公开(公告)号：US20050049308A1

公开(公告)日：2005-03-03

申请号：US10899155

申请日：2004-07-27

申请人： Shunichi Akiba ,  Katsutoshi Ara ,  Hiroshi Kusuoku ,  Yoshinori Nishizawa

发明人： Shunichi Akiba ,  Katsutoshi Ara ,  Hiroshi Kusuoku ,  Yoshinori Nishizawa

IPC分类号： A61P25/00 ,  A61K31/19 ,  A61K31/202 ,  A61P25/18 ,  A61P25/28 ,  A61P43/00

CPC分类号： A61K31/202

摘要： The invention contemplates drugs useful for maintenance or promotion at sites of regenerating nerves and for prevention or treatment of pathological conditions caused by denaturation or cell damage in the nervous system. Thus, the invention provides an apolipoprotein D degradation inhibitor containing lauric acid or oleic acid as an active ingredient.

摘要翻译： 本发明考虑了用于在再生神经的部位进行维持或促进的药物和用于预防或治疗由神经系统变性或细胞损伤引起的病理状况的药物。 因此，本发明提供含有月桂酸或油酸作为活性成分的载脂蛋白D降解抑制剂。

公开(公告)号：US20110014709A1

公开(公告)日：2011-01-20

申请号：US12933766

申请日：2009-03-23

申请人： Katsutoshi Ara ,  Takuya Morimoto ,  Naotake Ogasawara

发明人： Katsutoshi Ara ,  Takuya Morimoto ,  Naotake Ogasawara

IPC分类号： C12N15/87 ,  C12N15/63

CPC分类号： C12N15/1082 ,  C12N15/102

摘要： A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5′-side region outside of the target region in the host DNA, a 3′-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3′-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5′-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3′-side region derived from the host DNA and the 3′-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter: and a selectable marker cassette for use in the method.

摘要翻译： 使用供体DNA修饰宿主DNA中的靶区域的方法：其中具有与宿主DNA中的靶区域外侧的5'-侧区域同源的区域的供体DNA，靶标外的3'侧区域 区域，宿主DNA中的靶区域内的第一同源重组区域，并且还具有第一选择标记基因，表达诱导启动子和第二选择标记基因 控制与3'侧区域同源的区域与与第一同源重组区域同源的区域之间的表达诱导启动子; 该方法具有以下步骤：在5'-侧区域和第一同源重组区域的区域上进行供体DNA和宿主DNA之间的同源重组的第一步骤，以进行与供体DNA整合的宿主的选择 基于第一选择标记基因的表达; 以及在源自宿主DNA的3'-侧区域的两个区域和源自供体DNA的3'侧区域之间，通过第1步在与供体DNA整合的宿主DNA内进行同源重组的第2步骤 ，用于在表达诱导启动子的表达诱导条件下，基于第二选择标记基因的表达来选择其目标区域被修饰的宿主;以及用于该方法的可选择标记盒。

公开(公告)号：US06338959B1

公开(公告)日：2002-01-15

申请号：US09514302

申请日：2000-02-28

申请人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N928

CPC分类号： C12N9/2457 ,  C12N9/2417 ,  C12N9/2451 ,  C12Y302/01001 ,  C12Y302/01041

摘要： The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.

摘要翻译： 本发明提供了编码具有碱性α-淀粉酶活性的碱性支链淀粉酶的DNA片段，具有SEQ ID NO：3所示的氨基酸序列的碱性α-淀粉酶和编码具有氨基酸序列的淀粉酶，碱基支链淀粉酶的DNA片段 和编码支链淀粉酶的DNA片段，含有这些DNA片段的重组DNA和含有重组DNA的转化微生物。 本发明的技术能够大量生产显示碱性α-淀粉酶活性的碱性支链淀粉酶。

公开(公告)号：US5635468A

公开(公告)日：1997-06-03

申请号：US362493

申请日：1995-01-11

申请人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Mikio Takaiwa ,  Takaaki Uemura ,  Shuji Kawai ,  Susumu Ito ,  Hiroshi Hagihara ,  Tohru Kobayashi ,  Atsushi Tanaka ,  Eiichi Hoshino

发明人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Mikio Takaiwa ,  Takaaki Uemura ,  Shuji Kawai ,  Susumu Ito ,  Hiroshi Hagihara ,  Tohru Kobayashi ,  Atsushi Tanaka ,  Eiichi Hoshino

