公开(公告)号：US20190309266A1

公开(公告)日：2019-10-10

申请号：US16378209

申请日：2019-04-08

Applicant: Braskem S.A.

Inventor： Verônica Leite QUEIROZ ,  Lucas Pedersen PARIZZI ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Rafael Victório Carvalho GUIDO ,  Alessandro Silva NASCIMENTO ,  Igor POLIKARPOV

IPC: C12N9/04 ,  C12N15/52

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US20190323016A1

公开(公告)日：2019-10-24

申请号：US16317406

申请日：2017-07-12

Applicant: BRASKEM S.A.

Inventor： Mateus Schreiner LOPES ,  Daniel Johannes KOCH ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Veronica Leite QUEIROZ

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/04

Abstract: The present application relates to recombinant microorganisms expressing a dehydratase useful in a one-step, direct fermentative production of one or more primary alkenes from one or more saturated primary or secondary alcohols. Known, well developed high-yielding pathways that use renewable feedstock can be introduced into the recombinant microorganisms to obtain the alcohol precursors. Also provided are methods of producing one or more primary alkenes using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the primary alkene products.

公开(公告)号：US20230279365A1

公开(公告)日：2023-09-07

申请号：US18084888

申请日：2022-12-20

Applicant: Braskem S.A.

Inventor： Verônica Leite QUEIROZ ,  Lucas Pedersen PARIZZI ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Rafael Victório Carvalho GUIDO ,  Alessandro Silva NASCIMENTO ,  Igor POLIKARPOV

IPC: C12N9/04 ,  C12N15/52

CPC classification number: C12N9/0006 ,  C12N15/52 ,  C12Y101/01001

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.