公开(公告)号：US10550364B2

公开(公告)日：2020-02-04

申请号：US15539241

申请日：2015-12-24

Applicant: OSAKA UNIVERSITY

Inventor： Junichi Takagi ,  Emiko Mihara ,  Akira Kikuchi

IPC: C12N5/00 ,  A61K38/00 ,  C07K19/00 ,  C12P21/02 ,  C12N15/09 ,  C07K14/00

Abstract: Provided is a novel method for producing a Wnt protein, which method comprises(1) a step of performing affinity purification targeting afamin to obtain a Wnt protein from a culture product, and/or(2) a step of performing affinity purification to obtain a Wnt protein in the form of a complex with afamin from a culture product.The method enables a Wnt protein having high Wnt activity to be produced in a simple and brief manner without use of special equipment.