公开(公告)号：US11975040B2

公开(公告)日：2024-05-07

申请号：US16309957

申请日：2017-06-16

Applicant: The University of Tokyo ,  Osaka University

Inventor： Hiroaki Suga ,  Nasir Kato Bashiruddin ,  Junichi Takagi ,  Yukiko Matsunaga

IPC: A61K38/02 ,  A61K9/00 ,  A61K38/04 ,  A61P9/00 ,  A61P19/00 ,  A61P19/08 ,  A61P25/28 ,  A61P35/00 ,  A61P43/00 ,  C07K7/50

CPC classification number: A61K38/02 ,  A61K9/0019 ,  A61K9/0053 ,  A61K38/04 ,  A61P19/00 ,  A61P19/08 ,  A61P25/28 ,  A61P35/00 ,  A61P43/00 ,  C07K7/50

Abstract: The present invention provides a Plexin-binding regulating agent containing a cyclic peptide having an Arg-Trp-Thr structure or a Leu-Ser-Trp structure or a pharmaceutically acceptable salt of the cyclic peptide.

公开(公告)号：US20220259298A1

公开(公告)日：2022-08-18

申请号：US17625520

申请日：2020-07-10

Applicant: Kyoto University ,  University of Fukui ,  Osaka University

Inventor： Katsu Takahashi ,  Manabu Sugai ,  Yoshihito Tokita ,  Junichi Takagi ,  Emiko Mihara

IPC: C07K16/22 ,  A61P1/02

Abstract: Provided are: an antibody which specifically binds to and neutralizes USAG-1 or an antigen-binding fragment thereof; and a pharmaceutical composition containing the antibody or the antigen-binding fragment.

公开(公告)号：US20210317233A1

公开(公告)日：2021-10-14

申请号：US17264496

申请日：2019-07-31

Applicant: The University of Tokyo ,  Osaka University

Inventor： Hiroaki Suga ,  Junichi Takagi

IPC: C07K16/46 ,  C07K16/28

Abstract: The purpose of the present invention is to provide an efficient and versatile method for granting an additional binding activity to an antibody by inserting a peptide sequence into the antibody. The present invention provides a method of granting a binding ability to a second target molecule to an antibody, comprising inserting an internal peptide sequence of a cyclic peptide into an Fc region of the antibody, wherein the cyclic peptide has the binding ability to the second target molecule different from a first target molecule to be recognized by the antibody.

公开(公告)号：US10513562B2

公开(公告)日：2019-12-24

申请号：US16345639

申请日：2017-11-07

Applicant: FUJIFILM WAKO PURE CHEMICAL CORPORATION ,  OSAKA UNIVERSITY

Inventor： Junichi Takagi

IPC: C07K9/00 ,  C07K16/46 ,  C07K1/30

Abstract: An object of the present invention is to provide a fragment antibody which can be conveniently produced as one having antigen-binding activity, and which has a greater ability to crystallize itself alone or as a complex with an antigen molecule than that of Fv-clasp (v1) even in a case where the fragment antibody is obtained in an E. coli expression system. The present invention relates to a fragment antibody including a complex of a peptide (VH(112C)-SARAH) in which an N-terminus of a SARAH domain is linked to a C-terminus of a heavy chain domain (VH region) of an antibody, and an amino acid residue of antibody residue 112 according to Chothia numbering scheme in the VH region is mutated to cysteine; and a peptide (VL-SARAH(37C)) in which an N-terminus of a SARAH domain is linked to a C-terminus of a light chain domain (VL region) of an antibody, and an amino acid residue at position 13 from the C-terminus in the SARAH domain is mutated to cysteine.

公开(公告)号：US11293007B2

公开(公告)日：2022-04-05

申请号：US16565898

申请日：2019-09-10

Applicant: OSAKA UNIVERSITY

Inventor： Junichi Takagi ,  Emiko Mihara ,  Akira Kikuchi

IPC: C12N5/02 ,  C12N15/09 ,  A61K38/00 ,  C12N5/00 ,  C07K14/47 ,  C07K14/76 ,  C07K19/00 ,  C12P21/02 ,  C07K14/00

Abstract: A method for producing a Wnt protein-afamin complex, the method comprising the steps of culturing Wnt protein-expressing cells in a culture medium containing a purified afamin or a recombinant afamin or co-culturing Wnt protein-expressing cells and recombinant afamin-expressing cells or culturing cells expressing both a Wnt protein and afamin; obtaining the culture supernatant; and optionally performing affinity purification to obtain the Wnt protein-afamin complex from the culture supernatant.

