公开(公告)号：US20080124762A1

公开(公告)日：2008-05-29

申请号：US11831882

申请日：2007-07-31

申请人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

发明人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

IPC分类号： C12P21/04 ,  C12N15/00 ,  C12N5/06

CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

摘要： Recombinant expression vectors are provided comprising a 3′ UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。

公开(公告)号：US4770832A

公开(公告)日：1988-09-13

申请号：US758721

申请日：1985-07-25

申请人： Tadashi Okamoto ,  Sumiyuki Matsubara ,  Koichi Hasuo ,  Masahisa Handa

发明人： Tadashi Okamoto ,  Sumiyuki Matsubara ,  Koichi Hasuo ,  Masahisa Handa

IPC分类号： B29C35/02 ,  B29C53/56 ,  B29C70/20 ,  B29C70/50 ,  B29C70/54 ,  B29D99/00 ,  E04C5/07 ,  B29C59/02

CPC分类号： B29D99/0003 ,  B29C53/56 ,  B29C70/20 ,  B29C70/50 ,  B29C70/542 ,  E04C5/07 ,  B29C35/02 ,  B29L2031/06

摘要： A process relating to the manufacturing of a structural reinforcing material which is made of a glass fiber reinforced plastic, a carbon fiber reinforced plastic or similar material wherein a precise sectional shape is not required. A fiber body including a plurality of reinforcing fibers is impregnated with a hardenable material and subsequently introduced into a drawing die having a desired sectional shape so as to be formed into a drawn article having a predetermined sectional shape. A tape is coiled therearound so as to cover the article. A string member is coiled therearound to thrust into the surface of the article. Thereafter, the drawn fiber body is subjected to a hardening treatment and the tape and string member are removed.

摘要翻译： 涉及制造由玻璃纤维增​​强塑料，碳纤维增强塑料或类似材料制成的结构增强材料的方法，其中不需要精确的截面形状。 包含多根增强纤维的纤维体浸渍有可硬化材料，随后将其引入具有所需截面形状的拉伸模具中，以形成具有预定截面形状的拉伸制品。 磁带绕在其周围，以覆盖物品。 弦构件卷绕在其周围以推入制品的表面。 此后，对拉伸的纤维体进行硬化处理，并除去带和绳构件。