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公开(公告)号：US20120183998A1
公开(公告)日：2012-07-19
申请号：US13499480
申请日：2010-10-18
申请人： Akihito Kawahara , Hiroshi Kodama , Katsutoshi Ara
发明人： Akihito Kawahara , Hiroshi Kodama , Katsutoshi Ara
IPC分类号： C12N15/113 , C12P19/34 , C12N1/21 , C12N15/63 , C12N9/38
摘要： The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.
摘要翻译： 本发明提供一种修饰的启动子，表达载体和各自含有启动子的转化体，以及使用该转化体产生感兴趣的基因产物的方法。 本发明提供一种修饰的启动子，其包含来源于属于芽孢杆菌属的细菌的启动子的核苷酸序列，其中至少一个选自以下的核苷酸序列已被修饰：由SEQ ID NO：1表示的核苷酸序列; 与SEQ ID NO：1所示的核苷酸序列相当的核苷酸序列，除了其中一个或多个碱基被取代，缺失，添加或插入; 和相对于由SEQ ID NO：1表示的核苷酸序列具有70％以上的序列同一性的核苷酸序列。
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公开(公告)号：US09029519B2
公开(公告)日：2015-05-12
申请号：US13499480
申请日：2010-10-18
申请人： Akihito Kawahara , Hiroshi Kodama , Katsutoshi Ara
发明人： Akihito Kawahara , Hiroshi Kodama , Katsutoshi Ara
摘要： The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.
摘要翻译： 本发明提供一种修饰的启动子，表达载体和各自含有启动子的转化体，以及使用该转化体产生感兴趣的基因产物的方法。 本发明提供一种修饰的启动子，其包含来源于属于芽孢杆菌属的细菌的启动子的核苷酸序列，其中至少一个选自以下的核苷酸序列已被修饰：由SEQ ID NO：1表示的核苷酸序列; 与SEQ ID NO：1所示的核苷酸序列相当的核苷酸序列，除了其中一个或多个碱基被取代，缺失，添加或插入; 和相对于由SEQ ID NO：1表示的核苷酸序列具有70％以上的序列同一性的核苷酸序列。
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公开(公告)号：US20110151567A1
公开(公告)日：2011-06-23
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
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公开(公告)号：US20100255534A1
公开(公告)日：2010-10-07
申请号：US12528039
申请日：2007-12-27
申请人： Keiji Endo , Katsutoshi Ara
发明人： Keiji Endo , Katsutoshi Ara
摘要： Provision of a recombinant microorganism which has increased productivity of a protein or polypeptide of interest and a method for producing a protein or polypeptide of interest using the recombinant microorganism. The recombinant microorganism is produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the microorganism strain is prepared by: introducing a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism into the upstream of a Bacillus subtilis prsA gene or a gene corresponding thereto in the genome of a parental microorganism, or introducing a gene fragment prepared by ligating a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism to the upstream of the Bacillus subtilis prsA gene or a gene corresponding thereto into the genome of a parental microorganism; and deleting or inactivating one or more genes selected from an abrB gene, a dltA gene, a dltB gene, a dltC gene, a dltD gene, a dltE gene, and a gene (genes) corresponding thereto.
摘要翻译： 提供具有增加的目的蛋白质或多肽的生产力的重组微生物以及使用重组微生物产生感兴趣的蛋白质或多肽的方法。 通过将编码目的蛋白质或多肽的基因转移到微生物菌株来产生重组微生物，其中微生物菌株通过以下方式制备：引入在微生物中起作用的转录起始调节区或转录起始调节区和核糖体 在微生物中起作用的枯草芽孢杆菌prsA基因或与亲本微生物基因组相对应的基因的上游的引入位点，或引入通过连接在微生物中起作用的转录起始调节区或两者兼有的基因片段 转录起始调节区和在微生物中起作用的枯草芽孢杆菌prsA基因上游的核糖体结合位点或与之相对应的基因转化为亲本微生物的基因组; 以及删除或失活一个或多个选自abrB基因，dltA基因，dltB基因，dltC基因，dltD基因，dltE基因和与其相对应的基因（基因）的基因。
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公开(公告)号：US20090317346A1
公开(公告)日：2009-12-24
申请号：US12548998
申请日：2009-08-27
申请人： Syunichi Akiba , Katsutoshi Ara , Hiroshi Kusuoku , Toyoki Hagura
发明人： Syunichi Akiba , Katsutoshi Ara , Hiroshi Kusuoku , Toyoki Hagura
CPC分类号： A61K36/756 , A61K8/97 , A61K36/16 , A61Q15/00 , A61K2300/00
摘要： This invention relates to deodorant agents, which contain ginkgo or Phellodendron Bark or its extract as an active ingredient.According to the present invention, deodorant agents which are high in safety and can radically inhibit the emission of human body malodors typified by sweat odor, especially, axillary odor.
