公开(公告)号：US10767161B2

公开(公告)日：2020-09-08

申请号：US14928383

申请日：2015-10-30

Applicant: Indiana University Research and Technology Corporation

Inventor： Mervin C. Yoder ,  David A. Ingram

IPC: C12N5/00 ,  C12N5/0789 ,  C12N5/071 ,  G01N33/50 ,  G01N33/569 ,  A61K35/12

Abstract: A hierarchy of endothelial colony forming cells (EPCs) was identified from mammalian cord blood, umbilical vein and aorta. A newly isolated cell named high proliferative potential—endothelial colony forming cell (HPP-ECFC) was isolated and characterized. Single cell assays were developed that test the proliferative and clonogenic potential of endothelial cells derived from cord blood, or from HUVECs and HAECs. EPCs were found to reside in vessel walls. Use of a feeder layer of cells derived from high proliferative potential-endothelial colony forming cells (HPP-ECPCS) from human umbilical cord blood, stimulates growth and survival of repopulating hematopoietic stem and progenitor cells. Stimulation of growth and survival was determined by increased numbers of progenitor cells in in vitro cultures and increased levels of human cell engraftment in the NOD/SCID immunodeficient mouse transplant system.

公开(公告)号：US20200017835A1

公开(公告)日：2020-01-16

申请号：US16509902

申请日：2019-07-12

Applicant: Indiana University Research and Technology Corporation

Inventor： Mervin C. Yoder ,  Yang Lin

IPC: C12N5/071 ,  C07K16/28 ,  A61K35/44 ,  A61P9/10

Abstract: Methods are provided for the isolation, expansion, enrichment, and transplantation of endothelial stem cells from blood vessels and induced pluripotent stem cells via the use of ABCG2 cell surface marker. The ability of the endothelial stem cells to expand in vitro and be subsequently implanted in vivo to generate new blood vessels provides a therapeutic hope for patients with numerous cardiovascular disorders (peripheral arterial disease, critical limb ischemia, ischemic retinopathies, acute ischemic injury to kidney, and myocardial infarction) where the lack of sufficient blood vessel forming ability in the patient limits their regenerative capacity.