公开(公告)号：US20240101991A1

公开(公告)日：2024-03-28

申请号：US17936076

申请日：2022-09-28

Applicant: NOVOZYMES A/S

Inventor： Marc Dominique Morant ,  Tomoko Matsui ,  Kirk Matthew Schnorr ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Zhengfang Kang

IPC: C12N9/26 ,  C12N15/81 ,  C12P7/06

CPC classification number: C12N9/2408 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01028

Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.

公开(公告)号：US11377648B2

公开(公告)日：2022-07-05

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12N15/52 ,  C12N15/62 ,  C12N15/82 ,  C12N1/16

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US20210139875A1

公开(公告)日：2021-05-13

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P19/14 ,  C12P19/02 ,  C12N15/82 ,  C12P7/06

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US10584324B2

公开(公告)日：2020-03-10

申请号：US15520567

申请日：2015-10-23

Applicant: NOVOZYMES A/S

Inventor： Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/34 ,  C12P19/14 ,  C12N15/56 ,  C12N1/15 ,  C12P19/02 ,  C12P7/06 ,  C12N9/30 ,  C12N9/44 ,  C12P7/14

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10550374B2

公开(公告)日：2020-02-04

申请号：US14787418

申请日：2014-04-29

Applicant: NOVOZYMES A/S

Inventor： Noriko Tsutsumi ,  Keiichi Ayabe ,  Siik Kishishita

IPC: C12N9/34 ,  C12P19/20 ,  C12P19/02

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20150031091A1

公开(公告)日：2015-01-29

申请号：US14348433

申请日：2012-09-29

Applicant: NOVOZYMES A/S

Inventor： Ming Li ,  Junxin Duan ,  Noriko Tsutsumi ,  Guillermo Coward-Kelly ,  Henrik Lundkvist

IPC: C12N9/24 ,  A21D8/04 ,  D06M16/00 ,  C12C5/00 ,  C12P19/14 ,  C12P7/06

CPC classification number: C12N9/2402 ,  A21D8/042 ,  C12C5/004 ,  C12N9/2408 ,  C12N9/242 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01001 ,  D06L1/14 ,  D06L4/40 ,  D06M16/003 ,  D21H17/28 ,  Y02E50/17

Abstract: The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.

Abstract translation: 本发明提供了具有α-淀粉酶活性的分离的多肽催化结构域，碳水化合物结合结构域和编码多肽，催化结构域或碳水化合物结合结构域的多核苷酸。 本发明还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽，催化结构域或碳水化合物结合结构域的方法。

公开(公告)号：US11584783B2

公开(公告)日：2023-02-21

申请号：US16624164

申请日：2018-06-26

Applicant: NOVOZYMES A/S

Inventor： Marc Dominique Morant ,  Tomoko Matsui ,  Kirk Matthew Schnorr ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Zhengfang Kang

IPC: C12P7/06 ,  C12P19/14 ,  C07K14/39 ,  C12N9/24

Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.

公开(公告)号：US20170240873A1

公开(公告)日：2017-08-24

申请号：US15587576

申请日：2017-05-05

Applicant: NOVOZYMES A/S

Inventor： Ming Li ,  Junxin Duan ,  Noriko Tsutsumi ,  Guillermo Coward-Kelly ,  Henrik Lundkvist

IPC: C12N9/30 ,  A21D8/04 ,  C12P7/14

CPC classification number: C12N9/242 ,  A21D8/042 ,  C12Y302/01001

Abstract: The present invention relates to isolated polypeptides having alpha-amylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.