公开(公告)号：US08765641B2

公开(公告)日：2014-07-01

申请号：US10593412

申请日：2005-03-18

申请人： Hyesook Kim ,  Alan A. Dombkowski ,  Raymond F. Novak ,  Brian B. Haab

发明人： Hyesook Kim ,  Alan A. Dombkowski ,  Raymond F. Novak ,  Brian B. Haab

IPC分类号： C40B30/04 ,  G01N33/563

CPC分类号： G01N33/54366 ,  G01N33/54306 ,  G01N33/573 ,  G01N2333/90245

摘要： An antibody microarray screen including a substrate, monoclonal and polyclonal antibodies that are purified immunoglobins, wherein the antibodies are spotted on predetermined positions on the substrate, and fluids unprocessed for immunoglobulin isolation (e.g., anti-sera, ascites fluids, or hybridoma culture media), wherein the unprocessed fluids are spotted on the predetermined positions on the substrate. Production of drug-metabolizing enzyme antibody microarrays containing closely related cytochromes P450 is disclosed. Methods of manufacturing an antibody microarray, an internal control molecule for use in an antibody microarray, a method of determining optimal spotting concentrations of IgG and a method to increase a detectable signal with microarray analysis are disclosed.

摘要翻译： 一种包含底物，纯化免疫球蛋白的单克隆抗体和多克隆抗体的抗体微阵列筛选，其中所述抗体被点在基底上的预定位置，以及未加工用于免疫球蛋白分离的流体（例如抗血清，腹水或杂交瘤培养基） ，其中未处理的流体被点在基板上的预定位置上。 公开了含有密切相关的细胞色素P450的药物代谢酶抗体微阵列的生产。 公开了制造抗体微阵列的方法，用于抗体微阵列的内部对照分子，确定IgG的最佳点样浓度的方法以及通过微阵列分析增加可检测信号的方法。

公开(公告)号：US20110262444A1

公开(公告)日：2011-10-27

申请号：US13039894

申请日：2011-03-03

申请人： Hyesook Kim

发明人： Hyesook Kim

IPC分类号： A61K39/395 ,  A61P29/00 ,  G01N33/574 ,  A61P35/00 ,  C07K1/00 ,  C07K1/14

CPC分类号： C07K16/22 ,  C07K16/3069 ,  C07K2317/10 ,  C07K2317/33 ,  C07K2317/34 ,  G01N33/574 ,  G01N33/57407 ,  G01N33/57488 ,  G01N2333/495 ,  G01N2333/96433

摘要： A method of producing form-specific anti-peptide antibodies for a wild type protein and its one amino acid mutated protein using a peptide antigen, by obtaining a protein sequence of the wild type protein and its one amino acid mutated protein, selecting a continuous amino acid sequence without any internal cysteine residues that includes the one amino acid mutated sequence and wild type sequence corresponding to the mutated site at the end of the sequence to obtain a synthetic mutation peptide and a synthetic wild type peptide, conjugating the synthetic peptides to a carrier protein, and immunizing an animal to produce antibodies. Methods of detecting cancer and methods of treating cancer.

摘要翻译： 通过获得野生型蛋白质及其一种氨基酸突变蛋白质的蛋白质序列，使用肽抗原制备野生型蛋白质及其一种氨基酸突变蛋白质的形式特异性抗肽抗体的方法，选择连续氨基酸 没有任何内部半胱氨酸残基的酸序列，其包括对应于序列末端的突变位点的一个氨基酸突变序列和野生型序列，以获得合成突变肽和合成的野生型肽，将合成肽与载体 蛋白质，并免疫动物产生抗体。 检测癌症的方法和治疗癌症的方法。

