Plant transformation and selection
    13.
    发明授权
    Plant transformation and selection 有权
    植物转化和选择

    公开(公告)号:US07960614B2

    公开(公告)日:2011-06-14

    申请号:US10861909

    申请日:2004-06-07

    IPC分类号: C12N15/29 C12N15/52 C12N15/82

    CPC分类号: C12N15/8205

    摘要: The present invention relates to a genotype-independent method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of an Agrobacterium inducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenic Eucalyptus and pine cells and regenerating stably transformed Eucalyptus and pine trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants, particularly forest trees.

    摘要翻译: 本发明涉及用于转化和选择植物外植体的基因型非依赖性方法。 转化方法包括在农杆菌诱导物存在下预培养外植体,并将转化的外植体暴露于加速芽发育的芽再生培养基中。 提供了从该转化方法产生的植物。 特别地,提供了获得转基因桉树和松树细胞并再生稳定转化的桉树和松树的方法。 本发明还提供用于选择和再生植物,特别是林木的培养基,方法和质粒。