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公开(公告)号：US07063955B2
公开(公告)日：2006-06-20
申请号：US10292577
申请日：2002-11-12
申请人： Qiong Cheng , Luan Tao
发明人： Qiong Cheng , Luan Tao
CPC分类号： C12N9/0004 , C12P23/00
摘要： Genes have been isolated from Rhodococcus and Deinococcus which encode a specific lycopene β-cyclase capable of converting acyclic carotenoids with at least one ψ-end group to the corresponding asymmetric carotenoid containing a single β-ionone ring end group. The genes are new. Transformed host cells expressing the present genes and methods for the bio-conversion of acylic carotenoid substrates to corresponding asymmetric carotenoid are also provided.
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公开(公告)号：US20060035312A1
公开(公告)日：2006-02-16
申请号：US11200394
申请日：2005-08-09
申请人： Qiong Cheng , Luan Tao , Natalia Sedkova
发明人： Qiong Cheng , Luan Tao , Natalia Sedkova
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/52 , C12P23/00
摘要： A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.
摘要翻译： 提供了从Brevundimonas vesicularis DC263中分离的新型CrtZ类胡萝卜素羟化酶，其可用于生产羟基化类胡萝卜素。 另外，以前鉴定出来自新西伯替尼葡萄球菌的假想蛋白质已经发现具有类胡萝卜素羟化酶活性。 与以前报道的其他CrtZ羟化酶相比，羟化酶基因都表现出低的同源性。 异源宿主细胞中羟化酶的表达使得能够产生羟化的类胡萝卜素。
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公开(公告)号：US07252985B2
公开(公告)日：2007-08-07
申请号：US11015433
申请日：2004-12-17
申请人： Qiong Cheng , Luan Tao , Henry Yao
发明人： Qiong Cheng , Luan Tao , Henry Yao
CPC分类号： C12N9/0069 , C12P23/00
摘要： Novel CrtW carotenoid ketolase are provided that are useful for the production of ketocarotenoids. The ketolases genes of the present invention exhibit low homology in comparison to other CrtW ketolases previously reported. Expression of the carotenoid ketolases in heterologous hosts enabled production of canthaxanthin and astaxanthin. Coexpression experiments using divergent crtW genes resulted in increased production of the desired ketocarotenoids.
摘要翻译： 提供了可用于生产酮类胡萝卜素的新型CrtW类胡萝卜素酮醇酶。 与先前报道的其它CrtW酮酶相比，本发明的酮糖酶基因表现出低同源性。 异源宿主中类胡萝卜素酮酶的表达使得可以生产角黄素和虾青素。 使用发散crtW基因的共表达实验导致所需酮类胡萝卜素的产生增加。
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14.发明授权Bacterial hemoglobin genes and their use to increase carotenoid production 失效细菌性血红蛋白基因及其用于增加类胡萝卜素的生产公开(公告)号：US07163819B2
公开(公告)日：2007-01-16
申请号：US11397745
申请日：2006-04-04
申请人： Qiong Cheng , Michael P. Perry , Luan Tao
发明人： Qiong Cheng , Michael P. Perry , Luan Tao
CPC分类号： C12P23/00 , C07K14/805
摘要： Genes encoding bacterial oxygen binding proteins are provided. Recombinant expression of at least one of the present bacterial hemoglobin genes increased the growth characteristics and/or carotenoid production levels in microbial host cells grown under microaerobic conditions.
摘要翻译： 提供了编码细菌氧结合蛋白的基因。 至少一种本发明的细菌性血红蛋白基因的重组表达增加了在微需氧条件下生长的微生物宿主细胞中的生长特征和/或类胡萝卜素生产水平。
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公开(公告)号：US07132257B2
公开(公告)日：2006-11-07
申请号：US10886906
申请日：2004-07-08
申请人： Qiong Cheng , Pierre E. Rouviere , Wonchul Suh , Luan Tao
发明人： Qiong Cheng , Pierre E. Rouviere , Wonchul Suh , Luan Tao
CPC分类号： C12P23/00
摘要： A method for the in vivo bioconversion of cyclic carotenes having a β-ionone ring to the corresponding aryl carotene is provided. Gram negative host cells expressing a heterologous, codon-optimized gene encoding a carotene desaturase are grown in the presence of a suitable cyclic carotene substrate to effect the production of aromatic carotenoids.
摘要翻译： 提供了具有β-紫罗兰酮环到相应的芳基胡萝卜素的体内生物转化环状胡萝卜素的方法。 表达编码胡萝卜素去饱和酶的异源，密码子优化的基因的革兰氏阴性宿主细胞在合适的环状胡萝卜素底物的存在下生长，以产生芳香类胡萝卜素。
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公开(公告)号：US07105634B2
公开(公告)日：2006-09-12
申请号：US10358917
申请日：2003-02-05
申请人： Qiong Cheng , Kelley C. Norton , Luan Tao
发明人： Qiong Cheng , Kelley C. Norton , Luan Tao
摘要： Genes isolated from Methylomonas sp. 16a have been determined to play a role in the carotenoid biosynthetic pathway. Specifically, crtN2 gene has the ability to produce omega-aldehyde functional groups on carotenogenic substrates, while the ald gene produced omega carboxyl functional groups. These genes will be useful for production of high levels of functionalized carotenoid compounds, especially those produced in microorganisms which metabolize single carbon substrates.