公开(公告)号：US20070207503A1

公开(公告)日：2007-09-06

申请号：US11681243

申请日：2007-03-02

申请人： Hyesook Kim ,  Elizabeth Roberts-Kirchoff

发明人： Hyesook Kim ,  Elizabeth Roberts-Kirchoff

IPC分类号： G01N33/53 ,  C12P21/04 ,  C12P7/64

CPC分类号： G01N33/92 ,  G01N33/98

摘要： A method of producing an antibody recognizing a target fatty acid alcohol ester by immunizing an animal with a carrier protein conjugated with a derivative of the target fatty acid alcohol ester. A method of estimating alcohol consumption of an individual by quantitating levels of molecules which bind to antibodies produced with a derivative of a target fatty acid alcohol ester conjugated to a carrier protein in a biological sample obtained from the individual. A kit for estimating alcohol consumption of an individual including means for quantitating levels of molecules which bind to antibodies produced with a derivative of a target fatty acid alcohol ester conjugated to a carrier protein in a biological sample obtained from the individual.

摘要翻译： 通过用与目标脂肪酸醇酯的衍生物结合的载体蛋白免疫动物来产生识别目标脂肪酸醇酯的抗体的方法。 通过定量结合由从个体获得的生物样品中与载体蛋白缀合的靶脂肪酸醇酯的衍生物产生的抗体的分子水平来估计个体的饮酒量的方法。 用于估计个体的酒精消耗的试剂盒，包括用于定量结合由从个体获得的生物样品中与载体蛋白缀合的目标脂肪酸醇酯的衍生物产生的抗体的分子水平的手段。

公开(公告)号：US20060051787A1

公开(公告)日：2006-03-09

申请号：US11169080

申请日：2005-06-28

申请人： Hyesook Kim

发明人： Hyesook Kim

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12Q1/6883

摘要： A method to assess DNA damage using quantitative polymerase chain reaction (QPCR) with an internal control with or without modified QPCR primers is disclosed. The method further included quantitation of QPCR products using a chemiluminescent, colorimetric or fluorescent assay and a real-time PCR. The chemiluminescent, colorimetric or fluorescent assay is carried out with modified PCR primers and the real-time PCR is carried out with PCR primers with or without modification. A method to select a suitable PCR replication cycle number is disclosed. This is to avoid polymerization of PCR products after addition of the internal control. A method to analyze DNA damage and mutagenicity induced by chemicals in DNA damage repair-deficient and proficient fibroblast cells with endogenous expression of oxidative stress-inducing proteins, e.g., prostaglandin H2 synthase form 2 (PGHS-2, also called as COX-2), was disclosed.

摘要翻译： 公开了使用具有或不具有修饰的QPCR引物的内部对照的定量聚合酶链反应（QPCR）来评估DNA损伤的方法。 该方法还包括使用化学发光，比色或荧光测定和实时PCR定量QPCR产物。 化学发光，比色或荧光测定用修饰的PCR引物进行，并且使用具有或不具有修饰的PCR引物进行实时PCR。 公开了选择合适的PCR复制循环数的方法。 这是为了避免PCR产物在加入内部对照后的聚合。 一种分析DNA损伤和致突变性的方法，其中DNA损伤修复缺陷型和熟练的成纤维细胞具有内源性表达的氧化应激诱导蛋白，例如前列腺素H 2合成酶2（PGHS-2 ，也称为COX-2）。

公开(公告)号：US06812212B2

公开(公告)日：2004-11-02

申请号：US09915776

申请日：2001-07-26

申请人： Hyesook Kim ,  Elizabeth Starr Roberts-Kirchhoff

发明人： Hyesook Kim ,  Elizabeth Starr Roberts-Kirchhoff

IPC分类号： A61K3800

CPC分类号： G01N33/5091 ,  G01N33/68 ,  G01N2333/99

摘要： There is provided a method of assessing oxidant stress by measuring polymerization of proteins. Also provided is a marker for oxidant stress which includes a polymerized protein. A kit for use in assessing oxidant stress, the kit including an assay for detecting polymerized proteins is also provided. A method of lowering oxidant stress by administering to a patient an effective amount of at least one reducing agent is also provided. A pharmaceutical composition for lowering oxidant stress, the pharmaceutical having an effective amount of reducing agent and a pharmaceutically acceptable carrier is also provided.