摘要翻译： 本发明涉及含有银杏或黄柏树皮或其提取物作为活性成分的除臭剂。 根据本发明，安全性高，可以根本上抑制由汗水气味，特别是腋臭引起的人体恶臭的排放的除臭剂。
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公开(公告)号：US20080050807A1
公开(公告)日：2008-02-28
申请号：US10829331
申请日：2004-04-22
申请人： Yuji Hatada , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
发明人： Yuji Hatada , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
CPC分类号： C12N9/2417
摘要： The present invention provides a DNA fragment encoding alkaline liquefying α-amylase, recombinant DNA containing the DNA fragment, a transformed microorganism harboring the recombinant DNA, as well as a method for producing alkaline liquefying α-amylase using the transformant. The method of the present invention enables mass production of alkaline liquefying α-amylase useful as a detergent component.The present invention is directed to a liquefying alkaline alphα-amylase, and a DNA encoding for the same and functional fragments thereof.
摘要翻译： 本发明提供了编码碱液化α-淀粉酶的DNA片段，含有DNA片段的重组DNA，携带重组DNA的转化微生物以及使用该转化体产生碱液化α-淀粉酶的方法。 本发明的方法能够大量生产用作洗涤剂组分的碱性液化α-淀粉酶。 本发明涉及液化碱性α-淀粉酶和编码其相同功能片段的DNA。
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公开(公告)号：US20050049308A1
公开(公告)日：2005-03-03
申请号：US10899155
申请日：2004-07-27
CPC分类号： A61K31/202
摘要： The invention contemplates drugs useful for maintenance or promotion at sites of regenerating nerves and for prevention or treatment of pathological conditions caused by denaturation or cell damage in the nervous system. Thus, the invention provides an apolipoprotein D degradation inhibitor containing lauric acid or oleic acid as an active ingredient.
摘要翻译： 本发明考虑了用于在再生神经的部位进行维持或促进的药物和用于预防或治疗由神经系统变性或细胞损伤引起的病理状况的药物。 因此，本发明提供含有月桂酸或油酸作为活性成分的载脂蛋白D降解抑制剂。
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8.发明申请Method of Modifying Target Region in Host DNA and Selectable Marker Cassette 有权修改宿主DNA和可选择标记盒中目标区域的方法公开(公告)号：US20110014709A1
公开(公告)日：2011-01-20
申请号：US12933766
申请日：2009-03-23
CPC分类号： C12N15/1082 , C12N15/102
摘要： A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5′-side region outside of the target region in the host DNA, a 3′-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3′-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5′-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3′-side region derived from the host DNA and the 3′-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter: and a selectable marker cassette for use in the method.
摘要翻译： 使用供体DNA修饰宿主DNA中的靶区域的方法：其中具有与宿主DNA中的靶区域外侧的5'-侧区域同源的区域的供体DNA，靶标外的3'侧区域 区域，宿主DNA中的靶区域内的第一同源重组区域，并且还具有第一选择标记基因，表达诱导启动子和第二选择标记基因 控制与3'侧区域同源的区域与与第一同源重组区域同源的区域之间的表达诱导启动子; 该方法具有以下步骤：在5'-侧区域和第一同源重组区域的区域上进行供体DNA和宿主DNA之间的同源重组的第一步骤，以进行与供体DNA整合的宿主的选择 基于第一选择标记基因的表达; 以及在源自宿主DNA的3'-侧区域的两个区域和源自供体DNA的3'侧区域之间，通过第1步在与供体DNA整合的宿主DNA内进行同源重组的第2步骤 ，用于在表达诱导启动子的表达诱导条件下，基于第二选择标记基因的表达来选择其目标区域被修饰的宿主;以及用于该方法的可选择标记盒。
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9.发明授权Gene for enzyme having both alkaline pullulanase and alkaline &agr;-amylase activities 失效具有碱性支链淀粉酶和碱性α-淀粉酶活性的酶的基因公开(公告)号：US06338959B1
公开(公告)日：2002-01-15
申请号：US09514302
申请日：2000-02-28
申请人： Yuji Hatada , Kazuaki Igarashi , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
发明人： Yuji Hatada , Kazuaki Igarashi , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
IPC分类号： C12N928
CPC分类号： C12N9/2457 , C12N9/2417 , C12N9/2451 , C12Y302/01001 , C12Y302/01041
摘要： The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.
