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    Plant Activation of Insect Toxin
    11.
    发明申请
    Plant Activation of Insect Toxin 有权
    植物激活昆虫毒素

    公开(公告)号：US20080200415A1

    公开(公告)日：2008-08-21

    申请号：US11940002

    申请日：2007-11-14

    申请人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    IPC分类号： A61K31/7088 ,  C12N15/32 ,  A01P7/04 ,  A01H5/00

    CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

    PLANT ACTIVATION OF INSECT TOXIN
    12.
    发明申请
    PLANT ACTIVATION OF INSECT TOXIN 失效
    植物激活的毒素

    公开(公告)号：US20080124799A1

    公开(公告)日：2008-05-29

    申请号：US11949912

    申请日：2007-12-04

    申请人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    IPC分类号： C12N5/04 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

    Isolated nucleic acid molecules encoding orally active androctonus amoreuxi pesticidal biopeptides
    19.
    发明授权
    Isolated nucleic acid molecules encoding orally active androctonus amoreuxi pesticidal biopeptides 有权
    分离编码口服活性的芦荟杀虫生物肽的核酸分子

    公开(公告)号：US07414173B1

    公开(公告)日：2008-08-19

    申请号：US10617978

    申请日：2003-07-11

    申请人： Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  James F. H. Wong

    发明人： Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  James F. H. Wong

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/12 ,  C07K14/435

    CPC分类号： C12N15/8286 ,  C07K14/43522 ,  Y02A40/162

    摘要： The present invention provides compositions and methods for isolated nucleic acid molecules encoding orally active Androctonus amoreuxi pesticidal polypeptides. Compositions include novel nucleotide sequences encoding Androctonus amoreuxi pesticidal polypeptides, and biologically active variants thereof. Further provided are methods for modulating the pesticide resistance of plants using the nucleotide sequences disclosed herein. One method comprises stably transforming into the genome of a plant cell a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. An additional method comprises incorporating a nucleotide sequence of the present invention operably linked to a heterologous promoter into a microorganism and applying said microorganism to the environment of a plant.

    摘要翻译： 本发明提供了编码口服活性黄芪杀虫多肽的分离的核酸分子的组合物和方法。 组合物包括编码Androctonus amoreuxi杀虫多肽的新型核苷酸序列及其生物活性变体。 还提供了使用本文公开的核苷酸序列调节植物的农药抗性的方法。 一种方法包括稳定地转化到植物细胞的基因组中，本发明的核苷酸序列与异源启动子可操作地连接并再生表达核苷酸序列的稳定转化的植物。 另外的方法包括将可操作地连接到异源启动子的本发明的核苷酸序列并入微生物并将所述微生物施用于植物的环境。