公开(公告)号：US11293007B2

公开(公告)日：2022-04-05

申请号：US16565898

申请日：2019-09-10

Applicant: OSAKA UNIVERSITY

Inventor： Junichi Takagi ,  Emiko Mihara ,  Akira Kikuchi

IPC: C12N5/02 ,  C12N15/09 ,  A61K38/00 ,  C12N5/00 ,  C07K14/47 ,  C07K14/76 ,  C07K19/00 ,  C12P21/02 ,  C07K14/00

Abstract: A method for producing a Wnt protein-afamin complex, the method comprising the steps of culturing Wnt protein-expressing cells in a culture medium containing a purified afamin or a recombinant afamin or co-culturing Wnt protein-expressing cells and recombinant afamin-expressing cells or culturing cells expressing both a Wnt protein and afamin; obtaining the culture supernatant; and optionally performing affinity purification to obtain the Wnt protein-afamin complex from the culture supernatant.

公开(公告)号：US11807679B2

公开(公告)日：2023-11-07

申请号：US16650337

申请日：2018-09-26

Applicant: OSAKA UNIVERSITY

Inventor： Akira Kikuchi ,  Katsumi Fumoto ,  Hirokazu Kimura

IPC: C07K16/18 ,  A61P35/00 ,  G01N33/543 ,  G01N33/574 ,  A61K39/00

CPC classification number: C07K16/18 ,  A61P35/00 ,  G01N33/543 ,  G01N33/57423 ,  G01N33/57438 ,  A61K39/00 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/73 ,  C07K2317/76

Abstract: The purpose of the present invention is to provide an anti-CKAP4 monoclonal antibody that inhibits the binding of DKK1 and CKAP4 and exhibits an exceptional antitumor effect. Provided is an anti-CKAP4 monoclonal antibody that recognizes at least part of the 451-455 region, at least part of the 481-485 region, at least part of the 502-510 region, at least part of the 503-524 region and at least part of the 585-590 region, or at least part of the 585-592 region of the amino acid sequence (amino acid sequence of CKAP4) represented by SEQ ID NO:1 as an epitope, the anti-CKAP4 monoclonal antibody effectively inhibiting the binding of DKK1 and CKAP4, the activation of AKT of S2-CP8 cells, and the proliferative ability of S2-CP8 cells or the migratory ability of S2-CP8 cells, and exhibiting an exceptional antitumor effect. The ability to develop ELISA methods in which these anti-CKAP4 monoclonal antibodies are used furthermore makes it possible, for example, to measure the serum CKAP4 in pancreatic cancer patients, and therefore also contributes to the development of companion diagnostic agents.

公开(公告)号：US20220249538A1

公开(公告)日：2022-08-11

申请号：US17595703

申请日：2020-05-22

Applicant: OSAKA UNIVERSITY

Inventor： Akira Kikuchi ,  Shinji Matsumoto ,  Taku Yamamichi

IPC: A61K31/7105 ,  G01N33/574 ,  C12Q1/6886 ,  A61P35/00 ,  A61K31/713

Abstract: The purpose of the present invention is to provide a novel tumor treatment agent. In GREB1-positive tumors that do not exhibit sex hormone sensitivity, GREB1 is a downstream target gene of the Wnt/β-catenin signal. Through the use as an active ingredient of a substance that can inhibit GREB1 expression, the growth of GREB1-positive tumor cells that do not exhibit sex hormone sensitivity can be effectively inhibited and such a tumor can be treated.

公开(公告)号：US12071624B2

公开(公告)日：2024-08-27

申请号：US17250806

申请日：2019-09-04

Applicant: OSAKA UNIVERSITY ,  NATIONAL INSTITUTES OF BIOMEDICAL INNOVATION, HEALTH AND NUTRITION ,  NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY

Inventor： Akira Kikuchi ,  Shinji Matsumoto ,  Satoshi Obika ,  Yuya Kasahara ,  Takumi Fukumoto

IPC: C07H21/04 ,  A61P35/04 ,  C12N15/113

CPC classification number: C12N15/1137 ,  A61P35/04 ,  C12N2310/11 ,  C12N2310/315

Abstract: The purpose of the present invention is to provide an antisense oligonucleotide that targets an ARL4C molecule and exerts an antitumor effect in vivo, and a nucleic acid drug using the antisense oligonucleotide. An antisense oligonucleotide that has a base sequence consisting of at least 10 consecutive bases contained in the base sequence represented by SEQ ID NO: 1. This antisense oligonucleotide targets an ARL4C molecule and thus can inhibit the expression of ARL4C in a tumor cell in vitro and suppress the migration and proliferation thereof. When systemically administered, moreover, the antisense oligonucleotide can exert an excellent antitumor effect in vivo too.

公开(公告)号：US10550364B2

公开(公告)日：2020-02-04

申请号：US15539241

申请日：2015-12-24

Applicant: OSAKA UNIVERSITY

Inventor： Junichi Takagi ,  Emiko Mihara ,  Akira Kikuchi

IPC: C12N5/00 ,  A61K38/00 ,  C07K19/00 ,  C12P21/02 ,  C12N15/09 ,  C07K14/00

Abstract: Provided is a novel method for producing a Wnt protein, which method comprises(1) a step of performing affinity purification targeting afamin to obtain a Wnt protein from a culture product, and/or(2) a step of performing affinity purification to obtain a Wnt protein in the form of a complex with afamin from a culture product.The method enables a Wnt protein having high Wnt activity to be produced in a simple and brief manner without use of special equipment.