公开(公告)号：US20090286304A1

公开(公告)日：2009-11-19

申请号：US12463780

申请日：2009-05-11

申请人： Gary Latham ,  Brittan L. Pasloske ,  Heidi Peltier

发明人： Gary Latham ,  Brittan L. Pasloske ,  Heidi Peltier

IPC分类号： C12N1/08

CPC分类号： C12Q1/6806 ,  C12N15/1003

摘要： The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.

摘要翻译： 本发明涉及从含细胞的样品中分离核酸的组合物和方法。 公开了一种方法，包括获得至少一种含细胞的样品，其包含含有核酸的细胞，获得至少一种分解代谢酶，获得至少一种核酸酶抑制剂，制备样品，分解代谢酶和 核酸酶抑制剂，在分解代谢酶活性的条件下保持混合物，并搅拌混合物，其中样品被消化以产生样品的含核酸裂解物。

公开(公告)号：US20060228731A1

公开(公告)日：2006-10-12

申请号：US11361739

申请日：2006-02-23

申请人： Gary Latham ,  Jon Kemppainen

发明人： Gary Latham ,  Jon Kemppainen

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C12N9/22 ,  C12N1/18 ,  C12N15/74

CPC分类号： C12N9/22

摘要： Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5′-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.

摘要翻译： 公开了制备和使用合成牛DNase I的组合物和方法。 更具体地，本发明的sbDNase I是非特异性切割DNA以释放5'-磷酸化核苷酸的通用酶。 本发明的sbDNase I分子在广泛的分子生物学应用中特别有用，包​​括：RNA分离后污染DNA的降解; 在体外转录之前或之后与RT-PCR结合进行RNA清除; 鉴定DNA上的蛋白质结合序列（DNase I footprinting）; 处理培养细胞时防止结块; 组织解离和产生用于体外重组反应的片段DNA。

公开(公告)号：US07067298B2

公开(公告)日：2006-06-27

申请号：US10420345

申请日：2003-04-22

申请人： Gary Latham ,  Jon Kemppainen

发明人： Gary Latham ,  Jon Kemppainen

IPC分类号： C12N9/22 ,  C12N15/55

CPC分类号： C12N9/22

摘要： Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5′-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.

摘要翻译： 公开了制备和使用合成牛DNase I的组合物和方法。 更具体地，本发明的sbDNase I是非特异性切割DNA以释放5'-磷酸化核苷酸的通用酶。 本发明的sbDNase I分子在广泛的分子生物学应用中特别有用，包​​括：RNA分离后污染DNA的降解; 在体外转录之前或之后与RT-PCR结合进行RNA清除; 鉴定DNA上的蛋白质结合序列（DNase I footprinting）; 处理培养细胞时防止结块; 组织解离和产生用于体外重组反应的片段DNA。