公开(公告)号：US20240360477A1

公开(公告)日：2024-10-31

申请号：US18598724

申请日：2024-03-07

申请人： Metagenomi, Inc.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Daniela S.A. GOLTSMAN ,  Sarah LAPERRIERE ,  Cindy CASTELLE ,  Lisa ALEXANDER ,  Mary Kaitlyn Chiu ,  Morayma TEMOCHE-DIAZ ,  Anu THOMAS

IPC分类号： C12N15/90 ,  C12N9/12

CPC分类号： C12N15/907 ,  C12N9/1276 ,  C12N15/90 ,  C12Y207/07049 ,  C12N2800/90

摘要： The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods may comprise a first double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a retrotransposase, and the retrotransposase, wherein said retrotransposase is configured to transpose the cargo nucleotide sequence to the target nucleic acid site.

公开(公告)号：US20240271162A1

公开(公告)日：2024-08-15

申请号：US18568638

申请日：2022-06-10

申请人： LifeEDIT Therapeutics, Inc.

发明人： Tyson D. Bowen ,  Alexandra Briner Crawley ,  Tedd D. Elich

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11

CPC分类号： C12N15/90 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11 ,  C12Y305/04 ,  C12N2310/20

摘要： Compositions and methods useful for expressing an RNA are provided. Compositions comprise nucleic acid molecules comprising an RNA polymerase III promoter and expression constructs comprising the novel promoters operably linked to a coding sequence. The promoters can initiate transcription of RNA-coding nucleotide sequences through the recruitment of RNA polymerase III (pol III) or, in some embodiments, polypeptide-encoding nucleotide sequences through the recruitment of RNA polymerase II. The promoters thus find use in transcribing nucleotide sequences, including but not limited to, those encoding guide RNAs, and polypeptide-encoding sequences. Such expression constructs comprising the novel promoters operably linked to nucleotide sequences encoding guide RNAs find use in binding and/or modifying the sequence of a target nucleic acid molecule, modifying the expression of a target gene, or detecting a target nucleotide sequence in cells comprising an RNA-guided nuclease (RGN) or RNA-guided nucleotide-binding polypeptide (RGNBP).

公开(公告)号：US20240263130A1

公开(公告)日：2024-08-08

申请号：US18031556

申请日：2022-06-28

申请人： SHENZHEN BLUEPHA BIOSCIENCES CO., LTD.

发明人： Jin YIN ,  Yu WANG ,  Jiajia LI ,  Jie HOU ,  Liang ZOU ,  Zixian CHEN

IPC分类号： C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/74 ,  C12N15/90 ,  C12P7/625 ,  C12R1/01

CPC分类号： C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/74 ,  C12N15/90 ,  C12P7/625 ,  C12N2510/00 ,  C12R2001/01 ,  C12Y101/01036 ,  C12Y402/01119

摘要： The present invention relates to the technical field of microorganisms, and specifically to engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxyhexanoic acid in PHA. The acetoacetyl-CoA reductase variants and their coding genes provided by the present invention can significantly increase the proportion of 3-hydroxyhexanoic acid in PHA produced by strains; the proportion of 3-hydroxyhexanoic acid in PHA produced by the engineered Ralstonia eutropha constructed utilizing the acetoacetyl-CoA reductase variants and their coding genes provided by the present invention is significantly increased, which provides new genes and strain resources for strains producing poly(3-hydroxybutyrate-co-3-10 the development of engineered hydroxyhexanoate) with high proportion of 3-hydroxyhexanoic acid.

公开(公告)号：US12054757B1

公开(公告)日：2024-08-06

申请号：US18424759

申请日：2024-01-27

申请人： Hubei University

发明人： Lixin Ma ,  Longyu Wang ,  Fei Wang ,  Xiaochen Xie ,  Wanping Chen ,  Fuyong Huang

IPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12Q1/6823

CPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12Q1/6823 ,  C12Y301/00 ,  C12N2310/20 ,  C12N2800/80

摘要： A Pyrococcus furiosus Argonaute (PfAgo) mutant protein with mesophilic target nucleic acid cleavage activity and an application thereof are provided. The PfAgo mutant protein has an amino acid mutation on at least one of a 617th position and a 618th position relative to the sequence shown in SEQ ID NO: 1, specifically, K617E/G and/or L618Y/F/W/G. Compared with a wild-type PfAgo protein, the provided PfAgo mutant protein has activity in the temperature range of 30-95 Celsius degree (° C.), effectively expanding application range of prokaryotic Argonaute (pAgo) protein.

公开(公告)号：US12054702B2

公开(公告)日：2024-08-06

申请号：US17150878

申请日：2021-01-15

申请人： DNA TWOPOINTO INC.

