公开(公告)号：US5635468A

公开(公告)日：1997-06-03

申请号：US362493

申请日：1995-01-11

申请人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Mikio Takaiwa ,  Takaaki Uemura ,  Shuji Kawai ,  Susumu Ito ,  Hiroshi Hagihara ,  Tohru Kobayashi ,  Atsushi Tanaka ,  Eiichi Hoshino

发明人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Mikio Takaiwa ,  Takaaki Uemura ,  Shuji Kawai ,  Susumu Ito ,  Hiroshi Hagihara ,  Tohru Kobayashi ,  Atsushi Tanaka ,  Eiichi Hoshino

IPC分类号： C11D3/386 ,  C12N9/28

CPC分类号： C12N9/2417 ,  C11D3/386

摘要： The present invention relates to a liquefying alkaline .alpha.-amylase having the enzymatic properties described below, a production process thereof and a detergent composition containing the same.1) Action:It hydrolyzes .alpha.-1,4-glucosidic linkages in starches, amylose, amylopectin and partial degradation products thereof and from amylose, forms glucose (G1), maltose (G2), maltotriose (G3), maltotetraose (G4), maltopentaose (G5) and maltohexaose (G6). It however does not act on pullulan.2) Isoelectric point:It has an isoelectric point higher than 8.5 when measured by an isoelectric focusing electrophoresis.The amylase according to the present invention has a liquefying activity capable of permitting degrading starches and starchy polysaccharides at high random, and has an optimum pH on the alkaline side. Owing to the high isoelectric point, it can be purified readily. Detergents with the amylase incorporated therein have excellent detergency especially against the soil of smeared food.

摘要翻译： PCT No.PCT / JP94 / 00805 Sec。 371 1995年1月11日第 102（e）日期1995年1月11日PCT 1994年5月19日PCT PCT。 第WO94 / 26881号公报 日期1994年11月24日本发明涉及具有下述酶特性的液化碱性α-淀粉酶，其制备方法和含有该酶的性质的洗涤剂组合物。 1）作用：在淀粉，直链淀粉，支链淀粉及其部分降解产物和直链淀粉中水解α-1,4-糖苷键，形成葡萄糖（G1），麦芽糖（G2），麦芽三糖（G3），麦芽四糖（G4）， 麦芽五糖（G5）和麦芽六糖（G6）。 然而，它不对支链淀粉采取行动。 2）等电点：通过等电聚焦电泳测量时，其等电点高于8.5。 根据本发明的淀粉酶具有能够允许高随机降解淀粉和淀粉多糖的液化活性，并且在碱性侧具有最佳pH。 由于等电点高，可以很容易地进行纯化。 其中掺入淀粉酶的洗涤剂具有优异的去污力，特别是对于涂抹食物的土壤。

公开(公告)号：US5147796A

公开(公告)日：1992-09-15

申请号：US825314

申请日：1992-01-27

申请人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

发明人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

IPC分类号： C12N9/44

CPC分类号： C12N9/2417 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

摘要： An isolated alkaline pullulanase Y having .alpha.-amylase activity; a microorganism producing the alkaline pullulanase Y; and a process for producing the alkaline pullulanase Y are disclosed. The alkaline pullulanase Y having .alpha.-amylase activity has its optimum pH at higher alkaline range than conventional alkaline pullulanases and exhibits excellent stability in a wide pH range. Further, the alkaline pullulanase Y has strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. Thus the alkaline pullulanase Y can advantageously be used as a detergent component.

摘要翻译： 具有α-淀粉酶活性的分离的碱性支链淀粉酶Y; 产生碱性支链淀粉酶Y的微生物; 并公开了制备碱性支链淀粉酶Y的方法。 具有α-淀粉酶活性的碱性支链淀粉酶Y具有比常规碱性支链淀粉酶更高的碱性范围的最佳pH，并且在宽pH范围内表现出优异的稳定性。 此外，碱性支链淀粉酶Y对几乎所有的洗涤剂组分如表面活性剂，螯合剂，用于洗涤剂的蛋白酶等都具有很强的抗性。 因此，碱性支链淀粉酶Y可以有利地用作洗涤剂组分。

公开(公告)号：US5147795A

公开(公告)日：1992-09-15

申请号：US575434

申请日：1990-08-30

申请人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

发明人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

IPC分类号： C12N9/44

CPC分类号： C12Y302/01041 ,  C12N9/2457 ,  Y10S435/832

摘要： A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50.degree. C. and is thermally stable up to 40.degree. C. The alkaline pullulanase has also strong reistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with .alpha.-amylase for producing various monosaccharides.