IPC分类号： C11D3/386 ,  C12N9/28

CPC分类号： C12N9/2417 ,  C11D3/386

摘要： The present invention relates to a liquefying alkaline .alpha.-amylase having the enzymatic properties described below, a production process thereof and a detergent composition containing the same.1) Action:It hydrolyzes .alpha.-1,4-glucosidic linkages in starches, amylose, amylopectin and partial degradation products thereof and from amylose, forms glucose (G1), maltose (G2), maltotriose (G3), maltotetraose (G4), maltopentaose (G5) and maltohexaose (G6). It however does not act on pullulan.2) Isoelectric point:It has an isoelectric point higher than 8.5 when measured by an isoelectric focusing electrophoresis.The amylase according to the present invention has a liquefying activity capable of permitting degrading starches and starchy polysaccharides at high random, and has an optimum pH on the alkaline side. Owing to the high isoelectric point, it can be purified readily. Detergents with the amylase incorporated therein have excellent detergency especially against the soil of smeared food.

摘要翻译： PCT No.PCT / JP94 / 00805 Sec。 371 1995年1月11日第 102（e）日期1995年1月11日PCT 1994年5月19日PCT PCT。 第WO94 / 26881号公报 日期1994年11月24日本发明涉及具有下述酶特性的液化碱性α-淀粉酶，其制备方法和含有该酶的性质的洗涤剂组合物。 1）作用：在淀粉，直链淀粉，支链淀粉及其部分降解产物和直链淀粉中水解α-1,4-糖苷键，形成葡萄糖（G1），麦芽糖（G2），麦芽三糖（G3），麦芽四糖（G4）， 麦芽五糖（G5）和麦芽六糖（G6）。 然而，它不对支链淀粉采取行动。 2）等电点：通过等电聚焦电泳测量时，其等电点高于8.5。 根据本发明的淀粉酶具有能够允许高随机降解淀粉和淀粉多糖的液化活性，并且在碱性侧具有最佳pH。 由于等电点高，可以很容易地进行纯化。 其中掺入淀粉酶的洗涤剂具有优异的去污力，特别是对于涂抹食物的土壤。

公开(公告)号：US5147796A

公开(公告)日：1992-09-15

申请号：US825314

申请日：1992-01-27

申请人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

发明人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

IPC分类号： C12N9/44

CPC分类号： C12N9/2417 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

摘要： An isolated alkaline pullulanase Y having .alpha.-amylase activity; a microorganism producing the alkaline pullulanase Y; and a process for producing the alkaline pullulanase Y are disclosed. The alkaline pullulanase Y having .alpha.-amylase activity has its optimum pH at higher alkaline range than conventional alkaline pullulanases and exhibits excellent stability in a wide pH range. Further, the alkaline pullulanase Y has strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. Thus the alkaline pullulanase Y can advantageously be used as a detergent component.

摘要翻译： 具有α-淀粉酶活性的分离的碱性支链淀粉酶Y; 产生碱性支链淀粉酶Y的微生物; 并公开了制备碱性支链淀粉酶Y的方法。 具有α-淀粉酶活性的碱性支链淀粉酶Y具有比常规碱性支链淀粉酶更高的碱性范围的最佳pH，并且在宽pH范围内表现出优异的稳定性。 此外，碱性支链淀粉酶Y对几乎所有的洗涤剂组分如表面活性剂，螯合剂，用于洗涤剂的蛋白酶等都具有很强的抗性。 因此，碱性支链淀粉酶Y可以有利地用作洗涤剂组分。

公开(公告)号：US5147795A

公开(公告)日：1992-09-15

申请号：US575434

申请日：1990-08-30

申请人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

发明人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

IPC分类号： C12N9/44

CPC分类号： C12Y302/01041 ,  C12N9/2457 ,  Y10S435/832

摘要： A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50.degree. C. and is thermally stable up to 40.degree. C. The alkaline pullulanase has also strong reistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with .alpha.-amylase for producing various monosaccharides.

公开(公告)号：US4943532A

公开(公告)日：1990-07-24

申请号：US126739

申请日：1987-11-30

申请人： Shuji Kawai ,  Kazushi Oshino ,  Hiromi Okoshi ,  Hajime Mori ,  Katsutoshi Ara ,  Susumu Ito ,  Kikuhiko Okamoto

发明人： Shuji Kawai ,  Kazushi Oshino ,  Hiromi Okoshi ,  Hajime Mori ,  Katsutoshi Ara ,  Susumu Ito ,  Kikuhiko Okamoto

IPC分类号： C12N9/42

CPC分类号： C12R1/07 ,  C12N9/2437 ,  C12Y302/01004 ,  Y10S435/832

摘要： Novel alkali-resistant cellulases which have an optimum pH at a neutral region and have a high relative activity in an alkaline region are produced by microorganisms belonging to the genus Bacillus and growing in neutral medium.The cellulases suffer little inhibition by means of detergent ingredients such as surface active agents, proteinases and chelating agents. Therefore, they can be conveniently used as one of ingredients for detergent compositions.

