-
公开(公告)号：US5795589A
公开(公告)日：1998-08-18
申请号：US794988
申请日：1997-02-05
申请人： Lawrence D. Mayer , Marcel B. Bally , Pieter R. Cullis , Richard S. Ginsberg , George N. Mitilenes
发明人： Lawrence D. Mayer , Marcel B. Bally , Pieter R. Cullis , Richard S. Ginsberg , George N. Mitilenes
CPC分类号： A61K9/1278 , A61K9/127 , Y10S436/826 , Y10S436/829 , Y10S514/908 , Y10T428/2984
摘要： A method for encapsulation of antineoplastic agents in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes may be made by a process that loads the drug by an active mechanism using a transmembrane ion gradient, preferably a tranamembrane pH gradient. Using this technique, trapping efficiencies approach 100%, and liposomes may be loaded with drug immediately prior to use, eliminating stability problem related to drug retention in the liposomes. Drug:lipid ratios employed are about 3-80 fold higher than for traditional liposome preparations, and the release rate of the drug from the liposomes is reduced. An assay method to determine free antineoplastic agents in a liposome preparation is also disclosed.
摘要翻译： 提供了在脂质体中包封抗肿瘤剂的方法，优选具有高的药物：脂质比。 脂质体可以通过使用跨膜离子梯度，优选跨膜pH梯度的活性机制来加载药物的方法制备。 使用这种技术，捕获效率接近100％，脂质体可以在使用前立即加载药物，消除与脂质体中药物滞留有关的稳定性问题。 药物：使用的脂质比例比传统脂质体制剂高约3-80倍，并且药物从脂质体的释放速率降低。 还公开了一种在脂质体制剂中测定游离抗肿瘤剂的测定方法。
-
公开(公告)号：US5616341A
公开(公告)日：1997-04-01
申请号：US112875
申请日：1993-08-26
申请人： Lawrence D. Mayer , Marcel B. Bally , Pieter R. Cullis , Richard S. Ginsberg , George N. Mitilenes
发明人： Lawrence D. Mayer , Marcel B. Bally , Pieter R. Cullis , Richard S. Ginsberg , George N. Mitilenes
CPC分类号： A61K9/1278 , A61K9/127 , Y10S436/826 , Y10S436/829 , Y10S514/908 , Y10T428/2984
摘要： A method for encapsulation of antineoplastic agents in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes may be made by a process that loads the drug by an active mechanism using a transmembrane ion gradient, preferably a transmembrane pH gradient. Using this technique, trapping efficiencies approach 100%, and liposomes may be loaded with drug immediately prior to use, eliminating stability problems related to drug retention in the liposomes. Drug:lipid ratios employed are about 3-80 fold higher than for traditional liposome preparations, and the release rate of the drug from the liposomes is reduced. An assay method to determine free antineoplastic agents in a liposome preparation is also disclosed.
摘要翻译： 提供了在脂质体中包封抗肿瘤剂的方法，优选具有高的药物：脂质比。 脂质体可以通过使用跨膜离子梯度，优选跨膜pH梯度的活性机制加载药物的方法制备。 使用这种技术，捕获效率接近100％，脂质体可以在使用前立即加载药物，消除与脂质体中药物滞留有关的稳定性问题。 药物：使用的脂质比例比传统脂质体制剂高约3-80倍，并且药物从脂质体的释放速率降低。 还公开了一种在脂质体制剂中测定游离抗肿瘤剂的测定方法。
-
公开(公告)号：US5077056A
公开(公告)日：1991-12-31
申请号：US284751
申请日：1988-12-12
IPC分类号： A61K9/127
CPC分类号： A61K9/1278 , A61K9/1277 , Y10S436/829
摘要： Methods for encapsulating ionizable antineoplastic agents in liposomes using transmembrane potentials are provided. Trapping efficiencies approaching 100% and rapid loading are readily achieved. Dehydration protocols which allow liposomes to be conveniently used in the administration of antineoplastic agents in a clinical setting are also provided. In accordance with other aspects of the invention, transmembrane potentials are used to reduce the rate of release of ionizable drugs from liposomes.
摘要翻译： 提供了使用跨膜电位将可电离抗肿瘤剂包封在脂质体中的方法。 捕获效率接近100％，快速加载容易实现。 还提供允许脂质体在临床环境中方便地用于施用抗肿瘤剂的脱水方案。 根据本发明的其他方面，使用跨膜电位来降低可离子化药物从脂质体释放的速率。
-
14.发明授权Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer 失效通过疏水性脂质 - 核酸复合物中间体制备并用于基因转移的脂质 - 核酸颗粒公开(公告)号：US07422902B1
公开(公告)日：2008-09-09
申请号：US09431594
申请日：1999-11-01
申请人： Jeffery J. Wheeler , Marcel B. Bally , Yuan-Peng Zhang , Dorothy L. Reimer , Michael Hope , Pieter R. Cullis , Peter Scherrer
发明人： Jeffery J. Wheeler , Marcel B. Bally , Yuan-Peng Zhang , Dorothy L. Reimer , Michael Hope , Pieter R. Cullis , Peter Scherrer
CPC分类号： A61K9/1272 , A61K47/6911 , C12N15/88 , Y10S436/829
摘要： Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or an organic solvent) the lipid-nucleic acid complexes form particles wherein the nucleic acid is serum-stable and is protected from degradation. The particles thus formed have access to extravascular sites and target cell populations and are suitable for the therapeutic delivery of nucleic acids.
