公开(公告)号：US20190161726A1

公开(公告)日：2019-05-30

申请号：US16321265

申请日：2017-07-28

Applicant: DAIKIN INDUSTRIES, LTD. ,  KYOTO UNIVERSITY

Inventor： Kozue KOMAZAWA ,  Tatsuya HIGUCHI ,  Shin KANEKO ,  Yutaka YASUI

IPC: C12N5/0735 ,  C12M1/00 ,  C09D127/18 ,  C08F14/26 ,  C08F214/26 ,  C08F214/28 ,  C12N5/074

Abstract: A method for producing differentiated cells from pluripotent stem cells by an embryoid body (EB) method, including the steps of: (1) culturing pluripotent stem cells by using a culture bag having a perfluoropolymer on its inner surface to thereby form an embryoid body; and (2) inducing differentiation of the pluripotent stem cells contained in the embryoid body obtained in step (1) to thereby obtain differentiated cells.

公开(公告)号：US20230210904A1

公开(公告)日：2023-07-06

申请号：US18145476

申请日：2022-12-22

Applicant: KYOTO UNIVERSITY

Inventor： Shin KANEKO ,  Norihiro UEDA ,  Yasushi UEMURA ,  Kyoko FUKUDA

IPC: A61K35/17 ,  C12N5/0789 ,  C12N15/86 ,  C12N15/90

CPC classification number: A61K35/17 ,  C12N5/0647 ,  C12N15/86 ,  C12N15/90 ,  C12N2501/125 ,  C12N2501/165 ,  C12N2501/2302 ,  C12N2501/2315 ,  C12N2501/26 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2502/1394

Abstract: A pharmaceutical includes helper T cells induced from pluripotent stem cells. The helper T cells include CD4-positive CD40L-highly expressing T cells, dendritic cells, antigen, and cytotoxic T cells CD8-positive T cells. The pharmaceutical can be administered in a method for treating cancer to a patient having cancer cells expressing an antigen specifically recognized by CD4-positive T cells.

公开(公告)号：US20220062341A1

公开(公告)日：2022-03-03

申请号：US17418457

申请日：2019-12-26

Applicant: KIRIN HOLDINGS KABUSHIKI KAISHA ,  KYOTO UNIVERSITY

Inventor： Shinichiro TAKAYANAGI ,  Saki HASEGAWA ,  Ken FUKUMOTO ,  Atsushi KUNISATO ,  Satoshi NISHIKAWA ,  Shin KANEKO

IPC: A61K35/17 ,  C07K14/725 ,  C12N15/63

Abstract: An object of the invention is to provide a modified T-cell receptor (TCR) which does not cause the antigen responsiveness and which is capable of holding CD3 subunits on a cell membrane and causing the CD3 subunits to function. The invention is an invention related to a modified TCR comprising a first polypeptide and a second polypeptide, wherein the first polypeptide is a polypeptide which comprises a constant region of human TCRα or a fragment of the constant region and which does not comprise a variable region of human TCRα, and the second polypeptide is a polypeptide which comprises a constant region of human TCRβ or a fragment of the constant region and which does not comprise a variable region of human TCRβ.

公开(公告)号：US20190330596A1

公开(公告)日：2019-10-31

申请号：US16312789

申请日：2017-06-21

Applicant: KYOTO UNIVERSITY

Inventor： Shin KANEKO ,  Yutaka YASUI ,  Shoichi IRIGUCHI ,  Tatsuki UEDA

IPC: C12N5/074 ,  C12N5/10 ,  C12N15/85 ,  C12N5/0783 ,  C07K14/725 ,  C07D401/04

Abstract: A method for producing CD4/CD8 double-positive T cells, comprising the steps of: (1) culturing pluripotent stem cells in a medium to induce hematopoietic progenitor cells; and (2) culturing the hematopoietic progenitor cells obtained in the step (1) in a medium containing a p38 inhibitor and/or SDF-1 to induce CD4/CD8 double-positive T cells.

公开(公告)号：US20180298337A1

公开(公告)日：2018-10-18

申请号：US15767917

申请日：2016-10-14

Applicant: Kyoto University

Inventor： Shin KANEKO ,  Norihiro UEDA ,  Yasushi UEMURA

IPC: C12N5/0783 ,  C12N15/86 ,  C12N5/0735 ,  A61K35/17

Abstract: An object of the present invention is induction of CD4-positive T cells from pluripotent stem cells. This object is achieved by production of CD4-positive T cells by introducing a CD4 gene or a gene product thereof into T cells induced from pluripotent stem cells.