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公开(公告)号：US07195871B2
公开(公告)日：2007-03-27
申请号：US10290386
申请日：2002-11-07
申请人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew A. Lukowiak
发明人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew A. Lukowiak
CPC分类号： C12Q1/6827 , A61K38/00 , C07K2319/02 , C12N9/1252 , C12N9/22 , C12Q2561/109 , C12Q2525/125 , C12Q2525/179 , C12Q2537/143
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US08445238B2
公开(公告)日：2013-05-21
申请号：US11866663
申请日：2007-10-03
IPC分类号： C12P19/34
CPC分类号： C12Q1/6858 , C12Q1/6813 , C12Q1/6827 , C12Q2525/301 , C12Q2561/109
摘要： The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3′ hairpin structures, 5′ hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5′ or 3′ ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.
摘要翻译： 本发明提供了包含用于形成3'发夹结构，5'发夹结构和双发夹结构的快速反应引物的方法，组合物和试剂盒。 发夹结构可用于检测目标序列（例如，例如小RNA靶序列），用于检测靶序列中的多态性（例如，位于靶序列的5'或3'末端附近的多态性）或其他 核酸表征方法。 在某些实施方案中，发夹结构形成侵入性切割结构（例如，与探针或上游寡核苷酸组合），其可被结构特异性酶切割以检测特定核苷酸或核苷酸序列的存在或不存在。
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公开(公告)号：US20090142752A1
公开(公告)日：2009-06-04
申请号：US11866663
申请日：2007-10-03
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6813 , C12Q1/6827 , C12Q2525/301 , C12Q2561/109
摘要： The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3′ hairpin structures, 5′ hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5′ or 3′ ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.
摘要翻译： 本发明提供了包含用于形成3'发夹结构，5'发夹结构和双发夹结构的快速反应引物的方法，组合物和试剂盒。 发夹结构可用于检测目标序列（例如，例如小RNA靶序列），用于检测靶序列中的多态性（例如，位于靶序列的5'或3'末端附近的多态性）或其他 核酸表征方法。 在某些实施方案中，发夹结构形成侵入性切割结构（例如，与探针或上游寡核苷酸组合），其可被结构特异性酶切割以检测特定核苷酸或核苷酸序列的存在或不存在。
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公开(公告)号：US07790393B2
公开(公告)日：2010-09-07
申请号：US12174277
申请日：2008-07-16
CPC分类号： G06Q30/06 , G06Q10/087
摘要： The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions.
摘要翻译： 本发明提供用于开发和优化用于基础研究，临床研究和用于开发临床检测测定的核酸检测测定的方法和程序。 特别地，本发明提供了用于多重扩增反应中使用的寡核苷酸引物的设计方法。 本发明还提供优化多重扩增反应的方法。
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