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公开(公告)号:US09464279B2
公开(公告)日:2016-10-11
申请号:US14330747
申请日:2014-07-14
申请人: Novozymes A/S
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgaard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
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公开(公告)号:US20160222365A1
公开(公告)日:2016-08-04
申请号:US15012229
申请日:2016-02-01
申请人: NOVOZYMES A/S
发明人: Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号: C12N9/28
摘要: The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译: 本发明涉及相对于所述亲本α-淀粉酶显示出至少一种以下特性的母体类戊酰样α-淀粉酶的变体:i)在8至10.5的pH下改善的pH稳定性; 和/或ii)在pH8至10.5时提高的Ca 2+稳定性,和/或iii)在10至60℃的温度下提高比活性。
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公开(公告)号:US20140030767A1
公开(公告)日:2014-01-30
申请号:US13907024
申请日:2013-05-31
申请人: Novozymes A/S
CPC分类号: C12N9/2417 , C11D3/386 , C11D3/38618 , C11D3/38681 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体(突变体),该变体具有α-淀粉酶活性并且表现出改变的稳定性,特别是在高温和/或低pH相对和/或低Ca 2+下 亲本α-淀粉酶。
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公开(公告)号:US20150099284A1
公开(公告)日:2015-04-09
申请号:US14572409
申请日:2014-12-16
申请人: NOVOZYMES A/S
CPC分类号: C12N9/2417 , C11D3/386 , C11D3/38618 , C11D3/38681 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体(突变体),该变体具有α-淀粉酶活性并且表现出改变的稳定性,特别是在高温和/或低pH相对和/或低Ca 2+下 亲本α-淀粉酶。
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公开(公告)号:US20140017715A1
公开(公告)日:2014-01-16
申请号:US13785190
申请日:2013-03-05
申请人: NOVOZYMES A/S
发明人: Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号: C12N9/28
摘要: The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译: 本发明涉及亲本的类戊酰样α-淀粉酶的变体,其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变:i)在8至10.5的pH下改善的pH稳定性; 和/或ii)在pH8至10.5时提高的Ca 2+稳定性,和/或iii)在10至60℃的温度下提高比活性。
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公开(公告)号:US10113157B2
公开(公告)日:2018-10-30
申请号:US15273227
申请日:2016-09-22
申请人: NOVOZYMES A/S
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgaard-Frantzen , Allan Svendsen , Soren Kjaerulff
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
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公开(公告)号:US20140248685A1
公开(公告)日:2014-09-04
申请号:US14163585
申请日:2014-01-24
申请人: NOVOZYMES A/S
发明人: Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号: C12N9/28
摘要: The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译: 本发明涉及相对于所述亲本α-淀粉酶显示出至少一种以下特性的母体类戊酰样α-淀粉酶的变体:i)在8至10.5的pH下改善的pH稳定性; 和/或ii)在pH8至10.5时提高的Ca 2+稳定性,和/或iii)在10至60℃的温度下提高比活性。
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公开(公告)号:US12076368B2
公开(公告)日:2024-09-03
申请号:US16097072
申请日:2017-04-28
申请人: Novozymes A/S
发明人: Peter Nordkild , Soren Kjaerulff
CPC分类号: A61K38/1729 , A61K9/0053 , A61K38/04 , A61K38/26 , A61P1/16 , A61P1/18 , A61P3/00 , A61K45/06 , A61K38/04 , A61K2300/00 , A61K38/1729 , A61K2300/00 , A61K38/26 , A61K2300/00
摘要: The present invention relates to methods for treatment or prevention of liver, biliary tract, and pancreatic disorders by administering one or more defensins. Included within the scope of the disclosure is also treatment of certain metabolic disorders.
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公开(公告)号:US20160312201A1
公开(公告)日:2016-10-27
申请号:US15206986
申请日:2016-07-11
申请人: NOVOZYMES A/S
CPC分类号: C12N9/2417 , C11D3/386 , C11D3/38618 , C11D3/38681 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
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公开(公告)号:US20150132823A1
公开(公告)日:2015-05-14
申请号:US14601871
申请日:2015-01-21
申请人: NOVOZYMES A/S
CPC分类号: C12N9/2414 , C07H21/04 , C11D3/38609 , C11D3/38618 , C12N9/2417
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.
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