公开(公告)号：US20240117424A1

公开(公告)日：2024-04-11

申请号：US18367308

申请日：2023-09-12

Applicant: Illumina, Inc.

Inventor： Jonathan Boutell ,  Wayne George ,  Xiaolin Wu

IPC: C12Q1/6874 ,  C07D207/46 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C07D207/46 ,  C12Q1/6806

Abstract: Reusable flow cells for sequencing which exhibit signal intensity retention over numerous use cycles, the active surface of which contains poly-azide functional moieties, methods of treating flow cells surfaces with reagents to provide such poly-azide functional moieties, and reagents therefor.

公开(公告)号：US20180187252A1

公开(公告)日：2018-07-05

申请号：US15844051

申请日：2017-12-15

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Shaun Hunter ,  Peter McInerney ,  Jonathan Boutell ,  Claire Bevis-Mott

IPC: C12Q1/6848 ,  B01J19/00 ,  C12Q1/6874 ,  B01L3/00

Abstract: An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.