公开(公告)号：US10988540B2

公开(公告)日：2021-04-27

申请号：US16905211

申请日：2020-06-18

Applicant: OSAKA UNIVERSITY

Inventor： Naoki Hosen ,  Haruo Sugiyama ,  Atsushi Kumanogoh ,  Junichi Takagi

IPC: C07K16/00 ,  C07K16/46 ,  C07K16/28 ,  A61K35/17 ,  C12N5/10 ,  C12N15/09 ,  A61K39/00 ,  C07K19/00 ,  A61P35/00 ,  C07K16/30

Abstract: Provided is an active ingredient of a pharmaceutical composition for treating myeloma. Specifically, provided is an antibody whose epitope is present in the region of the amino acid residue positions 20 to 109 of human integrin β7.

公开(公告)号：US20200377588A1

公开(公告)日：2020-12-03

申请号：US16635172

申请日：2018-07-31

Applicant: The University of Tokyo ,  Osaka University

Inventor： Hiroaki Suga ,  Junichi Takagi

IPC: C07K16/24 ,  C07K14/46

Abstract: The present invention provides a method of presenting a cyclic peptide on a protein having a loop structure. The cyclic peptide has a chemically crosslinked structure for forming an intramolecular cyclic structure. The method includes replacing the chemically crosslinked structure of the cyclic peptide with two amino acid residues constituting the loop structure, thereby fusing the cyclic peptide with the protein having a loop structure.

公开(公告)号：US20190247457A1

公开(公告)日：2019-08-15

申请号：US16309957

申请日：2017-06-16

Applicant: The University of Tokyo ,  Osaka University

Inventor： Hiroaki Suga ,  Nasir Kato Bashiruddin ,  Junichi Takagi ,  Yukiko Matsunaga

IPC: A61K38/02 ,  A61P25/28 ,  A61P19/00 ,  A61K9/00

CPC classification number: A61K38/02 ,  A61K9/0019 ,  A61K9/0053 ,  A61K38/04 ,  A61P19/00 ,  A61P19/08 ,  A61P25/28 ,  A61P35/00 ,  A61P43/00 ,  C07K7/50

Abstract: The present invention provides a Plexin-binding regulating agent containing a cyclic peptide having an Arg-Trp-Thr structure or a Leu-Ser-Trp structure or a pharmaceutically acceptable salt of the cyclic peptide.

公开(公告)号：US12139406B2

公开(公告)日：2024-11-12

申请号：US17441971

申请日：2020-03-27

Applicant: OSAKA UNIVERSITY

Inventor： Tsuyoshi Inoue ,  Haruyasu Asahara ,  Kei Ohkubo ,  Kenji Iwasaki ,  Naoyuki Miyazaki ,  Mika Hirose ,  Atsushi Nakagawa ,  Junichi Takagi ,  Takefumi Doi ,  Hiroaki Adachi

IPC: C01B32/156 ,  C01B32/05 ,  C01B32/168 ,  C01B32/194 ,  H01J37/20

Abstract: The present invention provides a method for producing a substance with a modified carbon allotrope surface that can suppress or prevent uneven distribution, uneven orientation, and the like of a structural analysis target substance in a structural analysis by cryo-electron microscopy. A method for producing a substance with a modified carbon allotrope surface of the present invention includes: the step of surface-treating by reacting a carbon allotrope surface with a halogen oxide radical, wherein the carbon allotrope surface is modified by the surface-treating.

公开(公告)号：US10654931B2

公开(公告)日：2020-05-19

申请号：US15751574

申请日：2016-08-02

Applicant: OSAKA UNIVERSITY

Inventor： Naoki Hosen ,  Haruo Sugiyama ,  Atsushi Kumanogoh ,  Junichi Takagi

IPC: C07K16/28 ,  A61K39/00 ,  C07K19/00 ,  C12N5/10 ,  C12N5/09 ,  A61K35/17 ,  C12N15/09 ,  A61P35/00 ,  C07K16/30

Abstract: Provided is an active ingredient of a pharmaceutical composition for treating myeloma. Specifically, provided is an antibody whose epitope is present in the region of the amino acid residue positions 20 to 109 of human integrin β7.