公开(公告)号：US06509183B1

公开(公告)日：2003-01-21

申请号：US08307589

申请日：1994-09-28

申请人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Shuji Kawai ,  Susumu Ito

发明人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N944

CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12Y302/01041

摘要： A DNA fragment coding for alkaline pullulanase, which contains about 6.3 Kb base pairs and has a restriction map shown in FIG. 2, a recombinant vector containing the DNA fragment, and a microorganism carrying the vector. Also disclosed is a method of preparing alkaline pullulanase which comprises culturing a transformant microorganism which has been transformed with a recombinant vector containing a DNA fragment coding for alkaline pullulanase, having a restriction enzyme map shown in FIG. 2 and having about 6.3 Kb base pairs. According to the method of the invention, alkaline pullulanase which is useful as a component of detergents can be mass-produced in a low cost.

摘要翻译： 编码碱性支链淀粉酶的DNA片段，其含有约6.3Kb碱基对并且具有图1所示的限制性图谱。 2，含有DNA片段的重组载体和携带载体的微生物。还公开了制备碱性支链淀粉酶的方法，其包括培养已经用含有编码碱性支链淀粉酶的DNA片段的重组载体转化的转化体微生物，其具有 图1所示的限制酶图谱。 并且具有约6.3Kb碱基对。根据本发明的方法，可以以低成本批量生产用作洗涤剂组分的碱性支链淀粉酶。

公开(公告)号：US5429766A

公开(公告)日：1995-07-04

申请号：US139148

申请日：1993-10-21

申请人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

发明人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

IPC分类号： C11D3/386 ,  C12N9/44 ,  C11D1/00

CPC分类号： C11D3/38636 ,  C12N9/2451 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

摘要： A novel detergent composition containing an alkaline pullulanase is disclosed. The alkaline pullulanase has its optimum pH in an alkaline range and not deactivated by surfactants. Further it has strong resistance to almost all detergent components such as chelating agents, protease, etc. The the detergent composition of this invention has excellent detergency especially against starch soils.

摘要翻译： 公开了一种含有碱基支链淀粉酶的新型洗涤剂组合物。 碱性支链淀粉酶在碱性范围内具有最佳pH并且不被表面活性剂失活。 此外，它对几乎所有的洗涤剂组分如螯合剂，蛋白酶等具有很强的抗性。本发明的洗涤剂组合物具有优异的去污力，特别是对淀粉土壤的去污力。

公开(公告)号：US5316691A

公开(公告)日：1994-05-31

申请号：US960262

申请日：1992-10-13

申请人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

发明人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

IPC分类号： C11D3/386 ,  C12N9/44 ,  C11D1/00 ,  C11D3/00 ,  C11D17/00

CPC分类号： C11D3/38636 ,  C12N9/2451 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

摘要： A detergent composition containing an alkaline pullulanase, a surfactant, alkaline agents and/or inorganic electrolytes, divalent metal ion scavengers and bleaching agents is disclosed. The alkaline pullulanase has an optimum pH range of 8.5-10.0 on pullulan, an optimum temperature of about 50.degree. C. and is not deactivated by surfactants. Further, the pullulanase has a strong resistance to almost all detergent components such as chelating agents, proteases, etc. The pullulanase is isolated from Bacillus sp. KSM-AP 1378 deposited as FERM BP-3048. The composition specifically contains 0.1-10 wt. % alkaline pullulanase B, 0.5-60 wt. % surfactant, 0-90 wt. % alkaline agents and/or inorganic electrolytes, 0-50 wt. % divalent metal ion scavengers, and 0-85 wt. % bleaching agents.

摘要翻译： 公开了含有碱性支链淀粉酶，表面活性剂，碱剂和/或无机电解质，二价金属离子清除剂和漂白剂的洗涤剂组合物。 碱性支链淀粉酶在支链淀粉上的最适pH范围为8.5-10.0，最适温度为约50℃，不被表面活性剂失活。 此外，支链淀粉酶对几乎所有的洗涤剂组分如螯合剂，蛋白酶等都具有很强的抗性。支链淀粉酶从芽孢杆菌中分离出来。 KSM-AP 1378保存为FERM BP-3048。 该组合物具体含有0.1-10重量％ ％碱性支链淀粉酶B，0.5-60wt。 ％表面活性剂，0-90重量％ ％碱性试剂和/或无机电解质，0-50wt。 ％的二价金属离子清除剂，和0-85wt。 ％漂白剂。

公开(公告)号：US06338959B1

公开(公告)日：2002-01-15

申请号：US09514302

申请日：2000-02-28

申请人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N928

CPC分类号： C12N9/2457 ,  C12N9/2417 ,  C12N9/2451 ,  C12Y302/01001 ,  C12Y302/01041

摘要： The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.

