公开(公告)号：US11591648B2

公开(公告)日：2023-02-28

申请号：US16591232

申请日：2019-10-02

Applicant: Seagate Technology LLC

Inventor： Gemma Mendonsa ,  Riyan A. Mendonsa ,  Krishnan Subramanian

IPC: C12Q1/68 ,  C12Q1/6869 ,  G01N27/72 ,  G01N33/487

Abstract: Method of utilizing a nanochannel in combination with at least one magnetic sensor for detecting (e.g., identifying) molecules, cells, and other analytes. Particularly, the method includes bringing molecules, labeled with magnetic nanoparticles (MNPs), in close proximity to the magnetic sensor to identify the molecules via an output signal from the magnetic sensor. The method is particularly suited for identifying nucleotides of DNA and RNA strands.

公开(公告)号：US20210148828A1

公开(公告)日：2021-05-20

申请号：US16986108

申请日：2020-08-05

Applicant: Seagate Technology LLC

Inventor： Gemma Mendonsa ,  Eric K. Wadleigh ,  Vivek Krishnamurthy ,  Riyan A. Mendonsa ,  Martin G. Blaber ,  Krishnan Subramanian

IPC: G01N21/65 ,  C12N9/12 ,  C12Q1/6869 ,  G01N27/447

Abstract: A Surface-Enhanced Raman Spectroscopy (SERS) device to perform accurate label-free long-read DNA sequencing. A Raman sensor has a hot spot defined by plasmonic nanostructures and excited by at least one laser. An immobilized DNA polymerase can be used to pull a DNA template strand to be sequenced through the hot spot.

公开(公告)号：US11990184B2

公开(公告)日：2024-05-21

申请号：US17031664

申请日：2020-09-24

Applicant: Seagate Technology LLC

Inventor： Gemma Mendonsa ,  Riyan Alex Mendonsa ,  Tim Rausch

IPC: C12P19/34 ,  G11C13/02

CPC classification number: G11C13/02 ,  C12P19/34

Abstract: Methods of writing data to a DNA strand by inserting data-encoding oligos or symbols into a DNA backbone. One particular method of synthesizing a DNA strand encoding data includes cleaving a DNA backbone into multiple segments, and pasting a plurality of data-encoding oligo symbols between the multiple segments, with the terminal ends of the segments joining homologous terminal ends of the symbols, resulting in the DNA strand encoding data comprising alternating segments and symbols.

公开(公告)号：US11686705B2

公开(公告)日：2023-06-27

申请号：US16870580

申请日：2020-05-08

Applicant: Seagate Technology LLC

Inventor： Gemma Mendonsa ,  Tim Rausch ,  Riyan Alex Mendonsa

IPC: G01N27/447 ,  C12N15/10

CPC classification number: G01N27/44791 ,  C12N15/101 ,  G01N27/4473

Abstract: Systems and methods for identifying DNA strand size and purifying the DNA based on strand size using electrophoresis. The methods include moving, via voltage, a plurality of DNA strands through a separation gel from an inlet of a capillary or passage to either a first outlet or a second outlet dependent on the DNA strand length. In some implementations, the system is a capillary electrophoresis system. In other implementations, the system is a microfluidic lab-on-a-chip.

公开(公告)号：US11066661B2

公开(公告)日：2021-07-20

申请号：US16856947

申请日：2020-04-23

Applicant: Seagate Technology LLC

Inventor： Tim Rausch ,  Walter R Eppler ,  Gemma Mendonsa

IPC: G16B50/30 ,  C12N15/10 ,  C40B60/14 ,  B01L3/00 ,  G06F12/02 ,  G11C13/02

Abstract: A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first overhanging end and a second overhanging end and a second linker of the pair having a first overhanging end and a second overhanging end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

公开(公告)号：US11643647B2

公开(公告)日：2023-05-09

申请号：US17348642

申请日：2021-06-15

Applicant: Seagate Technology LLC

Inventor： Tim Rausch ,  Walter R Eppler ,  Gemma Mendonsa

IPC: C12N15/10 ,  C40B60/14 ,  B01L3/00 ,  G16B50/30 ,  G06F12/02 ,  G11C13/02

CPC classification number: C12N15/1031 ,  B01L3/5027 ,  C12N15/1065 ,  C12N15/1093 ,  C40B60/14 ,  G06F12/02 ,  G11C13/02 ,  G16B50/30

