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公开(公告)号：US20230183702A1
公开(公告)日：2023-06-15
申请号：US18152504
申请日：2023-01-10
IPC分类号： C12N15/113 , C12N15/10 , A61K31/712
CPC分类号： C12N15/113 , A61K31/712 , C12N15/1024
摘要： The invention provides a single-stranded oligonucleotide represented by the formula [Xz — Lx]m — X — Y — [Ly — Yz]n, wherein X is represented by Xa-Xb, Xa is coupled with Y, and Xb and Y hybridize. Xa is composed of 1 to 40 nucleotides and contains at least one modified-nucleotide. Xb is composed of 4 to 40 nucleotides and contains at least one modified-nucleotide. Y is composed of 4 to 40 nucleotides and contains at least one ribonucleotide. Xz and Yz are composed of 5 to 40 nucleotides and contain at least one modified-nucleotide. Nucleotide sequences X, Xz and Yz have an antisense sequence capable of hybridizing with a target RNA. Lx and Ly are composed of 0 to 20 nucleotides.
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公开(公告)号：US20190153498A1
公开(公告)日：2019-05-23
申请号：US16091335
申请日：2017-04-04
摘要： The present invention provides a production method of a protein including suspension culture of a cell having protein production ability in a medium composition containing nanofiber under physical disruption conditions and in a state of being attached to the nanofiber.
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公开(公告)号：US20190119683A1
公开(公告)日：2019-04-25
申请号：US16073114
申请日：2017-01-26
IPC分类号： C12N15/113
摘要： Provided is a single-stranded oligonucleotide that is capable of controlling a target gene with high efficiency and can be easily produced. The single-stranded oligonucleotide is represented by the formula X-L-Y wherein X and Y hybridize by a first nucleotide sequence portion and a second nucleotide sequence portion. X is composed of 7 to 100 nucleotides, contains at least one modified nucleotide, and has a first nucleotide sequence that is capable of hybridizing with a second oligonucleotide and contains at least four contiguous nucleotides recognized by RNase H. Y is composed of 4 to 100 nucleotides, and has a second nucleotide sequence that is capable of hybridizing with a second oligonucleotide and contains at least one ribonucleotide. At least one of nucleotide sequence X and nucleotide sequence Y has an antisense sequence capable of hybridizing with a target RNA. L is a group derived from a third oligonucleotide that is degraded under physiological conditions.
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公开(公告)号：US20240084296A1
公开(公告)日：2024-03-14
申请号：US18162942
申请日：2023-02-01
IPC分类号： C12N15/113 , A61K31/7088
CPC分类号： C12N15/113 , A61K31/7088 , C12N2310/141 , C12N2310/315 , C12N2310/3519 , C12N2310/531
摘要： The invention provides a single-stranded oligonucleotide represented by the formula (I), wherein X and Y hybridize by a first nucleotide sequence portion and a second nucleotide sequence portion. X is composed of 7 to 100 nucleotides, contains at least one modified-nucleotide, and has a first nucleotide sequence capable of hybridizing with a second oligonucleotide. Y is composed of 4 to 100 nucleotides, enables hybridization with the above-mentioned first oligonucleotide, and has a second nucleotide sequence containing at least one ribonucleotide. At least one of the nucleotide sequences X, Xz and Y has an antisense sequence capable of hybridizing with a target RNA. At least one of L, Lx and Ly is a linking group that contains a non-nucleotide structure.
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公开(公告)号：US20210254055A1
公开(公告)日：2021-08-19
申请号：US16484064
申请日：2018-02-06
IPC分类号： C12N15/113 , A61K31/7088
摘要： The invention provides a single-stranded oligonucleotide represented by the formula (I), wherein X and Y hybridize by a first nucleotide sequence portion and a second nucleotide sequence portion. X is composed of 7 to 100 nucleotides, contains at least one modified-nucleotide, and has a first nucleotide sequence capable of hybridizing with a second oligonucleotide. Y is composed of 4 to 100 nucleotides, enables hybridization with the above-mentioned first oligonucleotide, and has a second nucleotide sequence containing at least one ribonucleotide. At least one of the nucleotide sequences X, Xz and Y has an antisense sequence capable of hybridizing with a target RNA. At least one of L, Lx and Ly is a linking group that contains a non-nucleotide structure.
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公开(公告)号：US20210054377A1
公开(公告)日：2021-02-25
申请号：US16982448
申请日：2019-03-20
发明人： Yoshiaki MASAKI , Kohji SEIO , Atsushi INOUE , Yusuke IRIYAMA , Tatsuro KANAKI , Hiroyuki NAKAJIMA
IPC分类号： C12N15/113 , A61K31/7125
摘要： The invention provides an antisense oligonucleotide reduced in toxicity. The antisense oligonucleotide has a central region, a 5′-side region and a 3′-side region, wherein the central region has a nucleotide (2′-3′ bridged nucleotide) in which the 2′-position and the 3′-position of a sugar moiety are bridged and/or a non-bridged nucleotide (3′-position-modified non-bridged nucleotide) having a substituent at the 3′-position.
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公开(公告)号：US20200277575A1
公开(公告)日：2020-09-03
申请号：US16764381
申请日：2018-11-16
发明人： Tatsuro KANAKI
IPC分类号： C12N5/077
摘要： The present invention provides a method for producing a unilocular adipocyte including inducing differentiation into unilocular adipocytes of mesenchymal cells having differentiation potency into adipocytes by culturing the mesenchymal cells in suspension in a liquid medium composition capable of culturing cells or tissues in suspension, wherein the liquid medium composition contains a polymer compound having an anionic functional group that binds via a divalent metal cation to form a structure capable of suspending cells or tissues, and the method wherein the polymer compound is polysaccharide, preferably polysaccharide containing a glucuronic acid moiety, more preferably deacylated gellan gum, diutan gum or xanthan gum or a salt thereof.
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公开(公告)号：US20190264158A1
公开(公告)日：2019-08-29
申请号：US16319809
申请日：2017-07-21
摘要： A method for producing a liquid medium composition, including mixing a first liquid A1 containing a particular compound and a second liquid B1 containing a linking substance, and the method is carried out using a production apparatus. The production apparatus has a container 1 provided with at least two openings 2, 3 in a wall, the two openings are connected to each other by a tube 4 outside the container, and the tube has a part with a shape and flexibility that render the tube placeable as a pumping tube into a peristaltic pump 5. In the production method, the peristaltic pump is operated, and the first liquid and the second liquid are mixed by circulating them to form the object liquid medium composition in the container.
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