Method of Proteome Analysis for Phosphorylated Protein
    11.
    发明申请
    Method of Proteome Analysis for Phosphorylated Protein 失效
    磷酸化蛋白质的蛋白质组分析方法

    公开(公告)号:US20080221802A1

    公开(公告)日:2008-09-11

    申请号:US11630927

    申请日:2005-07-04

    IPC分类号: G01N33/68 C07K1/14

    摘要: A method for detecting plural types of phosphorylated proteins in a sample, wherein a database consisting of data regarding plural types of proteins in the sample is used; and a method for purifying phosphorylated proteins using an immobilized metal carrier or a titania carrier, wherein a solution containing acetonitrile in a range of 40% (v/v) or greater but 60% (v/v) or less is used.

    摘要翻译: 一种用于检测样品中多种磷酸化蛋白质的方法,其中使用由样本中关于多种蛋白质的数据组成的数据库; 以及使用固定金属载体或二氧化钛载体来纯化磷酸化蛋白质的方法,其中使用含有40%(v / v)以上且60%(v / v)以下范围内的乙腈的溶液。

    Method of Analyzing Protein Structure Affinity Relationship
    12.
    发明申请
    Method of Analyzing Protein Structure Affinity Relationship 失效
    分析蛋白质结构亲和关系的方法

    公开(公告)号:US20080057592A1

    公开(公告)日:2008-03-06

    申请号:US11571337

    申请日:2005-07-04

    申请人: Yoshiya Oda

    发明人: Yoshiya Oda

    IPC分类号: G01N33/00

    摘要: A method for analyzing a structural affinity relationship between plural kinds of proteins and a compound, comprising the steps of (a) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of isotope-labeled proteins; (b) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of proteins brought into contact with a compound beforehand; (c) mixing the proteins obtained in step (a) and step (b); (d) analyzing the mixture obtained in step (c) with mass spectrometry; (e) identifying each of plural kinds of proteins based on information obtained by the mass spectrometry; and (f) obtaining an intensity ratio between a labeled peak and a non-labeled peak of each protein, thereby quantitating an affinity ratio of the compound to each protein.

    摘要翻译: 一种用于分析多种蛋白质和化合物之间的结构亲和关系的方法,包括以下步骤:(a)使用固定化合物的载体从载体上与所述化合物结合的多种蛋白质从一组​​同位素 - 标记蛋白; (b)使用化合物固定化载体从预先与化合物接触的一组蛋白质中纯化与载体上的化合物结合的多种蛋白质; (c)混合步骤(a)和步骤(b)中获得的蛋白质; (d)用质谱分析步骤(c)中得到的混合物; (e)基于通过质谱法获得的信息来鉴定多种蛋白质中的每一种; 和(f)获得每种蛋白质的标记峰和非标记峰之间的强度比,从而定量化合物与每种蛋白质的亲和比。