公开(公告)号：US20090286304A1

公开(公告)日：2009-11-19

申请号：US12463780

申请日：2009-05-11

申请人： Gary Latham ,  Brittan L. Pasloske ,  Heidi Peltier

发明人： Gary Latham ,  Brittan L. Pasloske ,  Heidi Peltier

IPC分类号： C12N1/08

CPC分类号： C12Q1/6806 ,  C12N15/1003

摘要： The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.

摘要翻译： 本发明涉及从含细胞的样品中分离核酸的组合物和方法。 公开了一种方法，包括获得至少一种含细胞的样品，其包含含有核酸的细胞，获得至少一种分解代谢酶，获得至少一种核酸酶抑制剂，制备样品，分解代谢酶和 核酸酶抑制剂，在分解代谢酶活性的条件下保持混合物，并搅拌混合物，其中样品被消化以产生样品的含核酸裂解物。

公开(公告)号：US20060188892A1

公开(公告)日：2006-08-24

申请号：US11076455

申请日：2005-03-09

申请人： Gary Latham ,  Brittan Pasloske ,  Heidi Peltier

发明人： Gary Latham ,  Brittan Pasloske ,  Heidi Peltier

IPC分类号： C12Q1/68 ,  C12N1/08

CPC分类号： C12Q1/6806 ,  C12N15/1003

摘要： The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.

摘要翻译： 本发明涉及从含细胞的样品中分离核酸的组合物和方法。 公开了一种方法，包括获得至少一种含细胞的样品，其包含含有核酸的细胞，获得至少一种分解代谢酶，获得至少一种核酸酶抑制剂，制备样品，分解代谢酶和 核酸酶抑制剂，在分解代谢酶活性的条件下保持混合物，并搅拌混合物，其中样品被消化以产生样品的含核酸裂解物。

公开(公告)号：US20050232934A1

公开(公告)日：2005-10-20

申请号：US10827498

申请日：2004-04-19

申请人： Liangjing Chen ,  Robert Setterquist ,  Gary Latham

发明人： Liangjing Chen ,  Robert Setterquist ,  Gary Latham

IPC分类号： A61K39/21 ,  C07H21/04 ,  C12N9/12 ,  C12N9/22 ,  C12N15/867

CPC分类号： C12N9/1276 ,  C12Y207/07049

摘要： The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

摘要翻译： 本发明涉及编码具有DNA聚合酶活性和显着降低的RNA酶H活性的多反应逆转录酶的基因，含有本发明转化的基因和宿主细胞的载体。 本发明还包括使用本发明的逆转录酶产生过度逆转录酶，从mRNA产生cDNA的方法，使用过度反转录酶制备的试剂盒和测定模板。

公开(公告)号：US20070032418A1

公开(公告)日：2007-02-08

申请号：US10546855

申请日：2004-02-25

申请人： Robert Shapiro ,  Jeremy Jenkins ,  Richard Kao ,  Gary Latham

发明人： Robert Shapiro ,  Jeremy Jenkins ,  Richard Kao ,  Gary Latham

IPC分类号： A61K38/22 ,  A61K31/655 ,  A61K31/555 ,  A61K31/409 ,  C07D487/22

CPC分类号： A61K31/015 ,  A61K31/15 ,  A61K31/167 ,  A61K38/39 ,  A61K45/06 ,  Y02A50/473 ,  A61K2300/00

摘要： Lead compounds were obtained in a high throughput screen (HTS) of angiogenin (ANG; a potent inducer of angiogenesis) enzyme activity, an RNase. One lead was shown to delay appearance of tumors in an animal tumor system, and to reduce the number of animals having tumors. Several lead compound analogs were found to be even more potent inhibitors of ANG activity compared to the original leads, and two were also found to have greater affinity for ANG than for pancreatic RNase. Other embodiments disclose a method comprising obtaining a ribonuclease inhibitor and a composition; and admixing the ribonuclease inhibitor and the composition to form an admixture, wherein a ribonuclease that may be present in the admixture is inhibited.

摘要翻译： 在血管生成素（ANG，血管发生的有效诱导剂）酶活性的高通量筛选（HTS），RNA酶中获得铅化合物。 显示出一个导联可以延缓肿瘤在动物肿瘤系统中的出现，并减少具有肿瘤的动物的数量。 与原始引物相比，发现几种铅化合物类似物是更有效的ANG活性抑制剂，并且还发现两种与ANG相比对于胰腺RNase具有更大的亲和力。 其他实施方案公开了包括获得核糖核酸酶抑制剂和组合物的方法; 并混合核糖核酸酶抑制剂和组合物以形成混合物，其中可以存在于混合物中的核糖核酸酶被抑制。

公开(公告)号：US20050175268A1

公开(公告)日：2005-08-11

申请号：US11048181

申请日：2005-02-01

申请人： Gary Latham

发明人： Gary Latham

IPC分类号： F16C33/10 ,  F16C33/66 ,  F16N3/10 ,  F16N5/02 ,  F16C19/00

CPC分类号： F16C33/6622 ,  F16C19/06 ,  F16C35/042 ,  F16N2210/14

摘要： A lubricating device and method for delivering fresh grease to a rolling element bearing in a first cavity, by by-passing all existing grease in the first cavity and in other cavities surrounding and/or leading to the rolling element bearing, and delivering fresh grease in close proximity to the rolling element bearing. A tube is disposed between the zerk fitting and the bearing to ensure delivery of the fresh new grease to the bearing.