摘要翻译： 从甲基幽门螺旋体分离的基因 16a被确定为在类胡萝卜素生物合成途径中发挥作用。 具体来说，crtN2基因能够在胡萝卜素生成底物上产生ω-醛官能团，而ald基因则产生ω-羧基官能团。 这些基因可用于生产高水平的官能化类胡萝卜素化合物，特别是在代谢单一碳底物的微生物中产生的那些。
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公开(公告)号：US07074588B2
公开(公告)日：2006-07-11
申请号：US11152747
申请日：2005-06-14
申请人： Qiong Cheng , Luan Tao
发明人： Qiong Cheng , Luan Tao
CPC分类号： C12N9/0069 , C12P23/00 , C12Y114/15802
摘要： A ketolase gene has been isolated from Rhodococcus erythropolis AN12 strain encoding a carotenoid modification enzyme of the carotenoid biosynthetic pathway. The gene and gene product are the first isolated from a Rhodococcus strain. Six conserved amino acid motifs have been identified as the characteristic of this type of ketolase enzymes. The gene and gene product of the present invention may be used in a variety of ways for the production of keto-carotenoid compounds in a variety of organisms.
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公开(公告)号：US07070952B2
公开(公告)日：2006-07-04
申请号：US10804677
申请日：2004-03-19
申请人： Qiong Cheng , Natalia Sedkova , Luan Tao
发明人： Qiong Cheng , Natalia Sedkova , Luan Tao
CPC分类号： C12N9/001 , C07H21/04 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： Genes have been isolated from Pectobacterium cypripedii encoding geranylgeranyl pyrophosphate (GGPP) synthase (CrtE), phytoene synthase (CrtB), phytoene desaturase (Crtl), lycopene cyclase (CrtY), β-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译： 已经从编码香叶基香叶基焦磷酸（GGPP）合成酶（CrtE），八氢番茄红素合酶（CrtB），八氢番茄红素去饱和酶（Crt1），番茄红素环化酶（CrtY），β-胡萝卜素羟化酶（CrtZ）和玉米黄质葡萄糖基转移酶（CrtX ）活动。 这些基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。 公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。
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公开(公告)号：US20050251881A1
公开(公告)日：2005-11-10
申请号：US11152747
申请日：2005-06-14
申请人： Qiong Cheng , Luan Tao
发明人： Qiong Cheng , Luan Tao
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/02 , C12N5/10 , C12N9/02 , C12N9/10 , C12P23/00 , C07H21/04 , A01H1/00 , C12N5/04 , C12N15/82
CPC分类号： C12N9/0069 , C12P23/00 , C12Y114/15802
摘要： A ketolase gene has been isolated from Rhodococcus erythropolis AN12 strain encoding a carotenoid modification enzyme of the carotenoid biosynthetic pathway. The gene and gene product are the first isolated from a Rhodococcus strain. Six conserved amino acid motifs have been identified as the characteristic of this type of ketolase enzymes. The gene and gene product of the present invention may be used in a variety of ways for the production of keto-carotenoid compounds in a variety of organisms.
摘要翻译： 已经从编码类胡萝卜素生物合成途径的类胡萝卜素修饰酶的红球菌AN12菌株中分离出一种酮糖酶基因。 该基因和基因产物是首先从红球菌菌株中分离出来的。 已经鉴定了六种保守的氨基酸基序作为这种类型的酮糖酶的特征。 本发明的基因和基因产物可以以各种方式用于在各种生物体中生产酮类胡萝卜素化合物。
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20.发明授权Genes of strain DC413 encoding enzymes involved in biosynthesis of carotenoid compounds 失效编码参与类胡萝卜素化合物生物合成的酶的DC413菌株基因公开(公告)号：US07288387B2
公开(公告)日：2007-10-30
申请号：US10810733
申请日：2004-03-26
申请人： Qiong Cheng , Luan Tao , Natalia Sedkova
发明人： Qiong Cheng , Luan Tao , Natalia Sedkova
CPC分类号： C12N9/001 , C07H21/04 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crtX-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译： 已经从Panteoa stewartii菌株DC413中分离出了一种类胡萝卜素生物合成基因簇，其中簇的遗传组织为crtE-idi-crtX-crtY-crtI-crtB-crtZ。 包含在该簇内的基因编码er牛儿基焦磷酸（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），玉米黄质葡萄糖基转移酶（CrtX），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（CrtI），八氢番茄红素合酶（CrtB） β-胡萝卜素羟化酶（CrtZ）。 基因簇，基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。 公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。
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