公开(公告)号：US11781966B2

公开(公告)日：2023-10-10

申请号：US17289605

申请日：2019-07-05

Applicant: Nanjing University of Science and Technology

Inventor： Chao Zuo ,  Qian Chen ,  Jiaji Li ,  Jiasong Sun ,  Yao Fan ,  Shijie Feng ,  Yuzhen Zhang

IPC: G01N15/14

CPC classification number: G01N15/1434 ,  G01N15/1468 ,  G01N2015/145 ,  G01N2015/1445

Abstract: The present invention discloses a three-dimensional diffraction tomography microscopy imaging method based on LED array coded illumination. Firstly, acquiring the raw intensity images, three sets of intensity image stacks are acquired at different out-of-focus positions by moving the stage or using electrically tunable lens. And then, after acquiring the intensity image stacks of the object to be measured at different out-of-focus positions, the three-dimensional phase transfer function of the microscopy imaging system with arbitrary shape illumination is derived. Further, the three-dimensional phase transfer function of the microscopic system under circular and annular illumination with different coherence coefficients is obtained as well, and the three-dimensional quantitative refractive index is reconstructed by inverse Fourier transform of the three-dimensional scattering potential function. The scattering potential function is converted into the refractive index distribution. Thus, the quantitative three-dimensional refractive index distribution of the test object is obtained. The invention realizes high-resolution and high signal-to-noise ratio 3D diffraction tomography microscopic imaging of cells, tiny biological tissues and other samples.

公开(公告)号：US11650406B2

公开(公告)日：2023-05-16

申请号：US17294019

申请日：2019-07-05

Applicant: NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Chao Zuo ,  Qian Chen ,  Jiasong Sun ,  Yuzhen Zhang ,  Guohua Gu

IPC: G02B21/36 ,  G02B27/12 ,  H04N5/225

CPC classification number: G02B21/367 ,  G02B27/126 ,  H04N5/2256

Abstract: A microscopic imaging method of phase contrast (PC) and differential interference contrast (DIC) based on the transport of intensity equation (TIE) includes capturing three intensity images along the optical axis; solving the TIE by deconvolution to obtain the quantitative phase; obtaining the intensity image under the DIC imaging mode according to the DIC imaging principle; and obtaining the corresponding phase image of PC imaging mode according to the PC imaging principle. The method can endow the bright-field microscope with the ability to realize PC and DIC imaging without complex modification of the traditional bright-field microscope. In addition, it has the same imaging performance as the phase contrast microscope and differential interference contrast microscope, which are expensive, complex-structure, and has strict environmental conditions.

公开(公告)号：US20210372916A1

公开(公告)日：2021-12-02

申请号：US17289605

申请日：2019-07-05

Applicant: Nanjing University of Science and Technology

Inventor： Chao Zuo ,  Qian Chen ,  Jiaji Li ,  Jiasong Sun ,  Yao Fan ,  Shijie Feng ,  Yuzhen Zhang

IPC: G01N15/14

Abstract: The present invention discloses a three-dimensional diffraction tomography microscopy imaging method based on LED array coded illumination. Firstly, acquiring the raw intensity images, three sets of intensity image stacks are acquired at different out-of-focus positions by moving the stage or using electrically tunable lens. And then, after acquiring the intensity image stacks of the object to be measured at different out-of-focus positions, the three-dimensional phase transfer function of the microscopy imaging system with arbitrary shape illumination is derived. Further, the three-dimensional phase transfer function of the microscopic system under circular and annular illumination with different coherence coefficients is obtained as well, and the three-dimensional quantitative refractive index is reconstructed by inverse Fourier transform of the three-dimensional scattering potential function. The scattering potential function is converted into the refractive index distribution. Thus, the quantitative three-dimensional refractive index distribution of the test object is obtained. The invention realizes high-resolution and high signal-to-noise ratio 3D diffraction tomography microscopic imaging of cells, tiny biological tissues and other samples.

公开(公告)号：US11156821B2

公开(公告)日：2021-10-26

申请号：US16496676

申请日：2018-02-26

Applicant: NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Qian Chen ,  Chao Zuo ,  Jiasong Sun ,  Shijie Feng ,  Yuzhen Zhang ,  Guohua Gu

IPC: G02B21/06 ,  G02B21/02 ,  G02B21/08 ,  G02B21/33 ,  G02B21/36 ,  H04N5/235 ,  H04N5/361 ,  H04N9/47

Abstract: A high-illumination numerical aperture-based large field-of-view high-resolution microimaging device, and a method for iterative reconstruction, the device comprising an LED array (1), a stage (2), a condenser (3), a microscopic objective (5), a tube lens (6), and a camera (7), the LED array (1) being arranged on the forward focal plane of the condenser (3). Light emitted by the i-th lit LED unit (8) of the LED array (1) passes through the condenser (3) and converges to become parallel light illuminating a specimen (4) to be examined, which is placed on the stage (2); part of the diffracted light passing through the specimen (4) is collected by the microscopic objective (5), converged by the tube lens (6), and reaches the imaging plane of the camera (7), forming an intensity image recorded by the camera (1). The present device and method ensure controllable programming of the illumination direction, while also ensuring an illumination-numerical-aperture up to 1.20 and thus achieving a reconstruction resolution up to 0.15 μm.