公开(公告)号：US20240077474A1

公开(公告)日：2024-03-07

申请号：US17929779

申请日：2022-09-06

Applicant: Seagate Technology LLC

Inventor： James Froberg ,  Gemma Mendonsa ,  Calvin Daniel Nazareth

IPC: G01N33/531 ,  G01N33/543

CPC classification number: G01N33/531 ,  G01N33/5436

Abstract: The present disclosure describes a system and methods for positioning a configured number of biomaterials on a surface. The method disclosed includes the formation of a membrane mask structure including a configured number of nanopores. The nanopores are generally sized to be the same size as, or slightly larger than a single target biomaterial. The masking membrane is then adhered to a substrate. The substrate may include a raw substrate, or may be functionalized for the purpose of binding biomaterials with greater attraction. A solution including the target biomaterials is then exposed to the masking membrane. One biomaterial is able to adhere to the substrate through each nanopore. The solution is rinsed from the membrane surface leaving only the biomaterial that has adhered to the substrate with a relatively strong binding force. The masking membrane may then either remain or may be removed.

公开(公告)号：US11774366B2

公开(公告)日：2023-10-03

申请号：US16986108

申请日：2020-08-05

Applicant: Seagate Technology LLC

Inventor： Gemma Mendonsa ,  Eric K. Wadleigh ,  Vivek Krishnamurthy ,  Riyan A. Mendonsa ,  Martin G. Blaber ,  Krishnan Subramanian

IPC: C12Q1/6869 ,  G01N21/65 ,  C12N9/12 ,  G01N27/447 ,  B82Y30/00

CPC classification number: G01N21/658 ,  C12N9/1252 ,  C12Q1/6869 ,  G01N27/44786 ,  B82Y30/00

Abstract: A Surface-Enhanced Raman Spectroscopy (SERS) device to perform accurate label-free long-read DNA sequencing. A Raman sensor has a hot spot defined by plasmonic nanostructures and excited by at least one laser. An immobilized DNA polymerase can be used to pull a DNA template strand to be sequenced through the hot spot.

公开(公告)号：US11654436B2

公开(公告)日：2023-05-23

申请号：US17397162

申请日：2021-08-09

Applicant: Seagate Technology LLC

Inventor： Tim Rausch ,  Edward Charles Gage ,  Walter R. Eppler ,  Gemma Mendonsa

IPC: B01L7/00 ,  B01L9/00 ,  B01L3/00 ,  C12Q1/686 ,  B82Y5/00 ,  B82Y25/00

CPC classification number: B01L7/52 ,  B01L3/502715 ,  B01L9/527 ,  B01L2200/027 ,  B01L2300/087 ,  B01L2300/1816 ,  B01L2300/1866 ,  B82Y5/00 ,  B82Y25/00 ,  C12Q1/686

Abstract: A microfluidic device for polymerase chain reaction (PCR) processing includes a platform with a microstructure with at least one reaction chamber, and a heating element. The heating element heats at least a part of the at least one reaction chamber. The heating element includes at least one spin torque oscillator (STO) configured to heat the at least a part of the at least one reaction chamber to one or more temperatures for PCR processing.