公开(公告)号：US20220064683A1

公开(公告)日：2022-03-03

申请号：US17464139

申请日：2021-09-01

Applicant: Braskem S.A.

Inventor： Iuri Estrada GOUVEA ,  Marcos Rogerio Simoes ,  Mariana Trovo Marchesin ,  Aline Silva Romao Dumaresq

IPC: C12P17/04 ,  C12N9/12 ,  C12N9/88 ,  C12N9/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04

Abstract: The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandicarboxylic acid from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandicarboxylic acid with ethanol and for co-producing 2,4-furandicarboxylic acid with ethanol and 1-propanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandicarboxylic acid and that coupled the 2,4-furandicarboxylic acid pathway with an additional metabolic pathway.

公开(公告)号：US20190323016A1

公开(公告)日：2019-10-24

申请号：US16317406

申请日：2017-07-12

Applicant: BRASKEM S.A.

Inventor： Mateus Schreiner LOPES ,  Daniel Johannes KOCH ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Veronica Leite QUEIROZ

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/04

Abstract: The present application relates to recombinant microorganisms expressing a dehydratase useful in a one-step, direct fermentative production of one or more primary alkenes from one or more saturated primary or secondary alcohols. Known, well developed high-yielding pathways that use renewable feedstock can be introduced into the recombinant microorganisms to obtain the alcohol precursors. Also provided are methods of producing one or more primary alkenes using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the primary alkene products.