公开(公告)号：US20220298535A1

公开(公告)日：2022-09-22

申请号：US17750293

申请日：2022-05-20

Applicant: Braskem S.A.

Inventor： Paulo Moises Raduan ALEXANDRINO ,  Iuri Estrada GOUVEA ,  Veronica Leite QUEIROZ

IPC: C12P17/04 ,  C07D307/68 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/08 ,  C12N9/16

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20220064682A1

公开(公告)日：2022-03-03

申请号：US17463988

申请日：2021-09-01

Applicant: Braskem S.A.

Inventor： Iuri Estrada GOUVEA ,  Ana Karina BRAMBILLA COSTA ,  Marcos Rogerio SIMOES ,  Veronica Leite QUEIROZ ,  Aline Silva ROMÃO DUMARESQ

IPC: C12P17/04 ,  C12N1/16 ,  C12N15/81

Abstract: The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandimethanol from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandimethanol with ethanol and for co-producing 2,4-furandimethanol with ethanol and acetone and/or isopropanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandimethanol and that couple the 2,4-furandimethanol pathway with an additional metabolic pathway. The present disclosure further provides enzymatic production of 2,4-furandicarboxylic acid.

公开(公告)号：US20230279365A1

公开(公告)日：2023-09-07

申请号：US18084888

申请日：2022-12-20

Applicant: Braskem S.A.

Inventor： Verônica Leite QUEIROZ ,  Lucas Pedersen PARIZZI ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Rafael Victório Carvalho GUIDO ,  Alessandro Silva NASCIMENTO ,  Igor POLIKARPOV

IPC: C12N9/04 ,  C12N15/52

CPC classification number: C12N9/0006 ,  C12N15/52 ,  C12Y101/01001

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US20210238639A1

公开(公告)日：2021-08-05

申请号：US17219792

申请日：2021-03-31

Applicant: Braskem S.A.

Inventor： Paulo Moises Raduan ALEXANDRINO ,  Iuri Estrada GOUVEA ,  Veronica Leite QUEIROZ ,  Felipe Cicaroni FERNANDES ,  Bárbara MANO

IPC: C12P17/04 ,  C07D307/68

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20220243006A1

公开(公告)日：2022-08-04

申请号：US17586535

申请日：2022-01-27

Applicant: Braskem S.A.

Inventor： Felipe Cicaroni FERNANDES ,  Iuri Estrada GOUVEA ,  Bárbara MANO

IPC: C08G63/183 ,  C08G63/40

Abstract: The present disclosure provides a copolyester including a dicarboxylic acid component A comprising a terephthalic acid residue or ester-forming derivative thereof, or a mixture thereof, and a 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, or a mixture thereof, and a diol component B comprising an alkanediol residue having from 2 to 22 carbon atoms, wherein the dicarboxylic acid component A has a total molar content, and wherein the 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, is present in an amount of from 0.1 to 10 mol %, with respect to the total molar content of the dicarboxylic acid component A. Inventive copolyesters have a slower crystallization rate, a higher gas barrier to CO2 and O2 and a higher ratio of 14C to 12C as measured by ASTM D6866 when compared to a comparable copolyester comprising isophthalic acid instead of 2,4-furandicarboxylic acid.

公开(公告)号：US20210222217A1

公开(公告)日：2021-07-22

申请号：US17163838

申请日：2021-02-01

Applicant: Braskem S.A.

Inventor： Paulo Moises Raduan ALEXANDRINO ,  Iuri Estrada GOUVEA ,  Veronica Leite QUEIROZ ,  Marcos Rogerio SIMÕES

IPC: C12P17/04 ,  C07D307/68

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-IMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20200277639A1

公开(公告)日：2020-09-03

申请号：US16806728

申请日：2020-03-02

Applicant: Braskem S.A.

Inventor： Paulo Moises Raduan ALEXANDRINO ,  Iuri Estrada GOUVEA ,  Veronica Leite QUEIROZ

IPC: C12P17/04 ,  C07D307/68

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20190309266A1

公开(公告)日：2019-10-10

申请号：US16378209

申请日：2019-04-08

Applicant: Braskem S.A.

Inventor： Verônica Leite QUEIROZ ,  Lucas Pedersen PARIZZI ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Rafael Victório Carvalho GUIDO ,  Alessandro Silva NASCIMENTO ,  Igor POLIKARPOV

IPC: C12N9/04 ,  C12N15/52

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US20220090153A1

公开(公告)日：2022-03-24

申请号：US17545616

申请日：2021-12-08

Applicant: Braskem S.A.

Inventor： Iuri Estrada GOUVEA ,  Paulo Moises Raduan ALEXANDRINO

IPC: C12P17/04 ,  C07D307/68

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20220064683A1

公开(公告)日：2022-03-03

申请号：US17464139

申请日：2021-09-01

Applicant: Braskem S.A.

Inventor： Iuri Estrada GOUVEA ,  Marcos Rogerio Simoes ,  Mariana Trovo Marchesin ,  Aline Silva Romao Dumaresq

IPC: C12P17/04 ,  C12N9/12 ,  C12N9/88 ,  C12N9/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04

Abstract: The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandicarboxylic acid from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandicarboxylic acid with ethanol and for co-producing 2,4-furandicarboxylic acid with ethanol and 1-propanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandicarboxylic acid and that coupled the 2,4-furandicarboxylic acid pathway with an additional metabolic pathway.