公开(公告)号：US11485799B2

公开(公告)日：2022-11-01

申请号：US17267489

申请日：2019-12-03

Applicant: ZHEJIANG UNIVERSITY

Inventor： Xingqian Ye ,  Weiwei Hu ,  Shiguo Chen ,  Huan Cheng ,  Donghong Liu

IPC: C08B37/00

Abstract: The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.

公开(公告)号：US20220056159A1

公开(公告)日：2022-02-24

申请号：US17299116

申请日：2020-06-09

Applicant: Zhejiang University

Inventor： Jianle Chen ,  Xingqian Ye ,  Huan Cheng ,  Shiguo Chen ,  Donghong Liu

IPC: C08B37/00

Abstract: Disclosed is a method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning, including the following steps: adding fresh citrus fruit peels to alkali/acid processing water from citrus fruit canning for extracting; filtering the resulting mixture, adding to the resulting filtrate 95% ethanol 1 to 3 times the volume of the filtrate, and then adjusting the pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; filtering the product after standing, washing the resulting precipitate with 50% to 70% ethanol, and drying and crushing, thereby obtaining pectin. The method makes full use of waste resources from citrus fruit canning, solves the problem of pollution by processing discharge water, and has the advantages of saving the preparation cost of pectin from citrus peels, and improving the solubility of recovered pectin.

公开(公告)号：US11110143B2

公开(公告)日：2021-09-07

申请号：US16754137

申请日：2018-09-27

Applicant: ZHEJIANG UNIVERSITY

Inventor： Shiguo Chen ,  Jiaqi Zheng ,  Xingqian Ye ,  Donghong Liu ,  Hua Zhang

IPC: A61K36/00 ,  A61K36/752 ,  A23L33/00 ,  A23L33/105 ,  A23L33/135 ,  A61K9/08 ,  A61K9/14

Abstract: The present disclosure discloses a method for preparing a citrus-derived complex prebiotic agent. The method comprises the following steps: removing external citrus peels after blanching citrus fruits with hot water, soaking citrus segments after splitting with an acid solution and an alkali solution to remove membranes of the citrus segments, and adjusting the pH of the obtained alkali soaking water with acid soaking water or acid to be weakly acidic or neutral to obtain the complex prebiotic agent, which has the function of reversing intestinal flora disorders. Compared with the traditional prebiotic product preparing method, the product prepared by the method contains prebiotic functional components with different targets such as pectin, flavonoids, oligosaccharides, and other fibers at the same time, and has the efficacies of regulating intestinal flora and reversing intestinal flora disorders caused by antibiotics. At the same time, the preparing method has the characteristics of simple operation and low cost, perfectly supplements a comprehensive utilization process of citrus fruit processing, solves the discharge problem of citrus fruit processing water, and generates very good social benefits.