Human ABO Blood Group-Binding Lactobacilli
    11.
    发明申请
    Human ABO Blood Group-Binding Lactobacilli 有权
    人类ABO血型结合乳杆菌

    公开(公告)号:US20110104721A1

    公开(公告)日:2011-05-05

    申请号:US12987518

    申请日:2011-01-10

    IPC分类号: G01N33/554

    摘要: Lactobacillus screening methods were carried out using surface plasmon resonance spectrums and human intestinal mucin and blood group antigens as probes. A trial to set selection criteria in the above-mentioned methods of screening for lactobacilli was made to adapt the methods to mass screening, and it was discovered that lactobacilli compatible with ABO blood groups can be screened by setting 100 RU as a criterion for judging bacterial binding under certain conditions. Using 238 lactobacillus strains, the above-mentioned screening methods and tests to judge their compatibility for the use of yogurt production were carried out, to at long last specifically discover bacillus strains compatible with blood groups A, B, and O.

    摘要翻译: 使用表面等离子体共振光谱和人肠粘蛋白和血型抗原作为探针进行乳杆菌筛选方法。 对上述筛选乳酸杆菌的方法设定选择标准的试验进行了适应性筛选的方法,发现与ABO血型相容的乳杆菌可以通过设定100RU作为判断细菌的标准进行筛选 在某些条件下结合。 使用238种乳酸杆菌菌株,进行上述筛选方法和测试来判断其与酸奶生产的相容性,最后特别发现与血型A,B和O相容的芽孢杆菌菌株。

    Human ABO blood group-binding lactobacilli
    12.
    发明授权
    Human ABO blood group-binding lactobacilli 有权
    人ABO血型结合乳酸杆菌

    公开(公告)号:US07897374B2

    公开(公告)日:2011-03-01

    申请号:US11720462

    申请日:2005-12-01

    IPC分类号: C12N1/20

    摘要: Lactobacillus screening methods were carried out using surface plasmon resonance spectrums and human intestinal mucin and blood group antigens as probes. A trial to set selection criteria in the above-mentioned methods of screening for lactobacilli was made to adapt the methods to mass screening, and it was discovered that lactobacilli compatible with ABO blood groups can be screened by setting 100 RU as a criterion for judging bacterial binding under certain conditions. Using 238 lactobacillus strains, the above-mentioned screening methods and tests to judge their compatibility for the use of yogurt production were carried out, to at long last specifically discover bacillus strains compatible with blood groups A, B, and O.

    摘要翻译: 使用表面等离子体共振光谱和人肠粘蛋白和血型抗原作为探针进行乳杆菌筛选方法。 对上述筛选乳酸杆菌的方法设定选择标准的试验进行了适应性筛选的方法,发现与ABO血型相容的乳杆菌可以通过设定100RU作为判断细菌的标准进行筛选 在某些条件下结合。 使用238种乳酸杆菌菌株,进行上述筛选方法和测试来判断其与酸奶生产的相容性,最后特别发现与血型A,B和O相容的芽孢杆菌菌株。

    Method for Culturing Lactic Acid Bacterium and Method for Producing Fermented Milk
    14.
    发明申请
    Method for Culturing Lactic Acid Bacterium and Method for Producing Fermented Milk 有权
    培养乳酸杆菌的方法和发酵乳的制备方法

    公开(公告)号:US20120027888A1

    公开(公告)日:2012-02-02

    申请号:US13260254

    申请日:2010-03-26

    申请人: Kakuhei Isawa

    发明人: Kakuhei Isawa

    IPC分类号: A23C9/12 C12N1/20

    摘要: Protein in an aqueous whey solution is degraded by adding a protease to the aqueous whey solution. After that, a whey degradation medium is prepared by adding yeast extract or the like to the aqueous whey solution. The whey degradation medium is inoculated with bacteriocin-producing lactic acid bacterium and the lactic acid bacterium is cultured while the whey degradation medium is maintained at pH of not lower than 5 and not higher than 6. After the completion of the culture, the whey degradation medium (culture solution) is centrifuged to thereby separate a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has extremely high antibacterial activity (several tens of thousands AU) and is usable as a food preservative. Further, yogurt is produced by fermenting a yogurt mix to which 0.01% to 0.1% by weight of the concentrated cell suspension is added. With this process, it is possible to suppress an increase in the acidity of the yogurt.

    摘要翻译: 乳清水溶液中的蛋白质通过向乳清水溶液中加入蛋白酶来降解。 之后,通过向乳清水溶液中加入酵母提取物等制备乳清分解培养基。 用生产细菌的乳酸菌接种乳清分解培养基,培养乳酸菌,同时将乳清分解培养基保持在不低于5且不高于6的pH。培养完成后,乳清降解 将培养液(培养液)离心分离浓缩形式的含有乳酸菌的浓缩细胞悬浮液。 浓缩的细胞悬浮液具有极高的抗菌活性(几万AU),可用作食品防腐剂。 此外,通过发酵加入浓缩的细胞悬浮液的0.01重量%至0.1重量%的酸奶混合物来生产酸奶。 通过该方法,可以抑制酸奶的酸度的增加。

    Method for culturing lactic acid bacterium and method for producing fermented milk
    15.
    发明授权
    Method for culturing lactic acid bacterium and method for producing fermented milk 有权
    培养乳酸菌的方法及发酵乳的制造方法

    公开(公告)号:US08709516B2

    公开(公告)日:2014-04-29

    申请号:US13260254

    申请日:2010-03-26

    IPC分类号: A23C9/12

    摘要: A method for culturing lactic acid bacteria includes (a) adding a proteolytic enzyme to an aqueous whey solution containing whey and water to thereby prepare a culture solution containing whey degraded by the proteolytic enzyme; (b) inoculating the culture solution with Lactobacillus gasseri which is a bacteriocin-producing lactic acid bacteria; and (c) maintaining the culture solution inoculated with the bacteriocin-producing lactic acid bacteria at a pH of not lower than 5.2 and not higher than 5.8 to provide cultured lactic acid bacteria. After the completion of culturing, the culture solution is centrifuged to separate a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has extremely high antibacterial activity, several tens of thousands AU, and is usable as a food preservative. Yogurt is produced by fermenting a yogurt mix to which 0.01% to 0.1% by weight of the concentrated cell suspension is added.

    摘要翻译: 培养乳酸菌的方法包括(a)将蛋白水解酶加入到含有乳清和水的乳清溶液中,从而制备含有蛋白水解酶降解的乳清的培养液; (b)用作为细菌素的乳酸菌的加氏乳杆菌接种培养液; 和(c)将生殖细菌素乳酸菌接种的培养液维持在不低于5.2且不高于5.8的pH下,以提供培养的乳酸菌。 培养完成后,将培养液离心分离浓缩形式的含有乳酸菌的浓缩细胞悬浮液。 浓缩细胞悬浮液具有极高的抗菌活性,几万AU,可用作食品防腐剂。 通过发酵加入浓缩的细胞悬浮液的0.01%至0.1%的酸奶混合物来生产酸奶。