摘要翻译： 提供了一种通过测量蛋白质聚合来评估氧化应激的方法。 还提供了包含聚合蛋白质的氧化应激标记物。 还提供了用于评估氧化应激的试剂盒，该试剂盒包括用于检测聚合蛋白质的测定法。 还提供了通过向患者施用有效量的至少一种还原剂来降低氧化应激的方法。 还提供了用于降低氧化应激的药物组合物，具有有效量的还原剂和药学上可接受的载体的药物。

公开(公告)号：US20220276265A9

公开(公告)日：2022-09-01

申请号：US16259499

申请日：2019-01-28

申请人： Hyesook Kim

发明人： Hyesook Kim

IPC分类号： G01N33/92 ,  G01N33/68 ,  G01N33/573

摘要： This invention discloses diagnosis of risk of chemotherapy-induced cardiotoxicity by measurement of increased expression of soluble epoxide hydrolase in vitro and in vivo in cells, tissues or animals including measurement of increased levels of soluble epoxide hydrolase metabolites, e.g., 14,15-DHET and 11,12-DHET, in biological fluids. This invention also includes diagnosis of risk of chemotherapy-induced cardiotoxicity by measuring increased levels of oxidative stress in cells, tissues or animals including measurement of increased levels of oxidative stress biomarkers, e.g., 8-isoprostane, in biological fluids. Fatty acid and protein biomarkers to diagnose the risk of chemotherapy-induced cardiotoxicity are detected using various detection methods including mass spectrometry and immunoassay such as ELISA, Western blot analysis or label-free microwell and nanowell technologies. This invention discloses targeted medical intervention for a subject who is at risk or with chemotherapy-induced cardiotoxicity by treating with soluble epoxide hydrolase inhibitor(s) with or without antioxidants to prevent or ameliorate the chemotherapy-induced cardiotoxicity.

公开(公告)号：US20200378950A1

公开(公告)日：2020-12-03

申请号：US16423060

申请日：2019-05-27

申请人： Hyesook Kim ,  So Hee Kim

发明人： Hyesook Kim ,  So Hee Kim

IPC分类号： G01N33/50 ,  C07K16/32

摘要： A method of characterizing the protein O-GlcNAcylation site-specificity of an antibody. A method of detecting or quantifying the expression of site-specific O-GlcNAcylated proteins expressed in cells and biological samples. A method of diagnosing cancer in a host based on the cellular expression of site-specific O-GlcNAcylated proteins. A method of screening anti-cancer compounds according to their ability to increase a level O-GlcNAcylation of oncogene or tumor suppressor proteins. Methods of treating cancer in an animal host by administering compounds that increase a level of O-GlcNAcylated c-myc or p53 in cancer cells. A method of distinguishing subclasses of pancreatic cancer according to the sensitivity of pancreatic cancer cells to an imidazole derivative, and a method of personalized pancreatic cancer treatment delivered according to the sensitivity subclasses.

公开(公告)号：US09212221B2

公开(公告)日：2015-12-15

申请号：US13039894

申请日：2011-03-03

申请人： Hyesook Kim

发明人： Hyesook Kim

IPC分类号： C07K1/00 ,  C07K16/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/28 ,  C07K16/30 ,  A61K6/00 ,  A61K47/00 ,  G01N33/574

CPC分类号： C07K16/22 ,  C07K16/3069 ,  C07K2317/10 ,  C07K2317/33 ,  C07K2317/34 ,  G01N33/574 ,  G01N33/57407 ,  G01N33/57488 ,  G01N2333/495 ,  G01N2333/96433

摘要： A method of producing form-specific anti-peptide antibodies for a wild type protein and its one amino acid mutated protein using a peptide antigen, by obtaining a protein sequence of the wild type protein and its one amino acid mutated protein, selecting a continuous amino acid sequence without any internal cysteine residues that includes the one amino acid mutated sequence and wild type sequence corresponding to the mutated site at the end of the sequence to obtain a synthetic mutation peptide and a synthetic wild type peptide, conjugating the synthetic peptides to a carrier protein, and immunizing an animal to produce antibodies. Methods of detecting cancer and methods of treating cancer.

