公开(公告)号：US20200048312A1

公开(公告)日：2020-02-13

申请号：US16663417

申请日：2019-10-25

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Mario Gloeck ,  Peter Muench ,  Alexander Riedel ,  Christian Scholz ,  Gloria Tabares

IPC: C07K14/005 ,  G01N33/569

Abstract: The disclosure concerns a polypeptide suitable for detecting antibodies against Zika virus in an isolated biological sample having a Zika virus NS1 wing domain specific amino acid sequence and variants thereof, wherein no further Zika virus specific amino acid sequences are present in the polypeptide. This polypeptide does not immunologically cross-react with antibodies raised against structurally related antigens from tick-borne encephalitis virus, Dengue virus 1-4, West Nile virus, yellow fever virus or Japanese encephalitis virus, but immunologically reacts with antibodies raised against full length Zika virus NS1 antigen. Also disclosed is a method of producing a soluble and immunoreactive Zika virus NS1 polypeptide as well as methods and kits for detecting antibodies specific for Zika virus in an isolated sample.

公开(公告)号：US20180164314A1

公开(公告)日：2018-06-14

申请号：US15892829

申请日：2018-02-09

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Peter Muench ,  Mirko Ritter ,  Gloria Tabares

IPC: G01N33/569 ,  G01N33/543

CPC classification number: G01N33/56983 ,  G01N33/54393 ,  G01N2333/015

Abstract: Disclosed is a method for detecting a capsid polypeptide of a non-enveloped virus in a sample from a subject which includes (a) contacting the sample with a base, and (b) detecting a capsid polypeptide of the virus in the sample. Moreover, the present disclosure relates to a method for pre-processing a sample from a subject for detection of a virus, including contacting the sample with a base. Moreover, the present disclosure relates to kits, uses and devices related to these methods.