摘要翻译： 本发明提供了编码具有碱性α-淀粉酶活性的碱性支链淀粉酶的DNA片段，具有SEQ ID NO：3所示的氨基酸序列的碱性α-淀粉酶和编码具有氨基酸序列的淀粉酶，碱基支链淀粉酶的DNA片段 和编码支链淀粉酶的DNA片段，含有这些DNA片段的重组DNA和含有重组DNA的转化微生物。 本发明的技术能够大量生产显示碱性α-淀粉酶活性的碱性支链淀粉酶。

公开(公告)号：US06689874B2

公开(公告)日：2004-02-10

申请号：US10014436

申请日：2001-12-14

申请人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C07H2104

CPC分类号： C12N9/2457 ,  C12N9/2417 ,  C12N9/2451 ,  C12Y302/01001 ,  C12Y302/01041

摘要： The present invention provides: a nucleotide sequence encoding alkaline pullulanase and exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity; a nucleotide sequence encoding alkaline &agr;-amylase possessing an amino acid sequence described in SEQ ID NO:3; a nucleotide sequence encoding alkaline pullulanase possessing an amino acid sequence described in SEQ ID NO:4; recombinant DNAs containing these nucleotide sequences, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity.

摘要翻译： 本发明提供：编码碱基支链淀粉酶并表现出碱性α-淀粉酶和碱性支链淀粉酶活性的核苷酸序列; 编码具有SEQ ID NO：3所述的氨基酸序列的碱性α-淀粉酶的核苷酸序列; 编码具有SEQ ID NO：4所述的氨基酸序列的碱基支链淀粉酶的核苷酸序列; 含有这些核苷酸序列的重组DNA和携带重组DNA的转化微生物。 本发明的技术能够大量生产表现出碱性α-淀粉酶和碱性支链淀粉酶活性的碱性支链淀粉酶。

公开(公告)号：US20080050807A1

公开(公告)日：2008-02-28

申请号：US10829331

申请日：2004-04-22

申请人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

发明人： Yuji Hatada ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

IPC分类号： C12N15/00 ,  C07H21/04

CPC分类号： C12N9/2417

摘要： The present invention provides a DNA fragment encoding alkaline liquefying α-amylase, recombinant DNA containing the DNA fragment, a transformed microorganism harboring the recombinant DNA, as well as a method for producing alkaline liquefying α-amylase using the transformant. The method of the present invention enables mass production of alkaline liquefying α-amylase useful as a detergent component.The present invention is directed to a liquefying alkaline alphα-amylase, and a DNA encoding for the same and functional fragments thereof.

摘要翻译： 本发明提供了编码碱液化α-淀粉酶的DNA片段，含有DNA片段的重组DNA，携带重组DNA的转化微生物以及使用该转化体产生碱液化α-淀粉酶的方法。 本发明的方法能够大量生产用作洗涤剂组分的碱性液化α-淀粉酶。 本发明涉及液化碱性α-淀粉酶和编码其相同功能片段的DNA。

公开(公告)号：US4943532A

公开(公告)日：1990-07-24

申请号：US126739

申请日：1987-11-30

申请人： Shuji Kawai ,  Kazushi Oshino ,  Hiromi Okoshi ,  Hajime Mori ,  Katsutoshi Ara ,  Susumu Ito ,  Kikuhiko Okamoto

发明人： Shuji Kawai ,  Kazushi Oshino ,  Hiromi Okoshi ,  Hajime Mori ,  Katsutoshi Ara ,  Susumu Ito ,  Kikuhiko Okamoto

IPC分类号： C12N9/42

CPC分类号： C12R1/07 ,  C12N9/2437 ,  C12Y302/01004 ,  Y10S435/832

摘要： Novel alkali-resistant cellulases which have an optimum pH at a neutral region and have a high relative activity in an alkaline region are produced by microorganisms belonging to the genus Bacillus and growing in neutral medium.The cellulases suffer little inhibition by means of detergent ingredients such as surface active agents, proteinases and chelating agents. Therefore, they can be conveniently used as one of ingredients for detergent compositions.

摘要翻译： 在中性区域具有最佳pH并且在碱性区域中具有高相对活性的新型耐碱性纤维素酶由属于芽孢杆菌属的微生物产生并在中性培养基中生长。 纤维素酶几乎不受洗涤剂成分如表面活性剂，蛋白酶和螯合剂的抑制。 因此，它们可以方便地用作洗涤剂组合物的成分之一。