发明人： Jeremy Minshull ,  Mark Welch ,  Sridhar Govindrajan ,  Maggie Lee ,  Kate Caves ,  Jon Ness

IPC分类号： C12N9/00 ,  A61K8/66 ,  A61K47/42 ,  C07K14/435 ,  C12N1/16 ,  C12N1/18 ,  C12N9/12 ,  C12N15/10 ,  C12N15/52 ,  C12N15/62 ,  C12N15/63 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C12N15/67 ,  C12R1/84 ,  C12R1/865

CPC分类号： C12N1/165 ,  C12N1/185 ,  C12N9/12 ,  C12N9/1241 ,  C12N15/1082 ,  C12N15/52 ,  C12N15/625 ,  C12N15/63 ,  C12N15/81 ,  C12N15/815 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/90 ,  C12Y207/00 ,  C12Y207/07 ,  C07K2319/09 ,  C12N15/67 ,  C12N2800/90 ,  C12N2830/007 ,  C12N2830/40 ,  C12N2830/42 ,  C12N2840/203 ,  C12R2001/84 ,  C12R2001/865

摘要： The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

公开(公告)号：US20240252550A1

公开(公告)日：2024-08-01

申请号：US18486067

申请日：2024-04-05

申请人： Beam Therapeutics Inc.

发明人： Giuseppe CIARAMELLA ,  Jason Michael GEHRKE ,  Ryan MURRAY

IPC分类号： A61K35/407 ,  A61P1/16 ,  C12N5/071 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11 ,  C12N15/90

CPC分类号： A61K35/407 ,  A61P1/16 ,  C12N5/067 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11 ,  C12N15/90 ,  C12Y305/04 ,  C12N2310/20 ,  C12N2510/00

摘要： The present invention provides methods of producing genetically modified human hepatocytes suitable for hepatocyte transplantation comprising: disrupting one or more major histocompatibility complex (MHC) Class I or Class II genes in isolated human hepatocytes or in a hepatocyte progenitor cell by introducing a base editor and one or more gRNAs that hybridize with a target sequence in the one or more Class I or Class II genes, thereby producing genetically modified human hepatocytes.

公开(公告)号：US20240229011A1

公开(公告)日：2024-07-11

申请号：US18543827

申请日：2023-12-18

申请人： OHIO STATE INNOVATION FOUNDATION

发明人： Yizhou DONG ,  Bin LI

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/113 ,  C12N15/90

CPC分类号： C12N15/102 ,  C12N9/22 ,  C12N15/113 ,  C12N15/90 ,  C12N2310/113 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3521 ,  C12N2310/3533

摘要： The present disclosure generally relates to genome editing systems and methods and compounds and compositions for use in Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) genome editing systems. Disclosed herein are modified nucleic acids that modulate the activity of genome editing.

公开(公告)号：US12029760B2

公开(公告)日：2024-07-09

申请号：US16609628

申请日：2018-05-02

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： Yangbing Zhao ,  Jiangtao Ren

IPC分类号： A61K35/17 ,  C12N9/22 ,  C12N15/10 ,  C12N15/117 ,  C12N15/90 ,  A61K39/00 ,  A61P35/00 ,  A61P37/02

CPC分类号： A61K35/17 ,  C12N9/22 ,  C12N15/102 ,  C12N15/117 ,  C12N15/90 ,  A61K2039/5158 ,  A61P35/00 ,  A61P37/02 ,  C12N2310/122 ,  C12N2310/20 ,  C12N2310/315

摘要： The present invention includes compositions and methods for modifying primary T cells. In one aspect, the invention comprises administering to a cell a stem-loop Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) RNA (st-crRNA) and a Cpf1 enzyme.

公开(公告)号：US11987804B2

公开(公告)日：2024-05-21

申请号：US17049782

申请日：2019-04-25

申请人： Seattle Children's Hospital

发明人： Andrew M. Scharenberg ,  David J. Rawlings ,  Karen Sommer ,  Samuel West ,  Yuchi Chiang Honaker ,  Ryo Takeuchi

IPC分类号： C12N15/90 ,  C07K14/715 ,  C12N15/113 ,  C12N15/86

CPC分类号： C12N15/90 ,  C07K14/7155 ,  C12N15/113 ,  C12N15/86 ,  C07K2317/53 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2740/16043 ,  C12N2750/14143

摘要： The present application relates to, inter alia, compositions including proteins for expression in host cells to render them resistant to rapamycin. The application further relates to methods of using the proteins, cells, and compositions disclosed therein for modulating cell signaling and for selective expansion of cells.

公开(公告)号：US11970711B2

公开(公告)日：2024-04-30

申请号：US17481063

申请日：2021-09-21

申请人： The Regents of the University of California ,  University of Vienna ,  Emmanuelle Charpentier

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/11 ,  A01H6/46 ,  A01K67/027 ,  A61K38/46 ,  C12N9/22 ,  C12N15/10 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/90 ,  C12Q1/686 ,  H01L21/02 ,  H01L33/00 ,  A61K48/00

CPC分类号： C12N15/907 ,  A01H6/4684 ,  A01K67/027 ,  A61K38/465 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/746 ,  C12N15/90 ,  C12N15/902 ,  C12Q1/686 ,  H01L21/02312 ,  H01L21/02365 ,  H01L33/0075 ,  A61K48/00 ,  C12N2310/11 ,  C12N2310/13 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/31 ,  C12N2310/32 ,  C12N2310/33 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/80 ,  C12Y301/04

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.