公开(公告)号：US20210069648A1

公开(公告)日：2021-03-11

申请号：US17011304

申请日：2020-09-03

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll

IPC: B01D63/02 ,  B01J20/26 ,  B01J20/28 ,  C12M1/00 ,  B01D69/08

Abstract: The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

公开(公告)号：US20200080041A1

公开(公告)日：2020-03-12

申请号：US16687864

申请日：2019-11-19

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll

IPC: C12M1/00 ,  C12M1/26 ,  B01D69/08 ,  B01D71/48 ,  B01D71/26 ,  B01D61/18

Abstract: The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

公开(公告)号：US20190337979A1

公开(公告)日：2019-11-07

申请号：US15972442

申请日：2018-05-07

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby

IPC: C07K1/36 ,  B01D63/02 ,  B01D69/08

Abstract: The present disclosure pertains to filtration methods comprising: passing a first fluid that comprises cells, cell debris and targeted product produced by the cells through a first filter thereby separating the first fluid into a first retentate comprising cells and a first permeate comprising targeted product and cell debris; combining resin beads having affinity for targeted product with the first permeate to form a second fluid containing resin beads with bound target product and cell debris; passing the second fluid through a second filter thereby separating the second fluid into a second retentate comprising resin beads with bound target product and a second permeate comprising cell debris; combining an elution buffer with the second retentate to form a third fluid that comprises a mixture of resin beads and unbound targeted product; and passing the third fluid through a third filter thereby separating resin beads from targeted product.

公开(公告)号：US20240238731A1

公开(公告)日：2024-07-18

申请号：US18605090

申请日：2024-03-14

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll ,  Philip Yuen ,  Ralf Kuriyel

IPC: B01D63/06 ,  B01D61/14 ,  B01D63/02 ,  B01D65/08 ,  C12M1/26

CPC classification number: B01D63/06 ,  B01D61/147 ,  B01D63/02 ,  B01D65/08 ,  C12M33/14 ,  B01D2315/10 ,  B01D2321/20

Abstract: The disclosure provides tangential flow depth filtration (TFDF) systems which exhibit improved filter fluxes and process capacities and reduced fouling characteristics. The TFDF systems of the disclosure optionally utilize tubular depth filters (TDF). Methods are provided which include the passage of a non-laminar flow through at least a portion of a length of a TDF in a TFDF system.

公开(公告)号：US11946033B2

公开(公告)日：2024-04-02

申请号：US17729022

申请日：2022-04-26

Applicant: Repligen Corporation

Inventor： Michael Bransby ,  Philip Yuen

IPC: C12M1/00 ,  B01D61/14 ,  B01D61/18 ,  C12M1/26

CPC classification number: C12M29/10 ,  B01D61/147 ,  B01D61/18 ,  C12M29/04 ,  C12M33/14 ,  B01D61/146 ,  B01D2311/16 ,  B01D2315/10

Abstract: A micro alternating tangential flow (microATF) perfusion filter includes a hollow cylinder having a proximal end and a distal end. The proximal end is connected in series to a permeate chamber, followed by a retentate chamber. The proximal end either (i) terminates in or at the permeate chamber, or (ii) terminates in or at the retentate chamber. The portion of the proximal end within the permeate chamber, in a case of (ii), possesses at least one opening allowing fluid communication between an inside of the hollow cylinder and the permeate chamber. The microATF perfusion filter further includes an inlet, positioned over the retentate chamber, for communication with a source of positive or negative pressure, and an outlet, positioned in a wall of the permeate chamber, which can be connected to a check valve, which, in turn, can be connected to a hydrophobic fluid vent filter.

公开(公告)号：US11667672B2

公开(公告)日：2023-06-06

申请号：US17331963

申请日：2021-05-27

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby

IPC: C07K1/36 ,  B01D63/02 ,  B01D69/08 ,  B01D61/14 ,  B01D15/38

CPC classification number: C07K1/36 ,  B01D15/3809 ,  B01D61/146 ,  B01D63/02 ,  B01D69/08 ,  B01D2311/06 ,  B01D2311/2626 ,  B01D2315/10 ,  B01D2315/14 ,  B01D2317/022 ,  B01D2317/025 ,  B01D2311/06 ,  B01D2311/2626

Abstract: The present disclosure pertains to filtration methods comprising: passing a first fluid that comprises cells, cell debris and targeted product produced by the cells through a first filter thereby separating the first fluid into a first retentate comprising cells and a first permeate comprising targeted product and cell debris; combining resin beads having affinity for targeted product with the first permeate to form a second fluid containing resin beads with bound target product and cell debris; passing the second fluid through a second filter thereby separating the second fluid into a second retentate comprising resin beads with bound target product and a second permeate comprising cell debris; combining an elution buffer with the second retentate to form a third fluid that comprises a mixture of resin beads and unbound targeted product; and passing the third fluid through a third filter thereby separating resin beads from targeted product.

公开(公告)号：US11643628B2

公开(公告)日：2023-05-09

申请号：US17218563

申请日：2021-03-31

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll

IPC: C12M1/00 ,  C12M1/26 ,  B01D61/18 ,  B01D69/08 ,  B01D71/26 ,  B01D71/48

CPC classification number: C12M29/16 ,  B01D61/18 ,  B01D69/081 ,  B01D71/26 ,  B01D71/48 ,  C12M29/10 ,  C12M33/10 ,  B01D2315/10 ,  B01D2325/02

Abstract: The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

公开(公告)号：US20220228098A1

公开(公告)日：2022-07-21

申请号：US17713289

申请日：2022-04-05

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll

IPC: C12M1/00 ,  B01D61/18 ,  B01D71/26 ,  B01D71/48 ,  B01D69/08 ,  C12M1/26

Abstract: The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

公开(公告)号：US20220080359A1

公开(公告)日：2022-03-17

申请号：US17091616

申请日：2019-05-28

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll ,  Philip Yuen ,  Ralf Kuriyel

IPC: B01D63/06 ,  B01D61/14 ,  B01D65/08 ,  C12M1/26

Abstract: The disclosure provides tangential flow depth filtration (TFDF) systems which exhibit improved filter fluxes and process capacities and reduced fouling characteristics. The TFDF systems of the disclosure optionally utilize tubular depth filters (TDF). Methods are provided which include the passage of a non-laminar flow through at least a portion of a length of a TDF in a TFDF system.

公开(公告)号：US12220665B2

公开(公告)日：2025-02-11

申请号：US18605090

申请日：2024-03-14

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll ,  Philip Yuen ,  Ralf Kuriyel

IPC: B01D61/14 ,  B01D63/02 ,  B01D63/06 ,  B01D65/08 ,  C12M1/26

Abstract: The disclosure provides tangential flow depth filtration (TFDF) systems which exhibit improved filter fluxes and process capacities and reduced fouling characteristics. The TFDF systems of the disclosure optionally utilize tubular depth filters (TDF). Methods are provided which include the passage of a non-laminar flow through at least a portion of a length of a TDF in a TFDF system.