摘要翻译： 通过获得野生型蛋白质及其一种氨基酸突变蛋白质的蛋白质序列，使用肽抗原制备野生型蛋白质及其一种氨基酸突变蛋白质的形式特异性抗肽抗体的方法，选择连续氨基酸 没有任何内部半胱氨酸残基的酸序列，其包括对应于序列末端的突变位点的一个氨基酸突变序列和野生型序列，以获得合成突变肽和合成的野生型肽，将合成肽与载体 蛋白质，并免疫动物产生抗体。 检测癌症的方法和治疗癌症的方法。

公开(公告)号：US20100159481A1

公开(公告)日：2010-06-24

申请号：US12716570

申请日：2010-03-03

申请人： Hyesook Kim ,  Jorge H. Capdevila ,  Raymond F. Novak ,  Deanna Kroetz

发明人： Hyesook Kim ,  Jorge H. Capdevila ,  Raymond F. Novak ,  Deanna Kroetz

IPC分类号： G01N33/573 ,  C12Q1/34

CPC分类号： G01N33/88 ,  G01N2800/321

摘要： A method of assessing arachidonic acid (AA) metabolites-dependent hypertension by measuring glucuronidated dihydroxyeicosatrienoic acids (DHETs) and DHET metabolites in a biological sample which contains the epitopes unique to DHET (using any methods including GC/MS, LC/MS or ELISA). An example of the glucuronidated DHET metabolite is DHET-alcohols such as omega or omega-1 oxidated DHET and DHET esterified glycerol. The method further includes determining the amount of glucuronidated molecules containing a DHET-specific epitope which is immunoreactive with antibodies produced against DHETs.

摘要翻译： 通过测量包含DHET特有的表位的生物样品中的葡糖醛酸二羟基二十碳三烯酸（DHET）和DHET代谢物来评估花生四烯酸（AA）代谢物依赖性高血压的方法（使用任何方法，包括GC / MS，LC / MS或ELISA） 。 葡萄糖醛酸化的DHET代谢物的一个实例是DHET-醇如ω或ω-1氧化的DHET和DHET酯化甘油。 该方法还包括确定含有针对DHET产生的抗体具有免疫反应性的DHET特异性表位的葡糖醛酸化分子的量。

公开(公告)号：US20080145858A1

公开(公告)日：2008-06-19

申请号：US11964117

申请日：2007-12-26

申请人： Hyesook Kim ,  Mary Murray ,  Raymond F. Novak

发明人： Hyesook Kim ,  Mary Murray ,  Raymond F. Novak

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  A01K2227/50 ,  C12Q2600/142 ,  C12Q2600/158

摘要： A screen for detecting, identifying, and characterizing chemicals as toxicants is based on the affect of the chemical on gene expression in animal cleavage stage embryos. A microarray screen for detecting and measuring the affects of chemicals on gene expression in animal cleavage stage embryos. A screen for detecting, identifying and characterizing chemicals as toxicants based on the common or differential affects on gene expression in animal cleavage stage embryos and neurulation stage embryos. Markers of chemical exposure and teratogenesis identified using the screen disclosed herein. A treatment that enables the transfer of biotinylated PCR products or DNA to a membrane following gel electrophoresis by depurinating the PCR or DNA products and denaturing the PCR products or DNA.

摘要翻译： 用于检测，鉴定和表征化学品作为有毒物质的屏幕是基于化学物质对动物切割阶段胚胎中基因表达的影响。 一种用于检测和测量化学物质对动物切割期胚胎中基因表达的影响的微阵列筛选。 基于对动物切割阶段胚胎和神经阶段胚胎中基因表达的共同或不同影响的用于检测，鉴定和表征化学品作为有毒物质的筛选。 使用本文公开的屏幕鉴定化学暴露和致畸的标记。 通过对PCR或DNA产物进行降解和变性PCR产物或DNA，可以在凝胶电泳后将生物素化的PCR产物或DNA转移到膜上进行处理。