摘要翻译： 在中性区域具有最佳pH并且在碱性区域中具有高相对活性的新型耐碱性纤维素酶由属于芽孢杆菌属的微生物产生并在中性培养基中生长。 纤维素酶几乎不受洗涤剂成分如表面活性剂，蛋白酶和螯合剂的抑制。 因此，它们可以方便地用作洗涤剂组合物的成分之一。

公开(公告)号：US20120183998A1

公开(公告)日：2012-07-19

申请号：US13499480

申请日：2010-10-18

申请人： Akihito Kawahara ,  Hiroshi Kodama ,  Katsutoshi Ara

发明人： Akihito Kawahara ,  Hiroshi Kodama ,  Katsutoshi Ara

IPC分类号： C12N15/113 ,  C12P19/34 ,  C12N1/21 ,  C12N15/63 ,  C12N9/38

CPC分类号： C12N15/75 ,  C12N1/20 ,  C12N15/11 ,  C12N15/66 ,  C12P21/02

摘要： The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.

摘要翻译： 本发明提供一种修饰的启动子，表达载体和各自含有启动子的转化体，以及使用该转化体产生感兴趣的基因产物的方法。 本发明提供一种修饰的启动子，其包含来源于属于芽孢杆菌属的细菌的启动子的核苷酸序列，其中至少一个选自以下的核苷酸序列已被修饰：由SEQ ID NO：1表示的核苷酸序列; 与SEQ ID NO：1所示的核苷酸序列相当的核苷酸序列，除了其中一个或多个碱基被取代，缺失，添加或插入; 和相对于由SEQ ID NO：1表示的核苷酸序列具有70％以上的序列同一性的核苷酸序列。

公开(公告)号：US20100093032A1

公开(公告)日：2010-04-15

申请号：US12530710

申请日：2008-04-01

申请人： Katsutoshi Ara ,  Hiroshi Kakeshita ,  Kouji Nakamura

发明人： Katsutoshi Ara ,  Hiroshi Kakeshita ,  Kouji Nakamura

IPC分类号： C12P21/06 ,  C12N1/00

CPC分类号： C07K14/32 ,  C12P21/02

摘要： To provide a microorganism with enhanced secretory production of a protein or polypeptide and a method of producing the protein or polypeptide using the microorganism. A modified microorganism that has been genetically modified to delete 60 to 80 carboxyl-terminal amino acids of SecA.

摘要翻译： 提供具有增强的蛋白质或多肽的分泌生产的微生物和使用该微生物产生蛋白质或多肽的方法。 已被遗传修饰以修复SecA的60至80个羧基末端氨基酸的经修饰的微生物。

公开(公告)号：US06509183B1

公开(公告)日：2003-01-21

申请号：US08307589

申请日：1994-09-28

申请人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Shuji Kawai ,  Susumu Ito

发明人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N944

CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12Y302/01041

摘要： A DNA fragment coding for alkaline pullulanase, which contains about 6.3 Kb base pairs and has a restriction map shown in FIG. 2, a recombinant vector containing the DNA fragment, and a microorganism carrying the vector. Also disclosed is a method of preparing alkaline pullulanase which comprises culturing a transformant microorganism which has been transformed with a recombinant vector containing a DNA fragment coding for alkaline pullulanase, having a restriction enzyme map shown in FIG. 2 and having about 6.3 Kb base pairs. According to the method of the invention, alkaline pullulanase which is useful as a component of detergents can be mass-produced in a low cost.

摘要翻译： 编码碱性支链淀粉酶的DNA片段，其含有约6.3Kb碱基对并且具有图1所示的限制性图谱。 2，含有DNA片段的重组载体和携带载体的微生物。还公开了制备碱性支链淀粉酶的方法，其包括培养已经用含有编码碱性支链淀粉酶的DNA片段的重组载体转化的转化体微生物，其具有 图1所示的限制酶图谱。 并且具有约6.3Kb碱基对。根据本发明的方法，可以以低成本批量生产用作洗涤剂组分的碱性支链淀粉酶。