公开(公告)号：US08859255B2

公开(公告)日：2014-10-14

申请号：US13795180

申请日：2013-03-12

Applicant: Novozymes A/S

Inventor： Carsten Andersen ,  Christel Thea Jorgensen ,  Henrik Bisgaard-Frantzen ,  Allan Svendsen ,  Soren Kjaerulff

IPC: C12N9/00 ,  C12N9/28 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12P19/14

CPC classification number: C12N9/2417 ,  C07K2319/00 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/17 ,  Y02P20/52

Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

Abstract translation: 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

公开(公告)号：US20130178408A1

公开(公告)日：2013-07-11

申请号：US13795180

申请日：2013-03-12

Applicant: NOVOZYMES A/S

Inventor： Carsten Andersen ,  Christel Thea Jorgensen ,  Henrik Bisgaard-Frantzen ,  Allan Svendsen ,  Soren Kjaerulff

IPC: C12N9/24

CPC classification number: C12N9/2417 ,  C07K2319/00 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/17 ,  Y02P20/52

Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

公开(公告)号：US20190024067A1

公开(公告)日：2019-01-24

申请号：US16139260

申请日：2018-09-24

Applicant: NOVOZYMES A/S

Inventor： Carsten Andersen ,  Christel Thea Jorgensen ,  Henrik Bisgaard-Frantzen ,  Allan Svendsen ,  Soren Kjaerulff

IPC: C12N9/28 ,  C12P19/14 ,  C12P19/04

Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

公开(公告)号：US20170002340A1

公开(公告)日：2017-01-05

申请号：US15273227

申请日：2016-09-22

Applicant: NOVOZYMES A/S

Inventor： Carsten Andersen ,  Christel Thea Jorgensen ,  Henrik Bisgaard-Frantzen ,  Allan Svendsen ,  Soren Kjaerulff

IPC: C12N9/28 ,  C12P19/04 ,  C12P19/14

CPC classification number: C12N9/2417 ,  C07K2319/00 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/17 ,  Y02P20/52

Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

Abstract translation: 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

公开(公告)号：US20140322795A1

公开(公告)日：2014-10-30

申请号：US14330747

申请日：2014-07-14

Applicant: Novozymes A/S

Inventor： Carsten Andersen ,  Christel Thea Jorgensen ,  Henrik Bisgaard-Frantzen ,  Allan Svendsen ,  Soren Kjaerulff

IPC: C12N9/28

CPC classification number: C12N9/2417 ,  C07K2319/00 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/17 ,  Y02P20/52

Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

Abstract translation: 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

公开(公告)号：US20130252850A1

公开(公告)日：2013-09-26

申请号：US13898146

申请日：2013-05-20

Applicant: NOVOZYMES A/S

Inventor： Allan Svendsen ,  Soren Kjaerulff ,  Henrik Bisgaard-Frantzen ,  Carsten Andersen

IPC: C12N9/24

CPC classification number: C12N9/2414 ,  C07H21/04 ,  C11D3/38609 ,  C11D3/38618 ,  C12N9/2417

Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.