摘要翻译： 本发明提供了编码具有碱性α-淀粉酶活性的碱性支链淀粉酶的DNA片段，具有SEQ ID NO：3所示的氨基酸序列的碱性α-淀粉酶和编码具有氨基酸序列的淀粉酶，碱基支链淀粉酶的DNA片段 和编码支链淀粉酶的DNA片段，含有这些DNA片段的重组DNA和含有重组DNA的转化微生物。 本发明的技术能够大量生产显示碱性α-淀粉酶活性的碱性支链淀粉酶。
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10.发明授权Liquefying alkaline .alpha.-amylase, process for producing the same, and detergent composition containing the same 失效液化碱性α-淀粉酶，其制备方法和含有它的洗涤剂组合物
公开(公告)号：US5635468A
公开(公告)日：1997-06-03
申请号：US362493
申请日：1995-01-11
申请人： Katsutoshi Ara , Katsuhisa Saeki , Kazuaki Igarashi , Mikio Takaiwa , Takaaki Uemura , Shuji Kawai , Susumu Ito , Hiroshi Hagihara , Tohru Kobayashi , Atsushi Tanaka , Eiichi Hoshino
发明人： Katsutoshi Ara , Katsuhisa Saeki , Kazuaki Igarashi , Mikio Takaiwa , Takaaki Uemura , Shuji Kawai , Susumu Ito , Hiroshi Hagihara , Tohru Kobayashi , Atsushi Tanaka , Eiichi Hoshino
CPC分类号： C12N9/2417 , C11D3/386
摘要： The present invention relates to a liquefying alkaline .alpha.-amylase having the enzymatic properties described below, a production process thereof and a detergent composition containing the same.1) Action:It hydrolyzes .alpha.-1,4-glucosidic linkages in starches, amylose, amylopectin and partial degradation products thereof and from amylose, forms glucose (G1), maltose (G2), maltotriose (G3), maltotetraose (G4), maltopentaose (G5) and maltohexaose (G6). It however does not act on pullulan.2) Isoelectric point:It has an isoelectric point higher than 8.5 when measured by an isoelectric focusing electrophoresis.The amylase according to the present invention has a liquefying activity capable of permitting degrading starches and starchy polysaccharides at high random, and has an optimum pH on the alkaline side. Owing to the high isoelectric point, it can be purified readily. Detergents with the amylase incorporated therein have excellent detergency especially against the soil of smeared food.
摘要翻译： PCT No.PCT / JP94 / 00805 Sec。 371 1995年1月11日第 102（e）日期1995年1月11日PCT 1994年5月19日PCT PCT。 第WO94 / 26881号公报 日期1994年11月24日本发明涉及具有下述酶特性的液化碱性α-淀粉酶，其制备方法和含有该酶的性质的洗涤剂组合物。 1）作用：在淀粉，直链淀粉，支链淀粉及其部分降解产物和直链淀粉中水解α-1,4-糖苷键，形成葡萄糖（G1），麦芽糖（G2），麦芽三糖（G3），麦芽四糖（G4）， 麦芽五糖（G5）和麦芽六糖（G6）。 然而，它不对支链淀粉采取行动。 2）等电点：通过等电聚焦电泳测量时，其等电点高于8.5。 根据本发明的淀粉酶具有能够允许高随机降解淀粉和淀粉多糖的液化活性，并且在碱性侧具有最佳pH。 由于等电点高，可以很容易地进行纯化。 其中掺入淀粉酶的洗涤剂具有优异的去污力，特别是对于涂抹食物的土壤。
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