RECOMBINANT DNA CONSTRUCTS EMPLOYING SITE-SPECIFIC RECOMBINATION
    18.
    发明申请
    RECOMBINANT DNA CONSTRUCTS EMPLOYING SITE-SPECIFIC RECOMBINATION 有权
    使用现场特异性重组的重组DNA构建体

    公开(公告)号:US20120054884A1

    公开(公告)日:2012-03-01

    申请号:US12869806

    申请日:2010-08-27

    摘要: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.

    摘要翻译: 用于植物和植物细胞的重组DNA构建体具有位点特异性重组位点,其允许通过过表达或抑制内源基因来评估表型和作用模式。 在一方面,单个DNA构建体可以通过重组酶(例如Cre重组酶)对具有lox重组位点的构建体的作用在过度表达和抑制之间切换。 其他有用的重组系统包括Flp / frt系统,R / Rs系统,Dre / rox系统和GIN / gix系统。

    Recombinant DNA constructs employing site-specific recombination
    19.
    发明授权
    Recombinant DNA constructs employing site-specific recombination 有权
    使用位点特异性重组的重组DNA构建体

    公开(公告)号:US08816153B2

    公开(公告)日:2014-08-26

    申请号:US12869806

    申请日:2010-08-27

    IPC分类号: C12N15/11 C12N15/82 C12N5/10

    摘要: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.

    摘要翻译: 用于植物和植物细胞的重组DNA构建体具有位点特异性重组位点,其允许通过过表达或抑制内源基因来评估表型和作用模式。 在一方面,单个DNA构建体可以通过重组酶(例如Cre重组酶)对具有lox重组位点的构建体的作用在过度表达和抑制之间切换。 其他有用的重组系统包括Flp / frt系统,R / Rs系统,Dre / rox系统和GIN / gix系统。