公开(公告)号：US11193120B2

公开(公告)日：2021-12-07

申请号：US16692355

申请日：2019-11-22

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Hai-Rong Li

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6886

Abstract: The invention provides methods for isolating RNA from the soluble fraction of urine. The methods can be used for detecting the presence or absence of an RNA, or quantifying the amount of an RNA. The methods are useful for diagnosing an individual suspected of having a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine. The methods are also useful for prognosing an individual diagnosed with a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine.

公开(公告)号：US11085079B2

公开(公告)日：2021-08-10

申请号：US16171675

申请日：2018-10-26

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Hai-Rong Li ,  Feras Hantash ,  Frederic Waldman

IPC: C12Q1/6876 ,  C12Q1/6869

Abstract: Described herein are methods, compositions and kits directed to amplification of nucleic acids suitable for both next generation sequencing (NGS) and a second round of sequencing as validation, such as Sanger sequencing.

公开(公告)号：US20200157527A1

公开(公告)日：2020-05-21

申请号：US16692355

申请日：2019-11-22

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Hai-Rong Li

IPC: C12N15/10 ,  C12Q1/6886

Abstract: The invention provides methods for isolating RNA from the soluble fraction of urine. The methods can be used for detecting the presence or absence of an RNA, or quantifying the amount of an RNA. The methods are useful for diagnosing an individual suspected of having a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine. The methods are also useful for prognosing an individual diagnosed with a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine.

公开(公告)号：US20190062726A1

公开(公告)日：2019-02-28

申请号：US16118864

申请日：2018-08-31

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Hai-Rong Li

IPC: C12N15/10 ,  C12Q1/6886

Abstract: The invention provides methods for isolating RNA from the soluble fraction of urine. The methods can be used for detecting the presence or absence of an RNA, or quantifying the amount of an RNA. The methods are useful for diagnosing an individual suspected of having a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine. The methods are also useful for prognosing an individual diagnosed with a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine.

公开(公告)号：US12264357B2

公开(公告)日：2025-04-01

申请号：US17397280

申请日：2021-08-09

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Hai-Rong Li ,  Feras Hantash ,  Frederic Waldman

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: Described herein are methods, compositions and kits directed to amplification of nucleic acids suitable for both next generation sequencing (NGS) and a second round of sequencing as validation, such as Sanger sequencing.

公开(公告)号：US11981966B2

公开(公告)日：2024-05-14

申请号：US16870033

申请日：2020-05-08

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Kevin Qu ,  James Prentice ,  Frederic Waldman

IPC: C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present technology relates to methods for determining whether a patient diagnosed with breast cancer, colorectal cancer, melanoma or lung cancer will benefit from or is predicted to be responsive to treatment with an individual therapeutic agent or a specific combination of therapeutic agents. These methods are based on screening a patient's solid tumors and detecting alterations in target nucleic acid sequences corresponding to a specific set of cancer-related genes. Kits for use in practicing the methods are also provided.

公开(公告)号：US11965156B2

公开(公告)日：2024-04-23

申请号：US17543944

申请日：2021-12-07

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Hai-Rong Li

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6886

CPC classification number: C12N15/1017 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: The invention provides methods for isolating RNA from the soluble fraction of urine. The methods can be used for detecting the presence or absence of an RNA, or quantifying the amount of an RNA. The methods are useful for diagnosing an individual suspected of having a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine. The methods are also useful for prognosing an individual diagnosed with a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine.

公开(公告)号：US11473144B2

公开(公告)日：2022-10-18

申请号：US16461356

申请日：2017-11-13

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Nigel J. Clarke

IPC: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  A61P43/00

Abstract: The present disclosure provides methods for determining whether a patient exhibiting cystic fibrosis symptoms, or a patient at risk for cystic fibrosis, will benefit from treatment with one or more anti-cystic fibrosis therapeutic agents. These methods are based on detecting hereditary cystic fibrosis related mutations in small-volume dried biological fluid samples that are collected using a volumetric absorptive microsampling device. Kits for use in practicing the methods are also provided.

公开(公告)号：US20190112657A1

公开(公告)日：2019-04-18

申请号：US16171675

申请日：2018-10-26

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Hai-Rong Li ,  Feras Hantash ,  Frederic Waldman

IPC: C12Q1/6876 ,  C12Q1/6869

Abstract: Described herein are methods, compositions and kits directed to amplification of nucleic acids suitable for both next generation sequencing (NGS) and a second round of sequencing as validation, such as Sanger sequencing.