摘要翻译： 描述了可用于体外或体内基因转移的新型脂质 - 核酸颗粒复合物。 可以使用洗涤剂透析方法或利用有机溶剂的方法形成颗粒。 在除去增溶组分（即洗涤剂或有机溶剂）后，脂质 - 核酸复合物形成其中核酸是血清稳定的并且被保护免于降解的颗粒。 由此形成的颗粒可以进入血管外部位和靶细胞群，并且适合于治疗性递送核酸。
-
公开(公告)号：US06534484B1
公开(公告)日：2003-03-18
申请号：US09436933
申请日：1999-11-08
IPC分类号： A01N4304
CPC分类号： C12N15/88 , A61K9/1272 , Y10S436/829 , Y10S514/851 , Y10S977/80 , Y10S977/907
摘要： Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stable.
摘要翻译： 描述了可用于体外或体内转染细胞的质粒 - 脂质颗粒。 可以使用洗涤剂透析方法或利用有机溶剂的方法形成颗粒。 颗粒通常为65-85nm，完全包封质粒并且是血清稳定的。
-
公开(公告)号：US4885172A
公开(公告)日：1989-12-05
申请号：US941913
申请日：1986-12-15
CPC分类号： A61K9/1271 , A61K47/48238 , A61K47/48815 , A61K47/48823
摘要： The present invention describes a composition consisting of liposomes covalently or non-covalently coupled to the glycoprotein streptavidin. The streptavidin may additionally be coupled to biotinated proteins such as Immunoglobulin G or monoclonal antibodies.The liposomes of the invention may have a transmembrane potential across their membranes, and may be dehydrated. In addition, the composition may contain ionizable bioactive agents such as antineoplastic agents, and may be used in diagnostic assays.
摘要翻译： 本发明描述由与糖蛋白链霉抗生物素蛋白共价或非共价偶联的脂质体组成的组合物。 链霉亲和素可以另外与生物素化蛋白例如免疫球蛋白G或单克隆抗体偶联。 本发明的脂质体可跨过其膜具有跨膜电位，并且可以脱水。 此外，组合物可以含有可离子化的生物活性剂，例如抗肿瘤剂，并且可以用于诊断测定。
-
公开(公告)号：US20100041152A1
公开(公告)日：2010-02-18
申请号：US12404837
申请日：2009-03-16
CPC分类号： C12N15/88 , A61K9/1272 , Y10S436/829 , Y10S514/851 , Y10S977/80 , Y10S977/907
摘要： Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stable.
摘要翻译： 描述了可用于体外或体内转染细胞的质粒 - 脂质颗粒。 可以使用洗涤剂透析方法或利用有机溶剂的方法形成颗粒。 颗粒通常为65-85nm，完全包封质粒并且是血清稳定的。
-
公开(公告)号：US5976567A
公开(公告)日：1999-11-02
申请号：US660025
申请日：1996-06-06
申请人： Jeffery J. Wheeler , Marcel B. Bally , Yuan-Peng Zhang , Dorothy L. Reimer , Michael Hope , Pieter R. Cullis , Peter Scherrer
发明人： Jeffery J. Wheeler , Marcel B. Bally , Yuan-Peng Zhang , Dorothy L. Reimer , Michael Hope , Pieter R. Cullis , Peter Scherrer
CPC分类号： A61K47/48815 , A61K9/1272 , C12N15/88
摘要： Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or an organic solvent) the lipid-nucleic acid complexes form particles wherein the nucleic acid is serum-stable and is protected from degradation. The particles thus formed have access to extravascular sites and target cell populations and are suitable for the therapeutic delivery of nucleic acids.
-
19.发明授权
公开(公告)号：US5780054A
公开(公告)日：1998-07-14
申请号：US588014
申请日：1996-01-17
申请人： Paul G. Tardi , Erik Swartz , Marcel B. Bally , Pieter R. Cullis
发明人： Paul G. Tardi , Erik Swartz , Marcel B. Bally , Pieter R. Cullis
IPC分类号： A61K9/127
CPC分类号： A61K9/1271 , Y10S436/829
摘要： Methods of increasing the circulation half-life of protein-based therapeutics in a host, the methods comprising: (a) administering to the host an amount of a first liposome formulation comprising liposomes and an antineoplastic agent; and (b) administering to the host a second formulation comprising the protein-based therapeutic, wherein the amount of the first liposome formulation is sufficient to suppress an immune response to the protein-based therapeutic of the second formulation, thereby increasing the circulation half-life of the protein-based therapeutic.
摘要翻译： 增加宿主中基于蛋白质的治疗剂的循环半衰期的方法，所述方法包括：（a）向宿主施用一定量的包含脂质体和抗肿瘤剂的第一脂质体制剂; 并且（b）向宿主施用包含基于蛋白质的治疗剂的第二制剂，其中第一脂质体制剂的量足以抑制对第二制剂的基于蛋白质的治疗的免疫应答，从而增加循环半衰期， 基于蛋白质的治疗的生命。
-
公开(公告)号：US5981501A
公开(公告)日：1999-11-09
申请号：US484282
申请日：1995-06-07
CPC分类号： C12N15/88 , A61K9/1272 , Y10S436/829 , Y10S514/851 , Y10S977/80 , Y10S977/907
摘要： Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stable.
-
-
-
-
-
-
-
-
-
搜索结果
60 条记录 (耗时 0.035 秒)
