公开(公告)号：US20230310708A1

公开(公告)日：2023-10-05

申请号：US18170669

申请日：2023-02-17

Applicant: Wake Forest University Health Sciences

Inventor： Giuseppe Orlando ,  Shay Soker

IPC: A61L27/36 ,  A61L27/38 ,  A61L27/54

CPC classification number: A61L27/3633 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/3808 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/256 ,  A61L2300/42 ,  A61L2430/26 ,  A61L2430/28 ,  A61L2430/40

Abstract: Methods for decellularizing organs and tissues in vitro and in vivo are provided, as are methods of maintaining organ and tissue frameworks and methods of recellularizing organs and tissues, thereby providing an approach to needed organs or tissues.

公开(公告)号：US20200377863A1

公开(公告)日：2020-12-03

申请号：US16076136

申请日：2017-02-09

Applicant: Wake Forest University Health Sciences

Inventor： Frank C. Marini ,  Shay Soker

IPC: C12N5/071 ,  C12M3/00 ,  C12N5/0786 ,  G01N33/50 ,  C12M3/06

Abstract: Provided herein is a model system for liver fibrosis, including a liver extracellular matrix, and a combination of mammalian liver cells (e.g., primary liver cells) on the matrix. In some embodiments, the combination of liver cells includes: (a) liver progenitor cells, (b) Kupffer cells, and (c) hepatic stellate cells. Methods of making the model system and methods of use of the model system for screening active agents are also provided.

公开(公告)号：US20170307598A1

公开(公告)日：2017-10-26

申请号：US15520766

申请日：2015-10-15

Applicant: Wake Forest University Health Sciences

Inventor： Aleksander Skardal ,  Shay Soker

IPC: G01N33/50 ,  B29C64/106 ,  C12N5/071 ,  B33Y70/00 ,  B01L3/00 ,  B33Y10/00 ,  B33Y80/00 ,  B29L31/40

CPC classification number: G01N33/5082 ,  B01L3/5027 ,  B01L2200/12 ,  B01L2300/1894 ,  B29C64/106 ,  B29K2005/00 ,  B29K2089/00 ,  B29K2995/0082 ,  B29L2031/40 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00 ,  C08B37/0057 ,  C08H6/00 ,  C08H8/00 ,  C12N5/0671 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2537/10 ,  G01N33/5014

Abstract: An extrudable hydrogel composition useful for making a three-dimensional organ construct includes a cross-linkable prepolymer, a post-deposition crosslinking group, optionally, an initiator that catalyzes the reaction between the prepolymer and said the crosslinking group; live cells (e.g., plant, animal, or microbial cells), optionally at least one one growth factor, and optionally water to balance. Methods of using the same and products so made are also described.