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公开(公告)号：US11893719B2
公开(公告)日：2024-02-06
申请号：US17766088
申请日：2020-08-18
Applicant: NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Qian Chen , Yao Fan , Chao Zuo , Jiasong Sun , Xiangpeng Pan , Shijie Feng , Yuzhen Zhang , Guohua Gu , Jiaji Li , Jialin Zhang
CPC classification number: G06T5/50 , G02B21/367
Abstract: A single-shot differential phase contrast quantitative phase imaging method based on color multiplexing illumination. A color multiplexing illumination solution is used to realize single-shot differential phase contrast quantitative phase imaging. In the single-shot color multiplexing illumination solution, three illumination wavelengths of red, green, and blue are used to simultaneously illuminate a sample, and the information of the sample in multiple directions is converted into intensity information on different channels of a color image. By performing channel separation on this color image, the information about the sample at different spatial frequencies can be obtained. Such a color multiplexing illumination solution requires only one acquired image, thus enhancing the transfer response of the phase transfer function of single-shot differential phase contrast imaging in the entire frequency range, and achieving real-time dynamic quantitative phase imaging with a high contrast, a high resolution, and a high stability. In addition, an alternate illumination strategy is provided, so that a completely isotropic imaging resolution at the limit acquisition speed of the camera can be achieved.
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公开(公告)号：US11487096B2
公开(公告)日：2022-11-01
申请号：US17294322
申请日：2019-07-05
Applicant: Nanjing University of Science and Technology
Inventor： Qian Chen , Chao Zuo , Yao Fan , Jiasong Sun , Jiaji Li , Shijie Feng , Yuzhen Zhang
Abstract: The patent discloses a differential phase contrast (DPC) quantitative phase microscopy method based on the optimal illumination pattern design. Firstly, the optimal illumination pattern corresponding to the isotropic phase transfer function of DPC quantitative phase imaging is derived, which is determined as a semi-annular illumination pattern with the illumination numerical aperture NAill equal to the numerical aperture NAobj of the objective lens. The illumination intensity distribution varies with the cosine of the illumination angle, and it can be expressed as S(θ)=cos(θ). This patent effectively compensates for the frequency loss of phase transfer, not only the high-frequency responses of PTF are enhanced, but also the transfer responses of low-frequency phase information is significantly improved. As a result, the optimal illumination scheme ensures the correctness and achieves high resolution phase reconstruction, while the number of illuminations is reduced to a minimum of two, which greatly increases the imaging speed, allowing for real-time dynamic, high-correctness, high-resolution phase imaging results.
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3.发明授权公开(公告)号：US11781966B2
公开(公告)日：2023-10-10
申请号：US17289605
申请日：2019-07-05
Applicant: Nanjing University of Science and Technology
Inventor： Chao Zuo , Qian Chen , Jiaji Li , Jiasong Sun , Yao Fan , Shijie Feng , Yuzhen Zhang
IPC: G01N15/14
CPC classification number: G01N15/1434 , G01N15/1468 , G01N2015/145 , G01N2015/1445
Abstract: The present invention discloses a three-dimensional diffraction tomography microscopy imaging method based on LED array coded illumination. Firstly, acquiring the raw intensity images, three sets of intensity image stacks are acquired at different out-of-focus positions by moving the stage or using electrically tunable lens. And then, after acquiring the intensity image stacks of the object to be measured at different out-of-focus positions, the three-dimensional phase transfer function of the microscopy imaging system with arbitrary shape illumination is derived. Further, the three-dimensional phase transfer function of the microscopic system under circular and annular illumination with different coherence coefficients is obtained as well, and the three-dimensional quantitative refractive index is reconstructed by inverse Fourier transform of the three-dimensional scattering potential function. The scattering potential function is converted into the refractive index distribution. Thus, the quantitative three-dimensional refractive index distribution of the test object is obtained. The invention realizes high-resolution and high signal-to-noise ratio 3D diffraction tomography microscopic imaging of cells, tiny biological tissues and other samples.
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4.发明申请公开(公告)号：US20210372916A1
公开(公告)日：2021-12-02
申请号：US17289605
申请日：2019-07-05
Applicant: Nanjing University of Science and Technology
Inventor： Chao Zuo , Qian Chen , Jiaji Li , Jiasong Sun , Yao Fan , Shijie Feng , Yuzhen Zhang
IPC: G01N15/14
Abstract: The present invention discloses a three-dimensional diffraction tomography microscopy imaging method based on LED array coded illumination. Firstly, acquiring the raw intensity images, three sets of intensity image stacks are acquired at different out-of-focus positions by moving the stage or using electrically tunable lens. And then, after acquiring the intensity image stacks of the object to be measured at different out-of-focus positions, the three-dimensional phase transfer function of the microscopy imaging system with arbitrary shape illumination is derived. Further, the three-dimensional phase transfer function of the microscopic system under circular and annular illumination with different coherence coefficients is obtained as well, and the three-dimensional quantitative refractive index is reconstructed by inverse Fourier transform of the three-dimensional scattering potential function. The scattering potential function is converted into the refractive index distribution. Thus, the quantitative three-dimensional refractive index distribution of the test object is obtained. The invention realizes high-resolution and high signal-to-noise ratio 3D diffraction tomography microscopic imaging of cells, tiny biological tissues and other samples.
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