分类号： B05B17/0615 ,  B01L3/00

摘要： The invention relates to several embodiments of equipment for coating substrates for detecting one or more analytes by way of an affinity assay method, comprising: a receptacle receiving a liquid to be atomized (“liquid receptacle”) with substances (compounds) to be deposited onto at least one surface of said substrates and an atomized volume produced by the liquid in the operating state; an actuator for triggering the atomization process and; a fixture for receiving and storing the substrates during the coating process. The invention is characterized in that the substrates are not in contact with the surface of the liquid to be atomized. The invention also relates to several embodiments of methods for coating substrates with coupler and/or passivation layers for use in the detection of one or more analytes by way of an affinity assay method.

摘要翻译： 本发明涉及用于通过亲和测定方法检测一种或多种分析物的基质用于涂覆基底的设备的几个实施方案，包括：容纳要被雾化的液体（“液体容器”）与要沉积的物质（化合物）的容器 所述衬底的至少一个表面和在操作状态下由液体产生的雾化体积; 用于触发雾化过程的致动器; 用于在涂覆过程中接收和存储基底的夹具。 本发明的特征在于，基板不与待雾化的液体的表面接触。 本发明还涉及用于用用于通过亲和测定方法检测一种或多种分析物的成色剂和/或钝化层涂覆基质的方法的几个实施方案。