Abstract: A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first end and a second end and a second linker of the pair having a first end and a second end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

公开(公告)号：US20230090248A1

公开(公告)日：2023-03-23

申请号：US17677932

申请日：2022-02-22

Applicant: Seagate Technology LLC

Inventor： James Froberg ,  Gemma Mendonsa

IPC: B01L3/00

Abstract: A microfluidic system includes a hydrophobic fluidic platform and a heater. The platform includes a plurality of electrode cells operably connected to a voltage source and a controller. The heater is configured to fuse first and second vesicles. The first and second vesicles encapsulate first and second DNA precursors, respectively. The fusing combines the first and second DNA precursors. In another embodiment, a microfluidic system includes a fluidic platform including a plurality of electrode cells, a vesicle mover, and a reaction facilitator. The vesicle mover is configured to move first and second vesicles to a selected cell of the plurality of electrode cells. The reaction facilitator is operably connected to the selected cell. A method includes providing a fluidic platform comprising a plurality of cells; moving first and second vesicles encapsulating first and second reagents, respectively, to a first cell; and fusing the first and second vesicles.

公开(公告)号：US20230049708A1

公开(公告)日：2023-02-16

申请号：US17869972

申请日：2022-07-21

Applicant: Seagate Technology LLC

Inventor： Riyan Alex Mendonsa ,  Gemma Mendonsa ,  Brett R. Herdendorf

IPC: B01L3/00 ,  H01F1/44 ,  H01F7/06

Abstract: A microfluidic system includes a fluidic platform having a surface, a first liquid disposed onto the fluidic platform, and a droplet disposed onto the first liquid. The first liquid has a first temperature. The droplet has a second temperature higher than the first temperature so that the droplet is levitated above the first liquid by a cushion of vapor of the first liquid. In an embodiment, a device is configured to provide a magnetic field that has variable strength across the surface. A location of a magnetic droplet relative to the surface area is affected by the magnetic field. A method includes providing a fluidic platform, providing a magnetic field, introducing a first liquid onto the fluidic platform, introducing a first magnetic droplet onto the first liquid, and locally varying the magnetic field.

公开(公告)号：US20240336966A1

公开(公告)日：2024-10-10

申请号：US18607991

申请日：2024-03-18

Applicant: Seagate Technology LLC

Inventor： Vivek Krishnamurthy ,  Aditya Jain ,  Gemma Mendonsa ,  Anil J. Reddy

IPC: C12Q1/6869

CPC classification number: C12Q1/6869

Abstract: A laser-based molecular sequencing system includes a two-dimensional photonic crystal surface emitting laser (PCSEL) array including a plurality of PCSEL devices located in a first layer, each PCSEL device oriented in a direction perpendicular to the first layer. The system also includes a controller configured to modulate a bias on at least one PCSEL device of the plurality of PCSEL devices, the modulation configured to selectively operate each PCSEL device as an emitter or detector at a point in time based on the bias.

公开(公告)号：US11939570B2

公开(公告)日：2024-03-26

申请号：US16857006

申请日：2020-04-23

Applicant: Seagate Technology LLC

Inventor： Tim Rausch ,  Walter R Eppler ,  Gemma Mendonsa

IPC: C12N15/10 ,  B01L3/00 ,  C40B60/14 ,  G06F12/02 ,  G11C13/02 ,  G16B50/30

CPC classification number: C12N15/1031 ,  B01L3/5027 ,  C12N15/1065 ,  C12N15/1093 ,  C40B60/14 ,  G06F12/02 ,  G11C13/02 ,  G16B50/30

Abstract: A microfluidic lab-on-a-chip system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The lab-on-a-chip has a fluidic platform with a plurality of arrays operably connected to a voltage source and a controller for the voltage source, a set of first inlets operably connected to the fluidic platform, each first inlet for one DNA symbol from a DNA symbol library, a set of second inlets operably connected to the fluidic platform, each second inlet for one DNA linker from a DNA linker library, and a mixing area operably connected to the fluidic platform and to the plurality of first inlets and the plurality of second inlets.