摘要翻译： 一种润滑装置和方法，用于将新鲜的油脂递送到第一腔中的滚动元件轴承，通过绕过第一腔中的所有存在的润滑脂以及围绕和/或通向滚动元件轴承的其它空腔，并将新鲜的油脂输送到 靠近滚动体轴承。 一个管子设置在夹头配件和轴承之间，以确保将新鲜的新润滑脂输送到轴承上。

公开(公告)号：US20050014169A1

公开(公告)日：2005-01-20

申请号：US10786875

申请日：2004-02-25

申请人： Gary Latham ,  Matthew Winkler ,  Brittan Pasloske ,  W. Kudlicki

发明人： Gary Latham ,  Matthew Winkler ,  Brittan Pasloske ,  W. Kudlicki

IPC分类号： C07K16/40 ,  C12N15/10 ,  C12Q1/34 ,  G01N33/573 ,  C12Q1/68 ,  C12N9/99

CPC分类号： C12N15/1003 ,  C07K16/40 ,  C12N15/1096 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

摘要： Methods and compositions for inhibiting and/or inactivating nucleases by using nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors.

摘要翻译： 提供了通过使用核酸酶抑制剂抑制和/或灭活核酸酶的方法和组合物。 核酸酶抑制剂包括抗核酸酶抗体和非抗体核酸酶抑制剂。

公开(公告)号：US20140024035A1

公开(公告)日：2014-01-23

申请号：US13946544

申请日：2013-07-19

申请人： Ashish Choudhary ,  Gary Latham ,  Sachin Sah

发明人： Ashish Choudhary ,  Gary Latham ,  Sachin Sah

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  G06F19/18

摘要： Disclosed herein are methods for the automated reconstruction of a genotype of a gene, fragment, or genomic region using exhaustive enumeration. The methods can be used to reconstruct the genotype of any GC-rich sequence, such as the CGG repeat region in the 5′ UTR of FMR1 or the CCG repeat region in the 5′ UTR of FMR2. Also disclosed is an apparatus for use in conducting automated genotype reconstruction, as well as methods of diagnosis and treatment using exhaustive enumeration methods to reconstruct and identify genotypes associated with a disease or disorder.

摘要翻译： 本文公开了使用详尽枚举来自动重建基因，片段或基因组区域的基因型的方法。 该方法可用于重建任何富含GC的序列的基因型，如FMR1的5'UTR中的CGG重复区或FMR2的5'UTR中的CCG重复区。 还公开了一种用于进行自动基因型重建的装置，以及使用穷举枚举方法来重建和鉴定与疾病或病症相关的基因型的诊断和治疗方法。

公开(公告)号：US08361754B2

公开(公告)日：2013-01-29

申请号：US12568364

申请日：2009-09-28

申请人： Liangjing Chen ,  Robert A. Setterquist ,  Gary Latham

发明人： Liangjing Chen ,  Robert A. Setterquist ,  Gary Latham

IPC分类号： C12P19/34

CPC分类号： C12N9/1276 ,  C12Y207/07049

摘要： The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

公开(公告)号：US20110318811A1

公开(公告)日：2011-12-29

申请号：US13103798

申请日：2011-05-09

申请人： Gary Latham ,  Jon Kemppainen

发明人： Gary Latham ,  Jon Kemppainen

IPC分类号： C12N9/22

CPC分类号： C12N9/22

摘要： Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5′-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.

摘要翻译： 公开了制备和使用合成牛DNase I的组合物和方法。 更具体地，本发明的sbDNase I是非特异性切割DNA以释放5'-磷酸化核苷酸的多功能酶。 本发明的sbDNase I分子在广泛的分子生物学应用中特别有用，包​​括：RNA分离后污染DNA的降解; 在体外转录之前或之后与RT-PCR结合进行RNA清除; 鉴定DNA上的蛋白质结合序列（DNase I footprinting）; 处理培养细胞时防止结块; 组织解离和产生用于体外重组反应的片段DNA。

公开(公告)号：US20100203597A1

公开(公告)日：2010-08-12

申请号：US12568364

申请日：2009-09-28

申请人： Liangjing Chen ,  Robert A. Setterquist ,  Gary Latham

发明人： Liangjing Chen ,  Robert A. Setterquist ,  Gary Latham

IPC分类号： C12P19/34 ,  C12N9/12 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C07H21/02

CPC分类号： C12N9/1276 ,  C12Y207/07049

摘要： The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

摘要翻译： 本发明涉及编码具有DNA聚合酶活性和显着降低的RNA酶H活性的多反应逆转录酶的基因，含有本发明转化的基因和宿主细胞的载体。 本发明还包括使用本发明的逆转录酶产生过度逆转录酶，从mRNA产生cDNA的方法，使用过度反转录酶制备的试剂盒和测定模板。